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  • 1
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 19 ( 2021-09-29), p. 10534-
    Abstract: The characterization of aortic valve interstitial cells (VICs) cultured under optimal conditions is essential for understanding the molecular mechanisms underlying aortic valve stenosis. Here, we propose 2% hypoxia as an optimum VIC culture condition. Leaflets harvested from patients with aortic valve regurgitation were digested using collagenase and VICs were cultured under the 2% hypoxic condition. A significant increase in VIC growth was observed in 2% hypoxia (hypo-VICs), compared to normoxia (normo-VICs). RNA-sequencing revealed that downregulation of oxidative stress-marker genes (such as superoxide dismutase) and upregulation of cell cycle accelerators (such as cyclins) occurred in hypo-VICs. Accumulation of reactive oxygen species was observed in normo-VICs, indicating that low oxygen tension can avoid oxidative stress with cell-cycle arrest. Further mRNA quantifications revealed significant upregulation of several mesenchymal and hematopoietic progenitor markers, including CD34, in hypo-VICs. The stemness of hypo-VICs was confirmed using osteoblast differentiation assays, indicating that hypoxic culture is beneficial for maintaining growth and stemness, as well as for avoiding senescence via oxidative stress. The availability of hypoxic culture was also demonstrated in the molecular screening using proteomics. Therefore, hypoxic culture can be helpful for the identification of therapeutic targets and the evaluation of VIC molecular functions in vitro.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    MDPI AG ; 2023
    In:  Land Vol. 12, No. 2 ( 2023-02-20), p. 519-
    In: Land, MDPI AG, Vol. 12, No. 2 ( 2023-02-20), p. 519-
    Abstract: Many irrigation ponds in Fukushima Prefecture were decontaminated due to the contamination of radiocesium released from Fukushima Daiichi Nuclear Power Plant. To evaluate the impact of decontamination on 137Cs dynamics in an urban pond in Koriyama City, Fukushima Prefecture, Japan, temporal changes in 137Cs concentrations in bottom sediments and pond water were investigated before and after bottom-sediment removal. Post-decontamination, 137Cs inventories in bottom sediments decreased by 46–89%. 137Cs inventories in bottom sediments were relatively high in fine sediments before decontamination, and were also high at points near the water inlet after decontamination. Following decontamination, the mean 137Cs concentration in suspended solids (SS) and the mean dissolved 137Cs concentration in pond water decreased by 52% and 5%, respectively. Even after decontamination, the normalized 137Cs concentrations in SS and in water, which were calculated by dividing the 137Cs concentrations by the mean 137Cs inventories in each area, were higher than those in rivers, dam reservoirs, and ponds elsewhere in Fukushima. The positive correlations between δ15N values, an indicator of the source contribution to bottom sediments, and 137Cs concentrations in the upper 5 cm of bottom sediments after decontamination implied that SS from urban areas gradually increased the 137Cs inventories in the pond. The results underline the importance of secondary inputs of 137Cs from highly urbanized catchments.
    Type of Medium: Online Resource
    ISSN: 2073-445X
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2682955-1
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  • 3
    In: Land, MDPI AG, Vol. 11, No. 1 ( 2021-12-24), p. 29-
    Abstract: Given the importance of understanding long-term dynamics of radionuclides in the environment in general, and major gaps in the knowledge of 137Cs particulate forms in Chernobyl exclusion zone water bodies, three heavily contaminated water bodies (Lakes Glubokoe, Azbuchin, and Chernobyl NPP Cooling Pond) were studied to reconstruct time changes in particulate concentrations of 137Cs and its apparent distribution coefficient Kd, based on 137Cs depth distributions in bottom sediments. Bottom sediment cores collected from deep-water sites of the above water bodies were sliced into 2 cm layers to obtain 137Cs vertical profile. Assuming negligible sediment mixing and allowing for 137Cs strong binding to sediment, each layer of the core was attributed to a specific year of profile formation. Using this method, temporal trends for particulate 137Cs concentrations in the studied water bodies were derived for the first time and they were generally consistent with the semiempirical diffusional model. Based on the back-calculated particulate 137Cs concentrations, and the available long-term monitoring data for dissolved 137Cs, the dynamics of 137Cs solid–liquid distribution were reconstructed. Importantly, just a single sediment core collected from a lake or pond many years after a nuclear accident seems to be sufficient to retrieve long-term dynamics of contamination.
    Type of Medium: Online Resource
    ISSN: 2073-445X
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2682955-1
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