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  • 1
    Digitale Medien
    Digitale Medien
    Production and Characterization of Monoclonal Antibodies to Peripheral and Central Nervous System Myelin (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 43 (1984), S. 0 
    Details
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Abstract: Monoclonal antibodies against P0, myelin basic protein, or myelin-associated glycoprotein were generated by fusing mouse myeloma cells with spleen cells from BALB/c mice immunized with central and peripheral nervous system myelin proteins. The antibodies secreted were either IgG, IgM, or IgA. Clone C6B5 (iso-type IgM) secreted antibody(ies) that bound to both myelin basic protein and myelin-associated glycoprotein, although binding of antibody to myelin basic protein as detected by the immunoblot technique appeared to be much less than to the myelin-associated glycoprotein. Antibodies were characterized in solid-phase radioimmunoassay for their species cross-reaction, and histologically for the specificity of binding to myelin in central and peripheral nervous system tissues. These monoclonal reagents should prove valuable in studying CSF and myelin-producing cells, since in both cases the concentration of myelin proteins is low.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1471-4159.1984.tb00914.x
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  • 2
    Digitale Medien
    Digitale Medien
    Functional consequence of variation in melanoma antigen expression (1983)
    Springer
    Cancer immunology immunotherapy 16 (1983), S. 30-34 
    Details
    ISSN: 1432-0851
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Melanoma cells have been shown to express melanoma-associated antigens and, in many cases, the histocompatibility antigen, HLA-DR. We questioned whether the expression of these antigens was quantitatively altered during the serial passage of melanoma cells in culture. Therefore, we measured the binding of monoclonal antibodies specific for a melanoma-specific antigen and the HLA-DR antigen to melanoma cells from serial passages. Three cell lines were studied. We found that although both the melanoma-associated antigen and the HLA-DR antigen were qualitatively conserved, significant quantitative differences were seen. To study the functional consequences of these differences, we used fluorescence-activated cell sorting to create DR-enriched and DR-depleted populations from a single melanoma cell line heterogeneous for DR expression. We found that the proliferation of allogeneic T cells (measured by the 3H-TdR uptake) cultured with the DR-enriched and -depleted melanoma cell populations was directly related to the amount of the HLA-DR antigen expressed. These results indicate that in performance of experiments using melanoma cell lines quantitative assessment of antigenic expression is important, particularly if the function of a specific antigen is under examination. Further, our data clearly identify the HLA-DR antigen on melanoma cells as a participant in allogeneic lymphocyte stimulation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00199902
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  • 3
    Digitale Medien
    Digitale Medien
    Monoclonal antibody detection of a circulating tumor-associated antigen. II. A longitudinal evaluation of patients with colorectal cancer (1982)
    Springer
    Journal of clinical immunology 2 (1982), S. 141-149 
    Details
    ISSN: 1573-2592
    Schlagwort(e): Monoclonal anti-colorectal carcinoma antibodies ; carcinoembryonic antigen ; gastrointestinal cancer
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract An antigen identified by two monoclonal anti-colorectal cancer antibodies was studied in sera of 85 patients who had a resection of their primary colorectal cancer. Preoperative and postoperative serum samples and sera collected every 3 months for at least 1 year were included in this study. The levels of these antigens were compared to the carcinoembryonic antigen (CEA) levels. Sixty-six patients had at least one antigen elevated in the preoperative period. Malignancy recurred in 10 patients. In 8 of these the recurrence could have been predicted by the persistence or rise in antigen levels 3 to 18 months prior to the detection of the recurrence by current clinical methods. The data suggest that the assays for these antigens are valuable prognostic aids for making clinical therapeutic decisions and appropriately stratifying patients for clinical trials.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00916898
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  • 4
    Digitale Medien
    Digitale Medien
    Monoclonal antibody detection of a circulating tumor-associated antigen. I. Presence of antigen in sera of patients with colorectal, gastric, and pancreatic carcinoma (1982)
    Springer
    Journal of clinical immunology 2 (1982), S. 135-140 
    Details
    ISSN: 1573-2592
    Schlagwort(e): Colorectal carcinoma ; gastrointestinal ; radioimmunoassay ; carcinoembryonic antigen ; supplemented serumfree medium ; anti-colorectal carcinoma antibodies
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Hybridoma-secreted monoclonal anti-colorectal carcinoma antibodies 19-9, 52a, and C4 14 bind specifically to cells of colorectal, gastric, and pancreatic carcinoma in tissue culture. The assay for the detection of antigen in human sera is based on the inhibition of binding of monoclonal antibodies to target preparations of colorectal carcinoma cells. Binding of monoclonal antibody 52a was inhibited more than 12% by 163 of 255 sera from patients from various stages of carcinoma of colon and rectum, by 45 of 49 sera from patients with pancreatic carcinoma, and by 8 of 11 sera from patients with gastric carcinoma. By contrast, only 7 of 89 sera from patients with other malignancies and 2 of 108 sera from healthy donors inhibited binding of this monoclonal antibody by more than 12%. These studies show the potential usefulness of monoclonal antibodies in the diagnosis of human malignancy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00916897
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