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  • 1
    Digitale Medien
    Digitale Medien
    Characterization of Cholecystokinin Binding Sites in Rat Cerebral Cortex Using a 125I-CCK-8 Probe Resistant to Degradation (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 40 (1983), S. 0 
    Details
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Abstract: Specific binding sites for cholecystokinin (CCK) have been characterized in a particulate membrane fraction of rat cerebral cortex using a biologically active 125I-labeled derivative of the C-terminal octapeptide of CCK (CCK-8) prepared by reaction with the iodinated form of the imidoester (125IIE), methyl-p-hydroxybenzimidate. The time course of binding to cortical membranes was rapid, temperature dependent, and saturable. Half-maximal binding at 24°C was reached in 30 min and full binding at 120 min. At 37°C there was only a slight increase in 125IIE-CCK-8 bound after 15 min. The addition of a large excess of CCK-8 after 30 min of binding at 24°C caused a prompt and rapid decline in radioligand bound showing that the interaction was reversible. There was a progressive decline in the amount of 125IIE-CCK-8 bound to membranes with increasing concentrations of CCK-8 and other structurally related peptides. CCK-8 displaced 50% of the radioligand at 4 nM, CCK-33 at 10 nM, and gastrin (desulfated CCK-8) at 60 nM. Secretin, a structurally unrelated peptide, was unable to displace the radioligand from cortical membranes at 1.0 μM. Finally, 125IIE-CCK-8 exposed to cortical membranes or to buffers that had previously contained such membranes for 60 min at 24°C bound equally as well to fresh cortical membranes as control radioligand that had not been exposed to the same conditions. Thus the 125I-CCK-8 radioligand used in this study was highly resistant to degradative processes in rat brain tissue.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1471-4159.1983.tb13583.x
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  • 2
    Digitale Medien
    Digitale Medien
    Lymphocyte predominance Hodgkin's disease (nodular paragranuloma)–a bcl-2 negative germinal centre lymphoma (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Histopathology 19 (1991), S. 0 
    Details
    ISSN: 1365-2559
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Hodin's disease, lymphocyte predominance type (nodular paragranuloma), is of germinal centre origin and the tumours cells have a B-cell phenotype. As the t(14;18) translocation, and the subsequent expression of bcl-2 protein by germinal centre cells, is the most characteristic finding of centroblastic-centrocytic lymphoma, we have tested a series of 11 cases of lymphocyte predominance Hodgkin's disease, using Southern blot analysis for the major breakpoint region and the minor breakpoint cluster region, polymerase chain reaction with primers for the major and minor breakpoint cluster region, and immunohistological studies with a monoclonal antibody specific for the bcl-2 protein. All three techniques gave negative results in the cases of Hodgkin's disease, establishing a clear differentiation from centroblastic-centrocytic lymphoma. These findings are useful in the differential diagnosis between the two entities and raise the question of the non-clonal nature of lymphocyte predominance Hodgkin's disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2559.1991.tb00896.x
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  • 3
    Digitale Medien
    Digitale Medien
    A new method for the estimation of total dissolved salts in saturation extracts of soils from electrical conductivity (1994)
    Oxford, UK : Blackwell Publishing Ltd
    European journal of soil science 45 (1994), S. 0 
    Details
    ISSN: 1365-2389
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: We have determined electrical conductivity (Ee) and total dissolved salts (S) in saturation extracts from 39 soil samples from the Baza basin (Province of Granada, south-east Spain). Ee ranged from 2.8 to 110dS m−3, and S from 2 to 444 8 dm−3.The relationship between S and Ee was not linear. When the saturation extracts were diluted with progressively larger quantities of distilled water and their electrical conductivity calculated (Eec) with the equation〈displayedItem type="mathematics" xml:id="mu1" numbered="no"〉〈mediaResource alt="image" href="urn:x-wiley:13510754:EJSS153:EJSS_153_mu1"/〉where Ed and Ew are the conductivity of the diluted extract and the distilled water and f is the dilution factor, the relationship between S and Eec tended to become linear.The highest linear correlation coefficient relating S (mg dm−3) and Eec (dS m−1) was reached when Eec, values were calculated for dilutions with a conductivity (Ed) between 0.1 and 0.3 dS m−1 (E*ec). The regression equation was S= 490 E*ec with r2= 0.999. This relationship can be used in all saturation extracts, regardless of the concentration and type of ions present.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2389.1994.tb00496.x
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  • 4
    Digitale Medien
    Digitale Medien
    rDNA RFLPs as genetic markers for resource management in brown trout (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 55 (1999), S. 0 
    Details
    ISSN: 1095-8649
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Interesting population-specific rDNA markers were detected for microgeographical analysis of brown trout Salmo trutta as well as for monitoring stocking practices.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1095-8649.1999.tb00671.x
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  • 5
    Digitale Medien
    Digitale Medien
    Low stocking incidence in brown trout populations from northwestern Spain monitored by LDH-5* diagnostic marker (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 47 (1995), S. 0 
    Details
    ISSN: 1095-8649
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Brown trout Salmo trutta is one of the most valuable species inhabiting river drainages in Galicia (northwestern Spain). The influence of man, through overfishing and pollution, is thought to have caused the decline of trout in Galician rivers. To balance the possible population decline, Galician rivers have been stocked extensively with a brown trout stock of German origin during the last 30 years. In this study, the incidence of stocking practices has been investigated by an LDH-5* genetic marker. A very low number of stocked individuals have been observed within river populations, despite the long period of repopulation. Only eight out of the 44 populations analysed showed a limited number of individuals of hatchery origin. Most stocked individuals detected were 0+ age, and showed a poor condition factor (K 〈 1). Environmental factors (muscular stamina and food habits) and genetic factors (different selective conditions in hatchery medium) are invoked to explain the low viability of hatchery fish Observed in this Study.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1095-8649.1995.tb06053.x
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  • 6
    Digitale Medien
    Digitale Medien
    Allergic contact dermatitis due to 1,2-benzisothiazolin-3-one in paint manufacture (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Contact dermatitis 27 (1992), S. 0 
    Details
    ISSN: 1600-0536
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1600-0536.1992.tb03272.x
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  • 7
    Digitale Medien
    Digitale Medien
    Analysis of European eel (Anguilla anguilla) chromosomes after treatment with TfiI and AvaI restriction endonucleases (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of applied ichthyology 14 (1998), S. 0 
    Details
    ISSN: 1439-0426
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: In this paper, we describe the banding pattern induced on fixed eel chromosomes by treatment with restriction endonucleases which recognize 5 bp (TfiI) and 6 bp (AvaI) targets. Several time treatments were tested. The principle factor implicated by the appearance of a serial G-like pattern is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0426.1998.tb00625.x
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