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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 6 (1979), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 11 (1981), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 67 (1986), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We investigated the effect of Ca2+ on ethylene production in 2-cm long apical segments from primary roots of corn (Zea mays L., B73 × Missouri 17) seedlings. The seedlings were raised under different conditions of Ca2+ availability. Low-Ca and high-Ca seedlings were raised by soaking the grains and watering the seedlings with distilled water or 10 mM CaCl2, respectively. Segments from high-Ca roots produced more than twice as much ethylene as segments from low-Ca roots. Indoleacetic acid (IAA; 1 μM) enhanced ethylene production in segments from both low-Ca and high-Ca roots but auxin-induced promotion of ethylene production was consistently higher in segments from high-Ca roots. Addition of I-aminocyclopropane-I-carboxylic acid (ACC) to root segments from low-Ca seedlings doubled total ethylene production and the rate of production remained fairly constant during a 24 h period of monitoring. In segments from high-Ca seedlings ACC also increased total ethylene production but most of the ethylene was produced within the first 6 h. The data suggest that Ca2+ enhances the conversion of ACC to ethylene. The terminal 2 mm of the root tip were found to be especially important to ethylene biosynthesis by apical segments and, experiments using 45Ca2+ as tracer indicated that the apical 2 mm of the root is the region of strongest Ca2+ accumulation. Other cations such as Mn2+, Mg2+, and K+ could largely substitute for Ca2+. The significance of these findings is discussed with respect to recent evidence for gravity-induced Ca2+ redistribution and its relationship to the establishment of asymmetric growth during gravitropic curvature.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 51 (1981), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The ability of intact primary roots of corn (Zea mays L. Bear Hybrid WF 9 × 38) to adapt to growth-inhibitory concentrations of auxin was studied using a highly sensitive position sensor transducer to measure growth. The timing, concentration dependence and temperature dependence of adaptation were studied as well as the time course of loss of adaptation upon removal of auxin. The rate of root elongation is inhibited 80% within 40 min after application of 10−7M IAA. Within 90 min growth rate begins to recover. For concentrations of IAA equal to or greater than 10−7M, recovery of growth rate (adaptation) is incomplete. Corn roots show a similar pattern of adaptation to the synthetic auxins NAA and 2,4-D. The Q10 for adaptation is high (3.2) and comparable to that for root growth (3.3). Upon removal of exogenous IAA, loss of adaptation occurs with full sensitivity to the hormone regained within 20 min.Based on the auxin specificity and the Q10 for adaptation it is concluded that adaptation occurs neither by a change in the auxin degradation capacity of the root nor by a diffusional redistribution of applied auxin. It is suggested that adaptation involves metabolic processes, perhaps a metabolically dependent alteration of the number or affinity of auxin binding sites.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 16 (1981), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Sulcular penetration patterns of phenytoin (PHT) and albumin (BSA), in New Zealand white rabbits, were followed through the sulcular lining tissue, overlying gingiva, interposed connective tissue, proximating periosteum and blood over 180 minutes. The time course study revealed that a steady-state uptake of both molecules from the sulcus into the sulcular lining tissue was reached approximately 60 minutes after local tracer application. It was also found that PHT, after penetrating the sulcular lining tissue, was maintained at higher levels in gingiva than in other adjacent tissues while BSA levels were found highest in periosteum. Kinetic studies of these tissues demonstrated that sulcular tissue had a greater capacity for PHT than BSA and that uptake by this tissue had a capacity-limited characteristic. The other adjacent tissues and blood also showed capacity-limited characteristics probably reflecting uptake by the sulcular tissues. BSA degradation or PHT metabolism did not occur in any of these tissues or in serum.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 72 (1988), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Hasenstein, K. H. and Evans, M. L. 1988. The influence of calcium and pH on growth in primary roots of Zea mays. - Physiol. Plant. 72: 466–470.We investigated the interaction of Ca2+ and pH on root elongation in Zea mays L. cv. B73 × Missouri 17 and cv. Merit. Seedlings were raised to contain high levels of Ca2+ (HC, imbibed and raised in 10 mM CaCl2) or low levels of Ca2+ (LC, imbibed and raised in distilled water). In HC roots, lowering the pH (5 mM MES/Tris) from 6.5 to 4.5 resulted in strong, long-lasting growth promotion. Surprisingly, increasing the pH from 6.5 to 8.5 also resulted in strong growth promotion. In LC roots acidification of the medium (pH 6.5 to 4.5) resulted in transient growth stimulation followed by a gradual decline in the growth rate toward zero. Exposure of LC roots to high pH (pH shift from 6.5 to 8.5) also promoted growth. Addition of EGTA resulted in strong growth promotion in both LC and HC roots. The ability of EGTA to stimulate growth appeared not to be related to H+ release from EGTA upon Ca2+ chelation since, 1) LC roots showed a strong and prolonged response to EGTA, but only a transient response to acid pH, and 2) promotion of growth by EGTA was observed in strongly buffered solutions. We also examined the pH dependence of the release of 45Ca2+ from roots of 3-day-old seedlings grown from grains imbibed in 45Ca2+. Release of 45Ca2+ from the root into agar blocks placed on the root surface was greater the more acidic the pH of the blocks. The results indicate that Ca2+ may be necessary for the acid growth response in roots.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 19 (1990), S. 13-26 
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The determination of the amino acid composition and sequence of a peptide, using both conventional and tandem fast atom bombardment mass spectrometry, is presented. First a list of potential empirical formulae is generated using the accurate mass and isotopic peak intensity ratios from the molecular ion cluster. The formulae are mathematically analyzed to ensure that they correspond to linear peptides composed of 19 common amino acids. Then using the ions below m/z 160 Da, which are characteristic of the amino acid content of the peptide, the formulae are decomposed into possible amino acid compositions. From each composition sequences are generated and their predicted fragment ions are compared to ions present in the mass spectrum. A score based on the intensities and the occurrence of consecutive sequence fragments is calculated. Finally sequences with the ten highest scores are retained. Analyses performed on typical peptides with molecular weights below 1500 Da indicate that identification can generally be achieved using this approach.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 16 (1990), S. 87-88 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Publication Date: 2017-07-25
    Description: The expansion of coniferous trees into sagebrush ecosystems is a major driver of habitat loss and fragmentation, resulting in negative impacts to wildlife. Greater sage-grouse ( Centrocercus urophasianus ) respond directly to conifer expansion through decreased breeding activity, nesting, and overall survival; thus, small amounts of conifer expansion can have significant impacts on sage-grouse habitat and populations. To this end, conservation partners have collaborated across private and public lands to reduce the threat of conifer expansion through targeted removal of conifer trees. Here, we demonstrate the use of the Marxan framework to incorporate important ecosystem attributes in the prioritization of conifer removal within the Oregon range of sage-grouse. We prioritized conifer removal relative to three separate goals: (1) enhancement of existing sage-grouse breeding, nesting, and early brood-rearing habitats; (2) facilitation of sage-grouse movement between breeding and brood-rearing habitats; and (3) improvement of connectivity among sage-grouse priority areas for conservation (PACs). Optimization models successfully identified areas with low conifer canopy cover, high resilience and resistance to wildfire and annual grass invasion, and high bird abundance to enhance sage-grouse habitat. The inclusion of mesic resources resulted in further prioritization of areas that were closer to such resources, but also identified potential pathways that connected breeding habitats to the late brood-rearing habitats associated with mesic areas. Examining areas outside of PACs resulted in the selection of potential corridors to facilitate connectivity; although areas with low conifer cover were selected similarly to the other optimization models, areas with high cover were also chosen to be able to enhance connectivity. Areas identified by optimization models were largely consistent with and overlapped ongoing conifer removal efforts in the Warner Mountains of south-central Oregon. Land ownership of preferential areas selected by models varied with priority goals and followed general ownership patterns of the region, with public lands managed by the Bureau of Land Management and private lands being selected the most. The increased availability of landscape-level datasets and assessment tools in sagebrush ecosystems can reduce the time and cost of both planning and implementation of habitat projects involving conifer removal. Most importantly, incorporating these new datasets and tools can supplement expert-based knowledge to maximize benefits to sagebrush and sage-grouse conservation.
    Electronic ISSN: 2150-8925
    Topics: Energy, Environment Protection, Nuclear Power Engineering
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  • 10
    Publication Date: 2014-07-25
    Description: Single particle mass spectral data, collected in Paris, France during the MEGAPOLI winter campaign, have been used to predict hygroscopic growth at the single particle level. The mass fractions of black carbon, organic aerosol, ammonium, nitrate and sulphate present in each particle were estimated using a combination of single particle mass spectrometer and bulk aerosol chemical composition measurements. The Zdanovskii-Stokes-Robinson (ZSR) approach was then applied to predict hygroscopic growth factors based on these mass fraction estimates. Smaller particles with high black carbon mass fractions and low inorganic ion mass fractions exhibited the lowest predicted growth factors, while larger particles with high inorganic ion mass fractions exhibited the highest growth factors. Growth factors were calculated for subsaturated relative humidity (90%) to enable comparison with hygroscopic tandem differential mobility analyser (HTDMA) measurements. Mean predicted and measured hygroscopic growth factors for 110, 165 and 265 nm particles were found to agree within 6%. Single particle-based ZSR hygroscopicity estimates offer an advantage over bulk aerosol composition-based hygroscopicity estimates by providing additional chemical mixing state information. External mixing can be determined for particles of a given diameter through examination of the predicted hygroscopic growth factor distributions. 110 nm and 265 nm particles were found to be predominantly internally mixed using this approach, however external mixing of 165 nm particles was observed periodically when thinly coated and thickly coated BC particles were simultaneously detected. Single particle-resolved chemical information will be useful for modelling efforts aimed at constraining cloud condensation nuclei activity and hygroscopic growth.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
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