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  • 1980-1984  (2)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    An insertion mutation associated with constitutive expression of repressible acid phosphatase in Saccharomyces cerevisiae (1983)
    Springer
    Molecular genetics and genomics 191 (1983), S. 339-346 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The PHO83 mutation in Saccharomyces cerevisiae, which had been detected on the basis of constitutive production of repressible acid phosphatase and mapped at the end of the PHO5 locus, was analysed by Southern hybridization with cloned DNA fragments of the PHO5 gene as probe. It was shown that this mutant has a DNA insertion of about 6 kilobase pairs, probably in the 5′-noncoding region of the PHO5 gene. Production of repressible acid phosphatase by the PHO83 mutant is partially independent of the function of the PHO2 and PHO4 genes, the positive regulatory genes whose functions are indispensable for PHO5 expression. PHO83 mutants are constitutive in a or α cells, either haploid or diploid, but not in non-mating cells, MATa/MATα or a certain sterile mutation. These observations strongly suggest that the PHO83 mutation is caused by insertion of a Ty element in the 5′-noncoding region of the PHO5 gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425743
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Two-spored asci produced by interrupted sporulation: A novel approach to linkage analysis in yeast (1983)
    Springer
    Molecular genetics and genomics 191 (1983), S. 165-166 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetical analysis of two-spored asci formed by interrupted sporulation offers a novel procedure for mapping of centromere-linked genes in Saccharomyces cerevisiae. Unlike the two-spored asci encountered under normal sporulation conditions, these asci are produced by a nonrandom mechanism. They fall into three categories (++), (+-) and (--) with respect to any marker. The percentage of (+-) asci varies directly as a function of centromere-linkage of a gene. It is observed that almost 100% asci are of the (+-) type in case of very tightly linked genes like trp-1 and cdc-10, while in case of markers unlinked to the centromere, e. g, trp-5 and met-8, the (+-) asci constitute 50% of the total number of asci. Other markers with varying degrees of linkage, e. g. ura-3 and lys-1 show corresponding numbers of (+-) asci between 50% and 100% of the total asci. These findings are in contrast to the results expected from a random abortion of two spores, in which case the (+-) asci would constitute 67% of the total number of asci irrespective of the degree of centromere linkage of a marker. The linkage-dependent segregation of markers in these new kind of two-spored asci permits a rapid and accurate estimate of centromere linkage of a gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330906
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    Paper (German National Licenses)
    Fulltext
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