Publikationsdatum:
2024-03-15
Beschreibung:
The specific transporters involved in maintenance of blood pH homeostasis in cephalopod molluscs have not been identified to date. Using in situ hybridization and immuno histochemical methods, we demonstrate that Na+/K+-ATPase (soNKA), a V-type H+-ATPase (soV-HA), and Na+/HCO3- cotransporter (soNBC) are co-localized in NKA-rich cells in the gills of Sepia officinalis. mRNA expression patterns of these transporters and selected metabolic genes were examined in response to moderately elevated seawater pCO2 (0.16 and 0.35 kPa) over a time-course of six weeks in different ontogenetic stages. The applied CO2 concentrations are relevant for ocean acidification scenarios projected for the coming decades. We determined strong expression changes in late stage embryos and hatchlings, with one to three log2-fold reductions in soNKA, soNBCe, socCAII and COX. In contrast, no hypercapnia induced changes in mRNA expression were observed in juveniles during both short- and long-term exposure. However a transiently increased demand of ion regulatory demand was evident during the initial acclimation reaction to elevated seawater pCO2. Gill Na+/K+-ATPase activity and protein concentration were increased by approximately 15% in during short (2-11 day), but not long term (42 day) exposure. Our findings support the hypothesis that the energy budget of adult cephalopods is not significantly compromised during long-term exposure to moderate environmental hypercapnia. However, the down regulation of ion-regulatory and metabolic genes in late stage embryos, taken together with a significant reduction in somatic growth, indicates that cephalopod early life stages are challenged by elevated seawater pCO2.
Schlagwort(e):
Acid-base regulation; Adenosine triphosphate synthase gene expression; Adenosine triphosphate synthase gene expression, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Carbonic anhydrase gene expression; Carbonic anhydrase gene expression, standard deviation; Coast and continental shelf; Comment; Containers and aquaria (20-1000 L or 〈 1 m**2); Cytochrome-c-oxydase gene expression; Cytochrome-c-oxydase gene expression, standard deviation; Cytochrome P-450 gene expression; Cytochrome P-450 gene expression, standard deviation; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Growth/Morphology; Laboratory experiment; Mollusca; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Octopine dehydrogenase gene expression; Octopine dehydrogenase gene expression, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Potentiometric open-cell titration; Precision scale (Sartorius TE64, Sartorius AG, Germany); Salinity; Salinity, standard deviation; see reference(s); Sepia officinalis; Sepia officinalis, length, mantle; Sepia officinalis, length, mantle, standard deviation; Sepia officinalis, mass; Sepia officinalis, mass, standard deviation; Sepia officinalis, mRNA gene expression; Sepia officinalis, mRNA gene expression, standard deviation; Sepia officinalis, mRNA gene expression change; Sepia officinalis, perivitelline fluid, pCO2; Sepia officinalis, perivitelline fluid, pH; Single species; Sodium/bicarbonate cotransporter gene expression; Sodium/bicarbonate cotransporter gene expression, standard deviation; Sodium/Potassium adenosine triphosphatase gene expression; Sodium/Potassium adenosine triphosphatase gene expression, standard deviation; Sodium/Potassium adenosine triphosphatase maximum; Sodium/Potassium adenosine triphosphatase maximum, standard deviation; Sodium/Potassium adenosine triphosphatase relative mRNA expression; Sodium/Potassium adenosine triphosphatase relative mRNA expression, standard deviation; Sodium/Potassium adenosine triphosphatase relative protein concentration; Sodium/Potassium adenosine triphosphatase relative protein concentration, standard deviation; Sodium driven chlorine/bicarbonate exchanger gene expression; Sodium driven chlorine/bicarbonate exchanger gene expression, standard deviation; Species; Stereomicroscopy (Leica); Temperate; Temperature, standard deviation; Temperature, water; WTW 340i pH-analyzer and WTW SenTix 81-electrode
Materialart:
Dataset
Format:
text/tab-separated-values, 2979 data points
Permalink
