In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2022-7-27)
Kurzfassung:
Severe acute respiratory syndrome-related coronavirus (SARS-CoV-2) transmission occurs even among fully vaccinated individuals; thus, prompt identification of infected patients is central to control viral circulation. Antigen rapid diagnostic tests (Ag-RDTs) are highly specific, but sensitivity is variable. Discordant RT-qPCR vs. Ag-RDT results are reported, raising the question of whether negative Ag-RDT in positive RT-qPCR samples could imply the absence of infectious viruses. To study the relationship between negative Ag-RDT results with virological, molecular, and serological parameters, we selected a cross-sectional and a follow-up dataset and analyzed virus culture, subgenomic RNA quantification, and sequencing to determine infectious viruses and mutations. We demonstrated that RT-qPCR positive while SARS-CoV-2 Ag-RDT negative discordant results correlate with the absence of infectious virus in nasopharyngeal samples. A decrease in sgRNA detection together with an expected increase in detectable anti-S and anti-N IgGs was also verified in these samples. The data clearly demonstrate that a negative Ag-RDT sample is less likely to harbor infectious SARS-CoV-2 and, consequently, has a lower transmissible potential.
Materialart:
Online-Ressource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2022.912138
DOI:
10.3389/fmicb.2022.912138.s001
DOI:
10.3389/fmicb.2022.912138.s002
DOI:
10.3389/fmicb.2022.912138.s003
DOI:
10.3389/fmicb.2022.912138.s004
DOI:
10.3389/fmicb.2022.912138.s005
DOI:
10.3389/fmicb.2022.912138.s006
DOI:
10.3389/fmicb.2022.912138.s007
DOI:
10.3389/fmicb.2022.912138.s008
Sprache:
Unbekannt
Verlag:
Frontiers Media SA
Publikationsdatum:
2022
ZDB Id:
2587354-4
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