Publication Date:
2024-02-02
Description:
The activities of proteinases, lipases/esterases and citrate synthase of Calanus finmarchicus copepodites (CV) were analysed. Analysis was performed at 30°C for copepods from seven stations (126-9, 127-17, 131-17, 133-6, 134-19, 135-16, 136-8). In addition, thermal profiles (5-50°C) of these enzymes were analysed for copepods from 3 stations (127-17, 133-6, 135-16). C. finmarchicus of station 127-19 have been acclimated on board to two different temperatures (4 and 15°C) for two weeks. Thermal profiles (5-60°C) of lipases/esterases and proteinases of adult females from each treatment were analysed. Groups of 10 individuals were used to prepare enzyme extracts for analysis. From each station/treatment, three groups were analysed, each of which was measured in triplicates.
The activity of proteinases was determined photometrically after Saborowski et al. (2004, hdl:10013/epic.20836), modified after Kreibich et al. (2008, doi:10.1007/s10152-008-0112-0). Azocasein was used as substrate. The lypolytic activity of lipases and esterases in the extract was analysed fluorometrically after Knotz et al. (2006, doi:10.1016/j.cbpa.2006.07.019) using 4-methylumbelliferyl butyrate as substrate. Citrate synthase activity was analysed photometrically after Stitt (1984) modified by Saborowski and Buchholz (2002) with oxaloacetic acid as substrate.
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Keywords:
Absorbance of control; Absorbance of sample; Basin Scale Analysis, Synthesis and Integration; Citrate synthase activity, dry mass; Citrate synthase activity per individual; Date; Date/Time of event; Depth, bottom/max; Depth, top/min; DEPTH, water; D-MOC; Double opening/closing plankton net; EURO-BASIN; Event label; Latitude of event; Life stage; Lipase activity, dry mass; Lipase activity per individual; Longitude of event; Maria S. Merian; MSM26; MSM26_126-9; MSM26_127-17; MSM26_127-19; MSM26_131-17; MSM26_133-6; MSM26_134-19; MSM26_135-16; MSM26_136-8; North Atlantic; Number of individuals; Proteinase activity per dry mass, Delta extinction at 366 nm; Proteinase activity per individual, Delta extinction at 366 nm; Sample ID; Spectrophotometer Thermo Scientific UV1; Taxon/taxa; Treatment; Treatment: temperature; Uniform resource locator/link to reference; WP2; WP-2 towed closing plankton net
Type:
Dataset
Format:
text/tab-separated-values, 3564 data points
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