In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 2 ( 2021-2-8), p. e0246839-
Abstract:
Exclusive breastfeeding promotes beneficial modifications on the microbiota of cesarean born infants, but little is known about the role of specific breast milk components in this modulation. Women with an active FUT2 gene (called secretors ) secrete α1–2 fucosylated human milk oligosaccharides (HMOs), which promote Bifidobacterium in the infant’s gut and may modulate the microbiota of cesarean born infants. Objective To compare the microbiota composition of cesarean and vaginally born infants breastfed by secretor mothers. Methods Maternal secretor status was determined by the occurrence of 4 different α1–2 fucosylated HMOs in breast milk by LC-MS. The fecal microbiota composition from cesarean and vaginally born infants was analyzed by 16S rRNA gene sequencing and qPCR, stratified by the maternal secretor status, and compared. Results Alpha and beta diversity were not significantly different in cesarean born, secretor-fed infants (CSe+) compared to vaginally born, secretor-fed infants (VSe+). There were no significant differences in the fecal relative abundance of Bifidobacterium between CSe+ and VSe+ infants, but the prevalence of the species B . longum was lower in CSe+. The fecal relative abundance of Bacteroides was also lower, while Akkermansia and Kluyvera were higher in CSe+ infants. Conclusion Cesarean and vaginally born infants fed with breast milk containing the α1–2 fucosylated HMOs fraction present similar amounts of Bifidobacterium in the feces, but differences are observed in other members of the microbiota.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0246839
DOI:
10.1371/journal.pone.0246839.g001
DOI:
10.1371/journal.pone.0246839.g002
DOI:
10.1371/journal.pone.0246839.g003
DOI:
10.1371/journal.pone.0246839.t001
DOI:
10.1371/journal.pone.0246839.t002
DOI:
10.1371/journal.pone.0246839.t003
DOI:
10.1371/journal.pone.0246839.t004
DOI:
10.1371/journal.pone.0246839.s001
DOI:
10.1371/journal.pone.0246839.s002
DOI:
10.1371/journal.pone.0246839.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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