ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract The genes determining the biosynthesis of a new pcolonization factor, designated PCFO9 have been cloned from an LT+ enterotoxigenic Escherichia coli 09: H− isolated during an outbreak of infant diarrhea in Central Australia. Electron microscopy has shown it to be of the fibrillar type. Purification of the major pilin subunit showed it to have a size of approximately 27 kDa. NH2-terminal analysis of the major subunit has shown the PCFO9 determinant to be distinct from other fimbriae although there is some conservation of certain residues. A synthetic oligodeoxynucleotide probe based on NH2-terminal amino acid sequence of the purified protein has been used in Southern hybridization analyses to define the region on pPM1320 encoding the structural gene for the major pilin subunit.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1990.tb03972.x
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