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  • SAGE Publications  (31)
  • 1
    Online Resource
    Online Resource
    The Identification of Prognostic and Metastatic Alternative Splicing in Skin Cutaneous Melanoma
    SAGE Publications ; 2022
    In:  Cancer Control Vol. 29 ( 2022-01), p. 107327482110515-
    Details
    In: Cancer Control, SAGE Publications, Vol. 29 ( 2022-01), p. 107327482110515-
    Abstract: Skin cutaneous melanoma (SKCM) is a type of highly invasive cancer originated from melanocytes. It is reported that aberrant alternative splicing (AS) plays an important role in the neoplasia and metastasis of many types of cancer. Therefore, we investigated whether ASEs of pre-RNA have such an influence on the prognosis of SKCM and the related mechanism of ASEs in SKCM. The RNA-seq data and ASEs data for SKCM patients were obtained from the TCGA and TCGASpliceSeq database. The univariate Cox regression revealed 1265 overall survival-related splicing events (OS-SEs). Screened by Lasso regression, 4 OS-SEs were identified and used to construct an effective prediction model (AUC: .904), whose risk score was proved to be an independent prognostic factor. Furthermore, Kruskal–Wallis test and Mann–Whitney–Wilcoxon test showed that an aberrant splicing type of aminoacyl tRNA synthetase complex-interacting multifunctional protein 2 (AIMP2) regulated by CDC-like kinase 1 (CLK1) was associated with the metastasis and stage of SKCM. Besides, the overlapped signal pathway for AIMP2 was galactose metabolism identified by the co-expression analysis. External database validation also confirmed that AIMP2, CLK1, and the galactose metabolism were associated with the metastasis and stage of SKCM patients. ChIP-seq and ATAC-seq methods further confirmed the transcription regulation of CLK1, AIMP2, and other key genes, whose cellular expression was detected by Single Cell Sequencing. In conclusion, we proposed that CLK1-regulated AIMP2-78704-ES might play a critical role in the tumorigenesis and metastasis of SKCM via galactose metabolism. Besides, we established an effective model with MTMR14-63114-ES, URI1-48867-ES, BATF2-16724-AP, and MED22-88025-AP to predict the metastasis and prognosis of SKCM patients.
    Type of Medium: Online Resource
    ISSN: 1073-2748 , 1073-2748
    URL: Article
    DOI: 10.1177/10732748211051554
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2004182-2
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  • 2
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    Establishment of a Zebrafish Xenograft Model for in Vivo Investigation of Nasopharyngeal Carcinoma
    SAGE Publications ; 2022
    In:  Cell Transplantation Vol. 31 ( 2022-01), p. 096368972211160-
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 31 ( 2022-01), p. 096368972211160-
    Abstract: Nasopharyngeal carcinoma (NPC) is a unique malignant tumor of the head and neck. Despite higher survival rates by the combination of radiotherapy and chemotherapy, the recurrence or metastasis of NPC still occurs at about 10%. Therefore, there is urgent demand to develop more effective in vivo models for preclinical trials to investigate the mechanisms of NPC development and progression and to explore better treatment approaches. In this study, we transplanted human NPC CNE1 cells into zebrafish embryos to establish a xenograft model of NPC, where the proliferation and invasion behaviors of NPC cells were investigated in vivo. Combining in vitro and in vivo analyses, we found that activating transcription factor 7 (ATF7) was involved in the occurrence and development of NPC regulated by peptidyl-prolyl cis- trans isomerase NIMA-interacting 1 (Pin1). The zebrafish NPC xenograft model established here thereby provides an in vivo tool for exploring the occurrence and development of NPC, which may help to identify new tumor markers and develop new therapeutic strategies for the treatment of NPC.
    Type of Medium: Online Resource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.1177/09636897221116085
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2020466-8
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  • 3
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    Transcriptome Analysis of Induced Pluripotent Stem Cell (iPSC)-derived Pancreatic β-like Cell Differentiation
    SAGE Publications ; 2017
    In:  Cell Transplantation Vol. 26, No. 8 ( 2017-08), p. 1380-1391
    Details
    In: Cell Transplantation, SAGE Publications, Vol. 26, No. 8 ( 2017-08), p. 1380-1391
    Abstract: Diabetes affects millions of people worldwide, and β-cell replacement is one of the promising new strategies for treatment. Induced pluripotent stem cells (iPSCs) can differentiate into any cell type, including pancreatic β cells, providing a potential treatment for diabetes. However, the molecular mechanisms underlying the differentiation of iPSC-derived β cells have not yet been fully elucidated. Here, we generated pancreatic β-like cells from mouse iPSCs using a 3-step protocol and performed deep RNA sequencing to get a transcriptional landscape of iPSC-derived pancreatic β-like cells during the selective differentiation period. We then focused on the differentially expressed genes (DEGs) during the time course of the differentiation period, and these genes underwent Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway analysis. In addition, gene-act networks were constructed for these DEGs, and the expression of pivotal genes detected by quantitative real-time polymerase chain reaction was well correlated with RNA sequence (RNA-seq). Overall, our study provides valuable information regarding the transcriptome changes in β cells derived from iPSCs during differentiation, elucidates the biological process and pathways underlying β-cell differentiation, and promotes the identification and functional analysis of potential genes that could be used for improving functional β-cell generation from iPSCs.
    Type of Medium: Online Resource
    ISSN: 0963-6897 , 1555-3892
    URL: Article
    DOI: 10.1177/0963689717720281
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
    detail.hit.zdb_id: 2020466-8
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  • 4
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    The Mangrove-Derived Diterpenoid Diaporthe B Inhibits the Stemness and Increases the Efficacy of Docetaxel in Prostate Cancer PC-3 Cells
    SAGE Publications ; 2021
    In:  Natural Product Communications Vol. 16, No. 12 ( 2021-12), p. 1934578X2110496-
    Details
    In: Natural Product Communications, SAGE Publications, Vol. 16, No. 12 ( 2021-12), p. 1934578X2110496-
    Abstract: The absolute configuration of diaporthe B, a pimarane diterpene isolated from the mangrove derived endophytic fungus Eutypella sp #3E, was determined by a single-crystal x-ray diffraction study. The present study aimed to investigate the effects of diaporthe B on docetaxel-resistant prostate cancer PC-3 cells. Results of our studies showed that docetaxel-resistant PC-3 cells had higher sphere-forming efficiency and an increase in adherence to collagen-coated culture plates. The protein levels of cancer stem cell (CSC)-related markers CD44, CD133, and ALDH1A1 were higher in the docetaxel-resistant PC-3 cells than in the parental cells. Treatment with diaporthe B dose-dependently inhibited the growth and induced apoptosis in the resistant cells. Moreover, diaporthe B treatment decreased the sphere-forming efficiency and the adherence to collagen-coated plates in docetaxel-resistant PC-3 cells. Diaporthe B also decreased the protein levels of CSC-related markers CD44, CD133, and ALDH1A1 in the resistant cells. In addition, a combination of diaporthe B and docetaxel had a more potent effect on growth inhibition and apoptosis in the resistant cells than either agent alone. Our studies suggest that diaporthe B inhibits the stemness of prostate cancer cells and may have therapeutic potential for enhancing the efficacy of docetaxel in docetaxel-resistant prostate cancer cells.
    Type of Medium: Online Resource
    ISSN: 1934-578X , 1555-9475
    URL: Article
    DOI: 10.1177/1934578X211049688
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2430442-6
    SSG: 15,3
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  • 5
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    Sacrificial template method for the synthesis of three-dimensional nanofibrous 58S bioglass scaffold and its in vitro bioactivity and cell responses
    SAGE Publications ; 2017
    In:  Journal of Biomaterials Applications Vol. 32, No. 2 ( 2017-08), p. 265-275
    Details
    In: Journal of Biomaterials Applications, SAGE Publications, Vol. 32, No. 2 ( 2017-08), p. 265-275
    Type of Medium: Online Resource
    ISSN: 0885-3282 , 1530-8022
    URL: Article
    DOI: 10.1177/0885328217715784
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2017
    detail.hit.zdb_id: 2072559-0
    SSG: 12
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  • 6
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    Citric acid coated ultrasmall superparamagnetic iron oxide nanoparticles conjugated with lactoferrin for targeted negative MR imaging of glioma
    SAGE Publications ; 2021
    In:  Journal of Biomaterials Applications Vol. 36, No. 1 ( 2021-07), p. 15-25
    Details
    In: Journal of Biomaterials Applications, SAGE Publications, Vol. 36, No. 1 ( 2021-07), p. 15-25
    Abstract: The proposed study was to develop the preparation of ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) modified with citric acid, with surface conjugated with lactoferrin (Lf), which used as a potential targeted contrast agent for magnetic resonance imaging (MRI) of brain glioma. USPIONs were prepared by the thermal decomposition method. The hydrophobic USPIONs were coated with citric acid by the ligand exchange method. Then, Lf was conjugated into the surface of USPIONs. The obtained Lf-USPIONs were analyzed by fourier transform infrared (FTIR) spectroscopy and polyacrylamide gel electrophoresis. The size, size distribution, shape and superparamagnetic property of Lf-USPIONs were investigated with TEM and vibrating sample magnetometer (VSM). Both FTIR and electrophoresis analysis demonstrated the successful conjugation of Lf to the surface of USPIONs. The average size of Lf-USPIONs was about 8.4 ± 0.5 nm, which was determined using the statistics of measured over 100 nanoparticles in the TEM image, with a negative charge of −7.3 ± 0.2 mV. TEM imaging revealed that Lf-USPIONs were good in dispersion and polygonal in morphology. VSM results indicated that Lf-USPIONs were superparamagnetic and the saturated magnetic intensity was about 69.8 emu/g. The Lf-USPIONs also showed good biocompatibility in hemolysis, cytotoxicity, cell migration and blood biochemistry studies. MR imaging results in vitro and in vivo indicated that Lf-USPIONs exhibited good negative contrast enhancement. Taken together, Lf-USPIONs hold great potential for brain gliomas MR imaging as a nanosized targeted contrast agent.
    Type of Medium: Online Resource
    ISSN: 0885-3282 , 1530-8022
    URL: Article
    DOI: 10.1177/0885328220975570
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2072559-0
    SSG: 12
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  • 7
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    Effects of polygonal wear of wheels on the dynamic performance of the gearbox housing of a high-speed train
    SAGE Publications ; 2018
    In:  Proceedings of the Institution of Mechanical Engineers, Part F: Journal of Rail and Rapid Transit Vol. 232, No. 6 ( 2018-07), p. 1852-1863
    Details
    In: Proceedings of the Institution of Mechanical Engineers, Part F: Journal of Rail and Rapid Transit, SAGE Publications, Vol. 232, No. 6 ( 2018-07), p. 1852-1863
    Abstract: This study investigates the effects of polygonal wear of wheels on the dynamic performance of the gearbox housing of high-speed trains. First, a dynamics model of the driving system is developed considering gear meshing and flexible deformation of the gearbox housing. Responses of the gearbox housing are calculated using the modal superposition method. Then, a multibody dynamics model of a railway motor car is established considering the driving system. Rig tests in the laboratory and field experiments on the Beijing–Shanghai high-speed rail line are, respectively, performed. Based on the simulation and experiments, the dynamic characteristics of the gearbox housing of the motor car are analysed. The results of the analysis show that polygonal wear can significantly influence the vibration of the gearbox housing, and the maximum amplitude of the acceleration of vibration is more than 200  g. In addition, resonance of the gearbox housing occurs due to the 20th-order polygonal wear. In the resonance region, the oil level sight glass of the gearbox housing vibrates severely, contributing immensely to its cracking.
    Type of Medium: Online Resource
    ISSN: 0954-4097 , 2041-3017
    URL: Article
    DOI: 10.1177/0954409717752998
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2018
    detail.hit.zdb_id: 2024901-9
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  • 8
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    A vision-based container position measuring system for ARMG
    SAGE Publications ; 2023
    In:  Measurement and Control Vol. 56, No. 3-4 ( 2023-03), p. 596-605
    Details
    In: Measurement and Control, SAGE Publications, Vol. 56, No. 3-4 ( 2023-03), p. 596-605
    Abstract: This paper analyzes the problems of existing container positioning methods and proposed a vision-based container position measuring system to provide precise parameters for container lifting operations. This system uses camera to get container information, then detects container corners by the method that combined with convolutional neural network and traditional image processing algorithm. This system is used to provide specific parameters associated with container lifting operation. In the first of detection, it uses the modified SSD (Single Shot MultiBox Detector) neural network to detect the coarse position of container corners in the image, second stage detection uses the usage of rectangle fitting to detect the precise position of corner holes in the coarse position. In the last step the offset distance and deflection angle were calculated by precise corner position. The experiment shows the detection rate of the proposed system reach 94%. The positioning errors between 14.3 and 19.6 mm for a frame rate of 10 fps are obtained.
    Type of Medium: Online Resource
    ISSN: 0020-2940
    URL: Article
    DOI: 10.1177/00202940221110932
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2712343-1
    SSG: 3,2
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  • 9
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    Spontaneous internal hemorrhage of a giant hepatic hemangioma with infection: a case report and literature review
    SAGE Publications ; 2020
    In:  Journal of International Medical Research Vol. 48, No. 12 ( 2020-12), p. 030006052097647-
    Details
    In: Journal of International Medical Research, SAGE Publications, Vol. 48, No. 12 ( 2020-12), p. 030006052097647-
    Abstract: Spontaneous internal hemorrhage from a hepatic hemangioma is rare. This case describes a 59-year-old woman who was hospitalized with recurrent cough and fever for 6 months. The doctor thought that she had pneumonia, but other infectious diseases could not be ruled out. Therefore, related tests were performed and strong antibiotics were used, but the symptom of fever was persistent and recurred. Enhanced computed tomography (CT) findings showed a right hepatic giant hemangioma with hemorrhage, while tuberculosis, liver abscess, and immune disease were excluded by the physician. Because the patient’s fever was associated with a large hepatic hemangioma, the patient was transferred to surgery. During treatment, the amount of bleeding increased, so she underwent a right hepatic hemangioma resection in the emergency department. Her postoperative fever symptoms subsequently resolved. Pathological examination confirmed hemorrhagic necrosis with infection in hepatic hemangioma. Follow-up showed that the patient was afebrile.
    Type of Medium: Online Resource
    ISSN: 0300-0605 , 1473-2300
    URL: Article
    DOI: 10.1177/0300060520976474
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2082422-1
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  • 10
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    Lymphocytic myocarditis presenting as acute myocardial infarction: a case report and review of the literature
    SAGE Publications ; 2022
    In:  Journal of International Medical Research Vol. 50, No. 6 ( 2022-06), p. 030006052211089-
    Details
    In: Journal of International Medical Research, SAGE Publications, Vol. 50, No. 6 ( 2022-06), p. 030006052211089-
    Abstract: We report a case that presented as acute myocardial infarction (AMI) caused by lymphocytic myocarditis (LM), and explore the relationship between AMI and LM. We also performed a literature search to identify publications that previously reported LM-associated myocardial infarction. Coronary angiography of our patient revealed normal coronary arteries. However, a perfusion–metabolism mismatch in the apex and mid-inferior walls supported the diagnosis of AMI, and right ventricular septal endomyocardial biopsy showed LM. Extensive viral serological tests were negative for an infectious etiology. Immunosuppressive therapy may be beneficial in patients with high-risk myocarditis who are pathologically confirmed to be virus-negative.
    Type of Medium: Online Resource
    ISSN: 0300-0605 , 1473-2300
    URL: Article
    DOI: 10.1177/03000605221108933
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2082422-1
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