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  • 1
    Publication Date: 2016-02-06
    Description: Mutations in leucine-rich repeat kinase 2 (LRRK2) cause autosomal-dominant Parkinsonism with pleomorphic pathology including deposits of aggregated protein and neuronal degeneration. The pathogenesis of LRRK2-linked Parkinson's disease (PD) is not fully understood. Here, using co-immunoprecipitation, we found that LRRK2 interacted with synphilin-1 (SP1), a cytoplasmic protein that interacts with α-synuclein and has implications in PD pathogenesis. LRRK2 interacted with the N-terminus of SP1 whereas SP1 predominantly interacted with the C-terminus of LRRK2, including kinase domain. Co-expression of SP1 with LRRK2 increased LRRK2-induced cytoplasmic aggregation in cultured cells. Moreover, SP1 also attenuated mutant LRRK2-induced toxicity and reduced LRRK2 kinase activity in cultured cells. Knockdown of SP1 by siRNA enhanced LRRK2 neuronal toxicity. In vivo Drosophila studies, co-expression of SP1 and mutant G2019S-LRRK2 in double transgenic Drosophila increased survival and improved locomotor activity. Expression of SP1 protects against G2019S-LRRK2-induced dopamine neuron loss and reduced LRRK2 phosphorylation in double transgenic fly brains. Our findings demonstrate that SP1 attenuates mutant LRRK2-induced PD-like phenotypes and plays a neural protective role.
    Print ISSN: 0964-6906
    Electronic ISSN: 1460-2083
    Topics: Biology , Medicine
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  • 2
    Publication Date: 2016-12-06
    Description: Aims The effects of clouds are now recognized as critically important to the understanding of climate change impacts on ecosystems. Regardless, few studies have focused specifically on the ecophysiological responses of plants to clouds. Most continental mountain ranges are characterized by common convective cloud formation in the afternoons, yet little is known regarding this influence on plant water and carbon relations. Here we compare the ecophysiology of two contrasting, yet ubiquitous growth forms, needle-leaf and broadleaf, under representative cloud regimes of the Snowy Range, Medicine Bow Mountains, southeastern Wyoming, USA. Methods Photosynthetic gas exchange, water use efficiency, xylem water potentials and micrometeorological data were measured on representative clear, overcast and partly cloudy days during the summers of 2012 and 2013 for two indigenous broadleaf ( Caltha leptosepala and Arnica parryi ) and two needle-leaf species ( Picea engelmannii and Abies lasiocarpa ) that co-occur contiguously. Important Findings Reductions in sunlight with cloud cover resulted in more dramatic declines in photosynthesis for the two broadleaf species (ca. 50–70% reduction) versus the two conifers (no significant difference). In addition, the presence of clouds corresponded with lower leaf conductance, transpiration and plant water status in all species. However, the more constant photosynthesis in conifers under all cloud conditions, coupled with reduced transpiration, resulted in greater water use efficiency (ca. 25% higher) than the broadleaf species. These differences appear to implicate the potential importance of natural cloud patterns in the adaptive ecophysiology of these two contrasting, but common, plant growth forms.
    Print ISSN: 1752-993X
    Electronic ISSN: 1752-9921
    Topics: Biology
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  • 3
    Publication Date: 2012-09-21
    Description: In estimates of illness severity from the spring wave of the 2009 influenza A (H1N1) pandemic, reported case fatality proportions were less than 0.05%. In prior pandemics, subsequent waves of illness were associated with higher mortality. The authors evaluated the burden of the pandemic H1N1 (pH1N1) outbreak in metropolitan Atlanta, Georgia, in the fall of 2009, when increased influenza activity heralded the second wave of the pandemic in the United States. Using data from a community survey, existing surveillance systems, public health laboratories, and local hospitals, they estimated numbers of pH1N1-associated illnesses, emergency department (ED) visits, hospitalizations, intensive care unit (ICU) admissions, and deaths occurring in metropolitan Atlanta during the period August 16, 2009–September 26, 2009. The authors estimated 132,140 pediatric and 132,110 adult symptomatic cases of pH1N1 in metropolitan Atlanta during the investigation time frame. Among children, these cases were associated with 4,560 ED visits, 190 hospitalizations, 51 ICU admissions, and 4 deaths. Among adults, they were associated with 1,130 ED visits, 590 hospitalizations, 140 ICU admissions, and 63 deaths. The combined symptomatic case hospitalization proportion, case ICU admission proportion, and case fatality proportion were 0.281%, 0.069%, and 0.024%, respectively. Influenza burden can be estimated using existing data and local surveys. The increased severity reported for subsequent waves in past pandemics was not evident in this investigation. Nevertheless, the second pH1N1 pandemic wave led to substantial numbers of ED visits, hospitalizations, and deaths in metropolitan Atlanta.
    Print ISSN: 0002-9262
    Electronic ISSN: 1476-6256
    Topics: Medicine
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  • 4
    Publication Date: 2012-10-31
    Description: The taper equations used by the Ministry of Forests, Lands and Natural Resource Operations in British Columbia (BC), Canada, date back to the mid-1950s. Very little work has been done on examining the effect of climate on taper, particularly for BC but elsewhere as well. The objective of our research was to determine whether climate has an effect on tree taper for lodgepole pine ( Pinus contorta Dougl. ex Loud.) in BC. The data for this project consisted of multiple diameter inside bark measurements along the stems of 270 trees across eight biogeoclimatic zones. In addition, 20 climate variables for the sample sites were predicted from the ClimateWNA model. Kozak's variable-exponent taper model was refitted with the climate variables in the exponent of the model. The single temperature- and precipitation-related variables that provided the best fit were incorporated into the final taper model. The model was analysed as a mixed-effects model, with spatial correlation and heteroscedastic errors being explicitly modelled. Mean annual precipitation and the Julian date of the first frost after the summer growing period were the best predictors of taper. Further work is required to understand why these variables are important predictors of taper, but a possible linkage is through the tree's crown.
    Print ISSN: 0015-752X
    Electronic ISSN: 1464-3626
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 5
    Publication Date: 2013-09-28
    Description: Background: Two population surveys were conducted in Belarus: The Living Conditions, Lifestyle and Health (LLH) in 2001 ( n = 2000) and The Health in Times of Transition (HITT) in 2010 ( n = 1800). Each survey included a question on health status. The LLH questionnaire provided a 4-point Verbal Response Scale, but the HITT questionnaire used a 5-point scale. When translated into Russian, only two response categories of these scales had identical wording. These differences made a direct comparison of self-reported health status between 2001 and 2010 difficult. Methods: We conducted a Health Category Response Scale (HCRS) survey in 2010 ( n = 570) using a 100ths graduated Visual Analogue Scale (VAS) to understand how the response categories of different scales are perceived by Russian speakers. We implemented the HCRS survey’s data to calculate the weighted health status (WHS) for each of the original surveys and to compare health status in Belarus between 2001and 2010. Results: The WHS in Belarus showed a small, but statistically significant, improvement of 2.9 points on a 0–100 scale between 2001 and 2010 (56.2 vs. 59.1). Identical response categories were perceived differently on a 4-point and 5-point VAS. The category ‘good’ (‘opoee’) measured ~12 points higher, and the category ‘bad/poor’ (‘ooe’) measured ~16 points lower, on the 4-point compared with the 5-point VAS. Conclusion: Our HCRS survey and novel method enabled a direct comparison of questions with different response options. When applied to the LLH and HITT projects, we concluded that health status in Belarus has improved between 2001 and 2010.
    Print ISSN: 1101-1262
    Electronic ISSN: 1464-360X
    Topics: Medicine
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  • 6
    Publication Date: 2014-02-13
    Description: Improving the accuracy of the marine gravity field requires both improved altimeter range precision and dense track coverage. After a hiatus of more than 15 yr, a wealth of suitable data is now available from the CryoSat-2, Envisat and Jason-1 satellites. The range precision of these data is significantly improved with respect to the conventional techniques used in operational oceanography by retracking the altimeter waveforms using an algorithm that is optimized for the recovery of the short-wavelength geodetic signal. We caution that this new approach, which provides optimal range precision, may introduce large-scale errors that would be unacceptable for other applications. In addition, CryoSat-2 has a new synthetic aperture radar (SAR) mode that should result in higher range precision. For this new mode we derived a simple, but approximate, analytic model for the shape of the SAR waveform that could be used in an iterative least-squares algorithm for estimating range. For the conventional waveforms, we demonstrate that a two-step retracking algorithm that was originally designed for data from prior missions (ERS-1 and Geosat) also improves precision on all three of the new satellites by about a factor of 1.5. The improved range precision and dense coverage from CryoSat-2, Envisat and Jason-1 should lead to a significant increase in the accuracy of the marine gravity field.
    Print ISSN: 0956-540X
    Electronic ISSN: 1365-246X
    Topics: Geosciences
    Published by Oxford University Press on behalf of The Deutsche Geophysikalische Gesellschaft (DGG) and the Royal Astronomical Society (RAS).
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  • 7
    Publication Date: 2014-01-23
    Description: The first finite-dimensional parameterization of a subset of the phase space of the Navier–Stokes equations is presented. Travelling waves in two-dimensional plane Poiseuille flow are numerically shown to approximate maximum-entropy configurations. In a coordinate system moving with the phase velocity, the enclosed body of the flow exhibits a hyperbolic sinusoidal relationship between the vorticity and stream function. The phase velocity and two-amplitude parameters describe the stable manifold on the slow viscous time scale. This original parameterization provides a valuable visualization of this subset of the phase space of the Navier–Stokes equations. These new results provide physical insight into an important intermediate stage in the instability process of plane Poiseuille flow.
    Print ISSN: 0272-4960
    Electronic ISSN: 1464-3634
    Topics: Mathematics
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  • 8
    Publication Date: 2011-12-08
    Description: Smith, W. K., and Solow, A. R. 2012. Missing and presumed lost: extinction in the ocean and its inference. – ICES Journal of Marine Science, 69: 89–94. The number of modern extinctions in the ocean is unknown. The actual demise of the last individual of a species is essentially unobservable, so extinction can only be inferred. Statistical methods are described for inferring extinction from sighting records, species–area considerations, and taxonomic samples collected at two different times. The methods are illustrated using a variety of real datasets, including a sighting record of the Caribbean monk seal and results from three surveys of benthic invertebrates.
    Print ISSN: 1054-3139
    Electronic ISSN: 1095-9289
    Topics: Biology , Geosciences , Physics
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  • 9
    Publication Date: 2012-08-30
    Description: Background Longitudinal cohort studies in sub-Saharan Africa are urgently needed to understand cardiovascular disease development. We, therefore, explored health behaviours and conventional risk factors of African individuals with optimal blood pressure (BP) (≤120/80 mm Hg), and their 5-year prediction for the development of hypertension. Methods The Prospective Urban Rural Epidemiology study in the North West Province, South Africa, started in 2005 and included African volunteers ( n = 1994; aged 〉 30 years) from a sample of 6000 randomly selected households in rural and urban areas. Results At baseline, 48% of the participants were hypertensive (≥140/90 mmHg). Those with optimal BP ( n = 478) were followed at a success rate of 70% for 5 years (213 normotensive, 68 hypertensive, 57 deceased). Africans that became hypertensive smoked more than the normotensive individuals (68.2% vs 49.8%), and they also had a greater waist circumference [ratio of geometric means of 0.94 cm (95% CI: 0.86–0.99)] and greater amount of -glutamyltransferase [0.74 U/l (95% CI: 0.62–0.88)] at baseline. The 5-year change in BP was independently explained by baseline -glutamyltransferase [R 2 = 0.23, β = 0.13 U/l (95% CI: 0.01–0.19)]. Alcohol intake also predicted central systolic BP and carotid cross-sectional wall area (CSWA) at follow-up. Waist circumference was another predictor of BP changes [β = 0.18 cm (95% CI: 0.05–0.24)] and CSWA. HIV infection was inversely associated with increased BP. Conclusions During the 5 years, 24% of Africans with optimal BP developed hypertension. The surge in hypertension in Africa is largely explained by modifiable risk factors. Public health strategies should focus aggressively on lifestyle to prevent a catastrophic burden on the national health system.
    Print ISSN: 0300-5771
    Electronic ISSN: 1464-3685
    Topics: Medicine
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  • 10
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    Oxford University Press
    Publication Date: 2013-03-21
    Print ISSN: 0829-318X
    Electronic ISSN: 1758-4469
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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