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  • 1
    Electronic Resource
    Electronic Resource
    Experimental study of drainage and granulation in response to the intra-articular injection of particles in rat knee joints (1997)
    Springer
    Journal of orthopaedic science 2 (1997), S. 24-30 
    Details
    ISSN: 1436-2023
    Keywords: synovial membrane ; lymphatic vessel ; particle ; drainage ; microsphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Reactive granulation and drainage of intraarticularly injected plastic particles in rat knee joints was examined by light and electron microscopy. The knee joints and associated iliac lymph nodes were excised at various intervals after the injection of latex beads (ϕ1 μm) or fluoresbrite particles (ϕ0.2 μm or ϕ10 μm) from 5 min until 3 months after the injection. Particles in the lymphatic or blood vessels were successfully demonstrated by an enzyme-histochemical method (5′-nucleotidase staining). Five min after the injection, most of the particles were scattered on the surface of the synovial membrane, and some particles were phagocytosed by synovial lining cells. After 5 h, neutrophils had phagocytosed particles which adhered to fibrin in the joint cavity. Twelve h after the injection, after the neutrophils had died, those same particles were phagocytosed by macrophages in the joint cavity. One day after the injection, Fluoresbrite particles (ϕ0.2 μm) phagocytosed by macrophages were found in the iliac lymph nodes, while latex particles (ϕ1 μm) were detected in the iliac lymph nodes 3 days after the injection. Some Fluoresbrite particles (ϕ10 μm) were seen in the 5′-nucleotidase-positive lymphatic vessels in the synovial membrane. Three months after the injection, many macrophages filled with particles had formed granulation tissue in the synovial membrane, and macrophages containing phagocytosed particles were also seen increasingly in the iliac lymph nodes. Our findings suggested that neutrophils and macrophages phagocytosed injected particles in the joint cavity, and that the macrophages brought the particles into the deep layer of the synovial membrane. Phagocytic macrophages also carried the particles to the iliac lymph nodes through lymphatic vessels in the synovial membrane. There were no morphological differences in the processes of granulation and drainage between the two different sized plastic particles (ϕ1 μm and ϕ0.2 μm), except for the behavior of the macrophages phagocytosing the particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01239755
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  • 2
    Electronic Resource
    Electronic Resource
    Brain oxygenation monitored by near-infared spectroscopy during recovery from hemorrhagic shock (1996)
    Springer
    Journal of anesthesia 10 (1996), S. 289-291 
    Details
    ISSN: 1438-8359
    Keywords: Near-infrared spectroscopy ; Brain oxygenation ; Hemorrhagic shock ; Monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02483397
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  • 3
    Electronic Resource
    Electronic Resource
    Anesthetic management for surgical repair of postinfarction ventricular septal defect (1996)
    Springer
    Journal of anesthesia 10 (1996), S. 296-299 
    Details
    ISSN: 1438-8359
    Keywords: Postinfarction ventricular septal defect ; Fentanyl anesthesia ; Heart failure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02483399
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  • 4
    Electronic Resource
    Electronic Resource
    Hypertonic saline for intraoperative fluid therapy in transurethral resection of the prostate (1996)
    Springer
    Journal of anesthesia 10 (1996), S. 170-175 
    Details
    ISSN: 1438-8359
    Keywords: Hypertonic saline ; Intraoperative fluid therapy ; Transurethral resection of the prostate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We tested hypertonic saline solution (HS) to determine its effectiveness in surgical procedures for prostatic hypertrophy. We randomly selected 40 patients undergoing elective transurethral resection of the prostate for either infusion of HS (3% NaCl) at 4ml·kg−1·min−1 (HS group) or lactated Ringer's solution (LR) at 8 ml·kg−1·min−1 (LR group). Anesthesiologists regulated the intraoperative infusion rate as needed to maintain blood pressure. There were no differences in systolic blood pressure, heart rate, central venous pressure, or arterial blood oxygenation between the two groups. In the HS group, plasma sodium, chloride, and osmolality, measured in the recovery room, were significantly increased; however, they returned to preanesthetic levels the day after surgery. In the LR group, in contrast, plasma sodium decreased significantly and this lower value persisted for 1 day. An osmolar gap exceeding 10mOsm·kg−1 was observed in 2 patients in the HS group, but plasma sodium remained at normal values. However, in the 1 patient in the LR group whose osmolar gap exceeded 10mOsm·kg−1, plasma sodium was 115 mEq·I−1. HS, at a low dose, is useful in the intraoperative management of transurethral resection of the prostate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02471385
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  • 5
    Electronic Resource
    Electronic Resource
    Intralobular lymphatic vessels and their relationship to blood vessels in the mouse thymus (1988)
    Springer
    Cell & tissue research 253 (1988), S. 181-187 
    Details
    ISSN: 1432-0878
    Keywords: Lymphatic vessels ; Thymus ; Perivascular spaces ; Postcapillary venules ; Mouse (BALB/c)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The spatial distribution and fine structure of the lymphatic vessels within the thymic lobules of normal and hydrocortisone-injected mice were studied by light- and electron microscopy. The lymphatic vessels of the cortex and medulla of normal thymus are irregularly shaped spaces closely associated with branches of the intralobular artery and vein. The overall distribution of these vessels in the greatly involuted thymus of hydrocortisone-treated mice is essentially the same as in the normal thymus. The wall of the lymphatic vessels consists of only a layer of endothelial cells supported by underlying reticular cells. The luminal surface of the endothelial cell is smooth, but trabecular processes are often seen. There are three morphological types of intercellular contacts between contiguous cells, namely, end-to-end, overlapping and interdigitating. The lymphatic vessel has anchoring filaments and collagen fibrils, but a basal lamina is either absent, or if present, is discontinuous. This is in contrast to the continuous basal lamina of the venule. The perivascular space surrounding the postcapillary venule opens into a terminal lymphatic vessel at the cortico-medullary junction and in the medulla. Lymphocytes are seen penetrating the lymphatic endothelium, particularly in acutely involuted thymuses. These findings suggest that the intralobular lymphatic vessels may originate from the vacuities that surround the postcapillary venules, and the lymphatic system may function as a pathway for the migration of lymphocytes into or out of the lymphatic circulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221753
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  • 6
    Electronic Resource
    Electronic Resource
    Fine structure and distribution of lymphatic vessels in the human dental pulp: a study using an enzyme-histochemical method (1997)
    Springer
    Cell & tissue research 288 (1997), S. 79-85 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Lymphatic vessels ; Blood vessels ; Dental pulp ; 5′-nucleotidase ; Alkaline phosphatase ; Enzyme histochemistry ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The existence of lymphatic vessels in the human dental pulp and their distribution were established by light and electron microscopy using an enzyme-histochemical method. The distinction between lymphatic and blood vessels was made by light microscopy on cryostat sections of undecalcified and decalcified teeth using 5′-nucleotidase(5′-Nase)-alkaline phosphatase double staining. On the tissue surface, 5′-Nase-positive lymphatic vessels were highlighted with good contrast and resolution by backscattered electron imaging using scanning electron microscopy. By transmission electron microscopy, dense granular precipitations resulting from the 5′-Nase reaction were seen on the luminal surface of the lymphatic endothelial cells as well as in the area at the basal side, but were absent in the blood vessels. These lymphatic vessels were more numerous in the central part than in the peripheral odontoblastic layer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050794
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  • 7
    Electronic Resource
    Electronic Resource
    Difficult laryngoscopy and cephalometric roentgenograms in obstructive sleep apnea syndrome patients undergoing uvulopalatopharyngoplasty (1996)
    Springer
    Journal of anesthesia 10 (1996), S. 260-263 
    Details
    ISSN: 1438-8359
    Keywords: Obstructive sleep apnea syndrome ; Cephalometric roentgenogram ; Difficult laryngoscopy ; Uvulopalatopharyngoplasty
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We retrospectively studied the incidence of difficult laryngoscopy in 53 subjects with obstructive sleep apnea syndrome (OSAS) undergoing uvulopalatopharyngoplasty (UPPP) and 72 subjects with chronic otitis media undergoing tympanoplasty (control group). The incidence of difficult laryngoscopy in the UPPP group was significantly higher than in the control group (18.9%vs 4.2%,P〈0.001). To analyze the anatomical findings of difficult laryngoscopy in UPPP patients, cephalometric roentgenograms were compared between patients in whom direct laryngoscopy was difficult (difficult patients,n=10) and patients in whom direct laryngoscopy was not difficult (nondifficult patients,n=43). Cephalometric atlanto-occipital distance (cAOD) was less than 4mm in 80% of the difficult patients, and there were significant differences between the difficult patients and the nondifficult patients (2.8±3.3 mmvs 6.7±3.0 mm, mean ±SD,P〈0.001). There were significant differences in cephalometric effective mandibular length/cephalometric posterior depth of mandible ratio (cEML/cPDM) between the difficult patients and the nondifficult patients (4.0±0.6vs 4.5 ±0.8,P〈0.01); however, the calculation of cEML/cPDM was more difficult than cAOD. We concluded that OSAS patients undergoing UPPP are at high risk for difficult laryngoscopy, and that cAOD calculated from cephalometric roentgenograms is an easy and sensitive predictive indicator for the estimation of difficult laryngoscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02483392
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  • 8
    Electronic Resource
    Electronic Resource
    Quantitative chromosome map of the polyploid Saccharum spontaneum by multicolor fluorescence in situ hybridization and imaging methods (1999)
    Springer
    Plant molecular biology 39 (1999), S. 1165-1173 
    Details
    ISSN: 1573-5028
    Keywords: condensation pattern ; image analysis ; multi-color FISH of 45S and 5S rDNAs ; polyploidy ; quantitative chromosome map ; Saccharum spontaneum L. ; wild sugarcane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic chromosomes of a wild relative of sugarcane (Saccharum spontaneum L.) anther culture-derived clone (AP 85-361, 2n=32) were identified and characterized by computer-aided imaging technology and molecular cytological methods. The presence of four satellite chromosomes and four nearly identical chromosome sets suggests that the clone is a tetrahaploid with the basic number x=8. A quantitative chromosome map, or idiogram, was developed using image analysis of the condensation pattern (CP) at the prometaphase stage of somatic chromosomes. The 45S and 5S ribosomal RNA gene (rDNA) loci were simultaneously visualized by multi-color fluorescence in situ hybridization (McFISH) and precisely localized to the regions of 3p3.1 and 6q1.3 on the idiogram. The simultaneous visualization of two sets of four ribosomal RNA genes confirms tetraploidy of this clone. This conclusion is consistent with results of molecular marker mapping. The quantitative chromosome map produced will become the foundation for genome analyses based on chromosome identity and structure. Previously impossible identification of small chromosomes and untestable hypotheses about the polyploid nature of plants can now be settled with these two approaches of quantitative karyotyping and FISH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006133804170
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