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Digitale Medien

Fibronectin binding by Propionibacterium acnes (1997)

Yu, Jian-Lin ; Månsson, Roland ; Flock, Jan-Ingmar ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

FEMS immunology and medical microbiology 19 (1997), S. 0

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ISSN: 1574-695X

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie , Medizin

Notizen: Strains of Propionibacterium acnes, isolated from different kinds of orthopaedic and biomaterial-associated infections and from skin flora were shown to express binding of soluble as well as immobilized fibronectin. Among these 7 strains isolated from orthopaedic infections, 2 from breast prostheses, and 9 skin isolates, 2, 2, and 5 strains respectively bound immobilized fibronectin. The fibronectin binding was sensitive to protease and heat treatment, and was inhibited by a cell surface extract from one of the binding strains. In SDS-PAGE and autoradiography of cell surface extracts, a band corresponding to a MW of about 80 kD reacted with fibronectin and the 150 kD fragment of fibronectin. Binding to fibronectin and the 150 kD fragment of fibronectin could be inhibited with heparin. We thus present a first Fn binding protein of P. acnes, a surface exposed protein of 80 kD. None of the strains bound soluble collagen, and only one strain expressed weak binding of vitronectin and bone sialoprotein II.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1574-695X.1997.tb01094.x

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Digitale Medien

Carbonic anhydrase activity and inorganic carbon fluxes in low- and high-C1 cells of Chlamydomonas reinhardtü and Scenedesmus obliquus (1994)

Palmqvist, Kristin ; Yu, Jian-Wei ; Badger, Murray R.

Oxford, UK : Blackwell Publishing Ltd

Physiologia plantarum 90 (1994), S. 0

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ISSN: 1399-3054

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie

Notizen: Carbonic anhydrase (CA) activity associated with high- and low-dissolved inorganic carbon (C1) grown cells was examined in whole cells by measuring 18O exchange from doubly labeled CO2 (13C18O18O). Both algal species showed the presence of extracellular (periplasmic) as well as intracellular CA activity, which were both greatly increased in low-C1 cells. The periplasmic CA activity was at least 40-fold higher in lowcompared to high-C1 cells in both C. reinhardtii and S. obliquus. while low-C1 cells of S. obliquus showed the highest activity of internal CA. The CA inhibitor ethoxyzolamide showed a strong inhibition of the C1 uptake process in both C. reinhardtii and S. obliquus as in cyanobacteria. which may indicate that the nature of the primary uptake process is similar in both green algae and cyanobacteria. By using a mass spectrometnc disequilibrium technique it was possible to separate the C1 fluxes of net HCO−3-uptake and net CO2-uptake during steady-state photosynthesis in high- and Sow-C1 grown cells of Chlamydomonas reinhardtii (WT. 2137+) and Scenedesmus obliquus (WT. D3). It was found that both high- and low-C1 cells of the two algae can utilize both CO2 and HCO−3 for photosynthesis, although low-C1 cells have a higher affinity for the uptake of both C1 species. Induction at low-C1 causes an increase in the affinity of both species for HCO−3 and CO2; changes in net CO2-uptake were, however, significantly greater.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb08812.x

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Digitale Medien

Measurement of CO2 and HCO3− fluxes in cyanobacteria and microalgae during steady-state photosynthesis (1994)

Badger, Murray R. ; Palmqvist, Kristin ; Yu, Jian-Wei

Oxford, UK : Blackwell Publishing Ltd

Physiologia plantarum 90 (1994), S. 0

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ISSN: 1399-3054

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie

Notizen: A mass spectrometric procedure is described which allows the simultaneous estimation of both CO2 and HCO−3 fluxes associated with cyanobacteria and green algae during steady-stale photosynthesis. This technique utilizes the chemical disequilibrium which exists between CO2 and HCO−3 during photosynthesis in cell suspensions which lack carbonic anhydrase activity. The kinetic equations which are derived for flux determinations are based on models of photosynthesis in both cyanobacteria and green algae which seem most reasonable given our present level of understanding, together with direct measurement of [CO2] estimation of [HCO−3] and application of the kinetic rate constants for the interconversion of CO2 and HCO−3 From measurements made in the light, net uptake of both CO2 and HCO−3 can be readily determined. In addition, analysis of the dark phase immediately following light-off provides the possibility of also determining the CO2 evolution whích ís occurring during photosynthesis, and thus also the gross CO2 uptake rates in the light. Results are presented for the response of dissolved inorganic carbon (C1) flux rates to external C, in low-C1 grown cells of both Synechococcus PCC7942 and Chlamydomonas reinhardtii and these are consistent with previous studies showing that such cells possess capacities to utilize both CO2 and HCO−3 for photosynthesis. The advantages and potential errors which are inherent in this technique are discussed together with its potential for future studies on C1 transport under various experimental conditions.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb08811.x

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