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  • Frontiers Media SA  (15)
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  • Frontiers Media SA  (15)
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  • 1
    Online Resource
    Online Resource
    Large-Scale Cultivation of Spirulina for Biological CO2 Mitigation in Open Raceway Ponds Using Purified CO2 From a Coal Chemical Flue Gas
    Frontiers Media SA ; 2020
    In:  Frontiers in Bioengineering and Biotechnology Vol. 7 ( 2020-1-9)
    Details
    In: Frontiers in Bioengineering and Biotechnology, Frontiers Media SA, Vol. 7 ( 2020-1-9)
    Type of Medium: Online Resource
    ISSN: 2296-4185
    URL: Article
    DOI: 10.3389/fbioe.2019.00441
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2020
    detail.hit.zdb_id: 2719493-0
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  • 2
    Online Resource
    Online Resource
    Understanding farmers’ eco-friendly fertilization technology adoption behavior using an integrated S-O-R model: The case of soil testing and formulated fertilization technology in shaanxi, china
    Frontiers Media SA ; 2022
    In:  Frontiers in Environmental Science Vol. 10 ( 2022-9-27)
    Details
    In: Frontiers in Environmental Science, Frontiers Media SA, Vol. 10 ( 2022-9-27)
    Abstract: The rapid development of high-standard farmland construction has triggered explosion of farmland cultivated land protection technologies in recent years. Contemporary, eco-friendly fertilization technology (EFFT) are rapidly becoming main force to achieve food security and promote the national ecological strategy. Hence, based on the extended stimulus-organism-response (S-O-R) theoretical analysis framework, this empirical research 295 field interview data of farmers in the high standard farmland grain main production area in Shaanxi Province, a developing country context, to analyze the mechanism of external incentives on farmers’ eco-friendly fertilization technology adoption behavior, further tested to examine the mediating effect of intrinsic perception and moderating effect of the family endowment. We applied the methodological approach, partial least square structural equation modeling (PLS-SEM), to test the hypothetical model. The results show that external incentives can effectively improve farmers’ technology adoptive behavior; internal perception has a significant positive effect on adoptive behavior, and it plays an intermediary role between external incentives and eco-friendly fertilization technology adoption behavior; family endowment has a significant positive effect on farmers’ technology adoption behavior, but the moderating effect of family endowment in external incentive-technology adoption behavior relationship is not significant. Therefore, we should choose appropriate and flexible government regulations, and give full play to the role of premium incentives, so as to improve the motivation of farmers to adopt eco-friendly fertilization technology.
    Type of Medium: Online Resource
    ISSN: 2296-665X
    URL: Article
    DOI: 10.3389/fenvs.2022.991255
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2741535-1
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  • 3
    Online Resource
    Online Resource
    Comprehensive evaluation of growth characteristics, nitrogen removal capacity, and nutritional properties of three diet microalgae
    Frontiers Media SA ; 2023
    In:  Frontiers in Marine Science Vol. 10 ( 2023-2-3)
    Details
    In: Frontiers in Marine Science, Frontiers Media SA, Vol. 10 ( 2023-2-3)
    Abstract: Nitrogen is one of the main pollution sources in aquaculture system. Microalgae are considered as one of the ideal bio-absorbents used in wastewater purification, due to their nitrogen removal capacity and more importantly nutritional value. Nannochloropsis oceanica , Cyclotella atomus , and Conticribra weissflogii are famous as diet microalgae. However, estimation of nitrogen removal capability and concomitant nutritional properties of the three species have been rarely reported, which was performed in this study. N. oceanica , C. atomus , and C. weissflogii were cultivated with two initial nitrate-N concentration, noted as NC (13.85 mg·L –1 ) and NW (5 mg·L –1 ) groups, respectively. All the three microalgal strains in NC group showed higher maximum cell density, specific growth rate, and biomass concentration, maximal quantum yield of PSII (Fv/Fm), total Chlorophyll and carotenoids contents than that in NW group. These results confirmed the importance of nitrogen for microalgal biomass generation and photosynthetic performance. From Fv/Fm, N. oceanica has better adaptability towards nitrogen depletion compared to other two selected strains. The three microalgae exhibited significantly stronger nitrate-N absorption efficiencies in NC group at the same timepoint, compared to NW. Analyzing the average amount of nitrate-N absorbed by each cell daily, C. weissflogii gained the largest value, followed successively by C. atomus and N. oceanica , likely due to species specificity. Moreover, three strains removed 90% of nitrate-N within five days and 99% of that within seven days, showing splendid nitrogen removal potentials. These results confirmed the feasibility of removing nitrogen from wastewater with the selected three microalgae. Nutritional properties of microalgal biomass were also investigated. For the three species, lower nitrate-N was beneficial for the production of soluble sugar, total lipid, and saturated fatty acid, while higher nitrate-N led to more soluble protein and polyunsaturated fatty acid. In summary, N. oceanica , C. atomus , and C. weissflogii all showed strong nitrogen removal capacity, whose growth characteristics and nutritional properties varied with nitrogen concentration. In practical application for assimilating nitrogen, these findings could provide some references for the selection of suitable microalgae species in order to satisfy different nutritional requirements of various aquatic animals.
    Type of Medium: Online Resource
    ISSN: 2296-7745
    URL: Article
    DOI: 10.3389/fmars.2023.1117043
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2757748-X
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  • 4
    Online Resource
    Online Resource
    Table1.pdf
    Frontiers Media SA ; 2022
    In:  Frontiers in Environmental Science Vol. 10 ( 2022-6-30)
    Details
    In: Frontiers in Environmental Science, Frontiers Media SA, Vol. 10 ( 2022-6-30)
    Abstract: With the development of the economy and society, environmental pollution and resource waste problems are emerging, especially in agricultural production, and the adoption of ecological agricultural technologies is a prerequisite to alleviate ecological pressure. Based on the Technology Acceptance Model—Theory of Planned Behavior (TAM-TPB) and using research data from Hubei, Hunan, and Anhui provinces, this paper empirically analyzes the factors influencing farmers’ intention to adopt rice and shrimp crop technologies using the PLS-SEM method. The configuration path of high technology intention was further investigated by the fsQCA method. The results showed that: 1) farmers’ intention to adopt rice-shrimp crop technology was mainly positively influenced by behavioral attitude, subjective norm, perceived behavioral control, behavioral attitude; 2) Perceived usefulness and perceived ease of use had a direct effect on farmers’ intention to adopt and an indirect effect with behavioral attitude as a mediating variable, while perceived ease of use had a positive effect and perceived usefulness did not. In doing so, four configuration paths of high technology acceptance intention were obtained. Given this, this paper makes relevant suggestions, suggesting that the relevant departments focus on the comprehensive benefits of rice-shrimp crop technology; agricultural technology departments provide technical assistance to farmers, and village committees organize regular inter-farmer exchanges.
    Type of Medium: Online Resource
    ISSN: 2296-665X
    URL: Article
    URL: Article
    DOI: 10.3389/fenvs.2022.927668
    DOI: 10.3389/fenvs.2022.927668.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2741535-1
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  • 5
    Online Resource
    Online Resource
    Screening of antibiotics to obtain axenic cell cultures of a marine microalga Chrysotila roscoffensis
    Frontiers Media SA ; 2023
    In:  Frontiers in Bioengineering and Biotechnology Vol. 11 ( 2023-5-25)
    Details
    In: Frontiers in Bioengineering and Biotechnology, Frontiers Media SA, Vol. 11 ( 2023-5-25)
    Abstract: Due to high growth rate, outstanding abiotic stress tolerance, and rich value-added substances, Chrysotila roscoffensis , belonging to the phylum of Haptophyta, can be considered as a versatile resource for industrial exploitation of bioactive compounds. However, the application potential of C. roscoffensis has drawn attention until just recently, and the understanding related to the biological properties of this species is still scarce. For example, the sensitivities of C. roscoffensis to antibiotics, which is essential for the verification of heterotrophic capacity and the establishment of efficient genetic manipulation system is still unavailable. Aiming to provide fundamental information for future exploitation, the sensitivities of C. roscoffensis to nine types of antibiotics were tested in this study. The results demonstrated that C. roscoffensis exhibited relatively high resistances to ampicillin, kanamycin, streptomycin, gentamicin, and geneticin, while was sensitive to bleomycin, hygromycin B, paromomycin, and chloramphenicol. Using the former five types of antibiotics, a bacteria removal strategy was established tentatively. Finally, the axenicity of treated C. roscoffensis was confirmed based on a multi-strategy method including solid plate, 16S rDNA amplification, and nuclear acid staining. This report can provide valuable information for the development of optimal selection markers, which are meaningful for more extensive transgenic studies in C. roscoffensis . Moreover, our study also paves the way for the establishment of heterotrophic/mixotrophic cultivation modes of C. roscoffensis .
    Type of Medium: Online Resource
    ISSN: 2296-4185
    URL: Article
    DOI: 10.3389/fbioe.2023.1218031
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2719493-0
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  • 6
    Online Resource
    Online Resource
    Research on the Influence Mechanism of Agricultural Entrepreneurship: Evidence From Five Provinces in Western China
    Frontiers Media SA ; 2022
    In:  Frontiers in Psychology Vol. 13 ( 2022-6-1)
    Details
    In: Frontiers in Psychology, Frontiers Media SA, Vol. 13 ( 2022-6-1)
    Abstract: Individual entrepreneurial behaviors will be affected by their attitudes and environmental factors. Therefore, entrepreneurial attitude and entrepreneurial environment interpret the entrepreneurial behavior mechanism of farmers from the perspectives of internal and external factors. This manuscript is based on a survey data of farmers in five western provinces in China. Using structural equation modeling, the mechanism of the effects of farmers’ entrepreneurial attitudes and entrepreneurial environment on entrepreneurial behavior was analyzed empirically. The research results show that individual entrepreneurial attitudes and the external entrepreneurial environment cannot directly affect entrepreneurial behavior, and both will be through entrepreneurial self-efficacy. The sense of entrepreneurship indirectly affects entrepreneurial behavior, and entrepreneurial self-efficacy plays an intermediary role. The “entrepreneurial behavior structure” model proposed in this manuscript points out that cultivating entrepreneurial attitudes and creating an entrepreneurial environment cannot directly promote entrepreneurial behavior of farmers, but must stimulate farmers’ entrepreneurial behavior. The sense of self-efficacy provides a direction for the promotion of policy formulation and practical guidance for farmers’ entrepreneurship.
    Type of Medium: Online Resource
    ISSN: 1664-1078
    URL: Article
    DOI: 10.3389/fpsyg.2022.864226
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2563826-9
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  • 7
    Online Resource
    Online Resource
    Analysis of spatio-temporal changes and driving forces of cultivated land in China from 1996 to 2019
    Frontiers Media SA ; 2022
    In:  Frontiers in Environmental Science Vol. 10 ( 2022-10-28)
    Details
    In: Frontiers in Environmental Science, Frontiers Media SA, Vol. 10 ( 2022-10-28)
    Abstract: Cultivated land is an important prerequisite and guarantee for food production and security, and the change of cultivated land resources in China has always been concerned. National land survey is an effective way to accurately grasp the area and distribution of cultivated land resources. However, due to the differences in technical means and statistical standards at different stages, there are obvious breakpoints among the cultivated land area data of the three land surveys in China, which hinders the in-depth study of the spatio-temporal distribution of cultivated land resources in long-time series. The Autoregressive Integrated Moving Average model is used to reconstruct and mine the cultivated land area data from 1996 to 2019 based on the data of the third land survey in China. The spatio-temporal variation characteristics of cultivated land area are explored by using Geographic Information System spatial analysis, and the driving factors of cultivated land change are analyzed based on Geographical Detector (GeoDetector) from the perspective of social, economic, agricultural and natural. The results show that the area of cultivated land in China decreased continuously from 1996 to 2019, with a sharp decrease from 1996 to 2004 and a slow decrease from 2005 to 2019. From 1996 to 2019, there were obvious spatial differences in the change of cultivated land area in 31 provincial units. From 1996 to 2008, the cultivated land area in 29 provinces showed a downward trend, especially in the central and northern regions such as Shaanxi, Sichuan and Inner Mongolia. From 2008 to 2019, the cultivated land area in the underdeveloped areas of Heilongjiang, Jilin, Liaoning, Xinjiang, Gansu and Tibet increased significantly, while the rest showed a downward trend. Factor detection found that the q values of population, regional gross domestic product grain output, the proportion of the added value of the primary industry and average slope were all more than 0.5, which had an important impact on the change of cultivated land area. The explanatory power of the interaction between factors on the change of cultivated land area is enhanced in different degrees compared with the single factor effect, which is manifested in the enhancement of bilinear or nonlinear enhancement, and the interaction of different factors promotes the change of cultivated land area. The change of cultivated land area is the result of complex interaction between factors, and is closely related to the land policy in the same period.
    Type of Medium: Online Resource
    ISSN: 2296-665X
    URL: Article
    DOI: 10.3389/fenvs.2022.983289
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2741535-1
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  • 8
    Online Resource
    Online Resource
    Data_Sheet_1.docx
    Frontiers Media SA ; 2022
    In:  Frontiers in Microbiology Vol. 13 ( 2022-8-31)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2022-8-31)
    Abstract: Nannochloropsis has been considered as a promising feedstock for the industrial production of violaxanthin. However, a rational breeding strategy for the enhancement of violaxanthin content in this microalga is still vacant, thereby limiting its industrial application. All- trans -lycopene locates in the first branch point of carotenogenesis. The carotenoid isomerase (CRTISO), catalyzing the lycopene formation, is thus regarded as a key enzyme for carotenogenesis. Phaeodactylum tricornutum can accumulate high-level carotenoids under optimal conditions. Therefore, it is feasible to improve violaxanthin level in Nannochloropsis by overexpression of PtCRTISO . Protein targeting analysis of seven PtCRTISO candidates (PtCRTISO1–6 and PtCRTISO-like) demonstrated that PtCRTISO4 was most likely the carotenoid isomerase of P . tricornutum . Moreover, the transcriptional pattern of PtCRTISO4 at different cultivation periods was quite similar to other known carotenogenesis genes. Thus, PtCRTISO4 was transformed into N . oceanica . Compared to the wild type (WT), all three transgenic lines (T1–T3) of N . oceanica exhibited higher levels of total carotenoid and violaxanthin. Notably, T3 exhibited the peak violaxanthin content of 4.48 mg g –1 dry cell weight (DCW), which was 1.68-folds higher than WT. Interestingly, qRT-polymerase chain reaction (PCR) results demonstrated that phytoene synthase ( NoPSY ) rather than ζ-carotene desaturase ( NoZDS ) and lycopene β-cyclase ( NoLCYB ) exhibited the highest upregulation, suggesting that PtCRTISO4 played an additional regulatory role in terms of carotenoid accumulation. Moreover, PtCRTISO4 overexpression increased C18:1n-9 but decreased C16:1n-7, implying that C18:1 may serve as a main feedstock for xanthophyll esterification in Nannochloropsis . Our results will provide valuable information for the violaxanthin production from Nannochloropsis .
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2022.942883
    DOI: 10.3389/fmicb.2022.942883.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2587354-4
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  • 9
    Online Resource
    Online Resource
    Effect of high glucose supplementation on pulmonary fibrosis involving reactive oxygen species and TGF-β
    Frontiers Media SA ; 2022
    In:  Frontiers in Nutrition Vol. 9 ( 2022-10-11)
    Details
    In: Frontiers in Nutrition, Frontiers Media SA, Vol. 9 ( 2022-10-11)
    Abstract: This study explored the profibrotic impact of high glucose in the lung and potential mechanisms using latent TGF-β1-induced human epithelial cell pulmonary fibrosis and bleomycin (BLM)-induced pulmonary fibrosis models. Results demonstrated that high glucose administration induced epithelial–mesenchymal transition (EMT) in human epithelial cells in a dose-dependent manner via activating latent TGF-β1, followed by increased expression of mesenchymal-related proteins and decreased expression of epithelial marker protein E-cadherin. Further mechanism analysis showed that administration of high glucose dose-dependently promoted total and mitochondrial reactive oxygen species (ROS) accumulation in human epithelial cells, which promoted latent TGF-β1 activation. However, N -acetyl- L -cysteine, a ROS eliminator, inhibited such effects. An in vivo feed study found that mice given a high-glucose diet had more seriously pathological characteristics of pulmonary fibrosis in BLM-treated mice, including increasing infiltrated inflammatory cells, collagen I deposition, and the expression of mesenchymal-related proteins while decreasing the expression of the epithelial marker E-cadherin. In addition, high glucose intake further increased TGF-β1 concentration and upregulated p-Smad2/3 and snail in lung tissues from BLM-treated mice when compared to BLM-treated mice. Finally, supplementation with high glucose further increased the production of lipid peroxidation metabolite malondialdehyde and decreased superoxide dismutase activity in BLM-treated mice. Collectively, these findings illustrate that high glucose supplementation activates a form of latent TGF-β1 by promoting ROS accumulation and ultimately exacerbates the development of pulmonary fibrosis.
    Type of Medium: Online Resource
    ISSN: 2296-861X
    URL: Article
    DOI: 10.3389/fnut.2022.998662
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2776676-7
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  • 10
    Online Resource
    Online Resource
    Data_Sheet_1.docx
    Frontiers Media SA ; 2023
    In:  Frontiers in Medicine Vol. 10 ( 2023-5-25)
    Details
    In: Frontiers in Medicine, Frontiers Media SA, Vol. 10 ( 2023-5-25)
    Abstract: Ambroxol is a widely used mucoactive drug in sputum clearance of respiratory diseases taken orally and by injection. However, there is a paucity of evidence for inhaled ambroxol in sputum clearance. Methods This study performed a multicenter, randomized, double-blind, placebo-controlled, phase 3 trial at 19 centers in China. Hospitalized adult patients with mucopurulent sputum and expectoration difficulty were recruited. Patients were randomized by 1:1 to receive inhalation of either ambroxol hydrochloride solution 3 mL (22.5 mg) + 0.9% sodium chloride 3 mL or 0.9% sodium chloride 6 mL twice daily for 5 days, with an interval of more than 6 h. The primary efficacy endpoint was the absolute change in the sputum property score after treatment compared to the baseline in the intention-to-treat population. Results Between 10 April 2018 and 23 November 2020, 316 patients were recruited and assessed for eligibility, of whom 138 who received inhaled ambroxol and 134 who received a placebo were included. Patients who received inhaled ambroxol had a significantly greater decrease in the sputum property score compared with patients who received inhalation of placebo (difference: −0.29; 95% CI: −0.53 to −0.05; p  = 0.0215). Compared with the placebo, inhaled ambroxol also significantly reduced more expectoration volume in 24 h (difference: −0.18; 95% CI: −0.34 to −0.03; p   = 0.0166). There was no significant difference in the proportion of adverse events between the two groups, and no deaths were reported. Discussion In hospitalized adult patients with mucopurulent sputum and expectoration difficulty, inhaled ambroxol was safe and effective for sputum clearance compared with a placebo. Clinical trial registration [ https://www.chictr.org.cn/showproj.html?proj=184677 ], Chinese Clinical Trial Registry [ChiCTR2200066348] .
    Type of Medium: Online Resource
    ISSN: 2296-858X
    URL: Article
    URL: Article
    DOI: 10.3389/fmed.2023.1182602
    DOI: 10.3389/fmed.2023.1182602.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2775999-4
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