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  • 1
    Digitale Medien
    Digitale Medien
    Volatile Compounds of Blanched, Fried Blanched, and Baked Blanched Garlic Slices (1994)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 42 (1994), S. 1342-1347 
    Details
    ISSN: 1520-5118
    Quelle: ACS Legacy Archives
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1021/jf00042a018
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  • 2
    Digitale Medien
    Digitale Medien
    Numerical study for influences of material parameters on hydrostatic bulging of metal sheet (1993)
    Springer
    Acta mechanica solida Sinica 6 (1993), S. 131-144 
    Details
    ISSN: 0894-9166
    Schlagwort(e): rigid viscoplastic finite element method ; hydrostatic bulging ; material parameter
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Physik
    Notizen: Abstract In this paper, the influences of various material parameters, the hardening exponent (n), the rate sensitivity (m). the thickness anisotropy parameter (R) and the index M in the Hosford and Hill yield function, on the hydrostatic bulging of a circular clamped sheet of ductile metal materials are analysed by introducting a rigid-viscoplastic finite element method. By numerical studies, an empirical relationship within the average limit thickness strain −ɛ 3 αγ* and the material parameters (n andm) is obtained. Besides, it has been found that the influences of surface shapes of the yield function on the average limit thickness strain can be reflected by the Barlat'sP value which represents the effects ofR andM values.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02207441
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  • 3
    Digitale Medien
    Digitale Medien
    Collateral projections of trigeminal ganglion neurons to both the principal sensory trigeminal and the spinal trigeminal nuclei in the rat (1993)
    Springer
    Experimental brain research 93 (1993), S. 205-212 
    Details
    ISSN: 1432-1106
    Schlagwort(e): Trigeminal ganglion ; Trigeminal nuclei ; Substance P ; Calcitonin gene-related peptide ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Employing a combination of fluorescent retro grade double labelling and immunofluorescence histo chemistry for substance P (SP) and calcitonin gene-relat ed peptide (CGRP), we examined collateral projections from single neurons in the trigeminal ganglion (TG) of the rat to both the principal sensory trigeminal nucleus (Vp) and the oral, interpolar or caudal subnuclei of the spinal trigeminal nucleus (Vo, Vi or Vc). In the rats that were unilaterally injected with fast blue (FB) into the Vp and with diamidino yellow (DY) into the Vo, Vi or Vc, neurons labelled with FB and/or DY were observed in the TG ipsilateral to the injections. Of the labelled TG neurons, about 2% were double labelled with both trac ers in the rats that were injected with FB into the Vp and with DY into the Vo or Vi, and about 10% were double labelled in the rats that were injected with FB into the Vp and with DY into the Vc. The results indicate that TG neurons sending their axons to the Vp project, by way of axon collaterals, to the Vc more frequently than to the Vo or Vi. Some of the TG neurons double labelled with FB and DY exhibited SP-or CGRP-like immunoreactivity (LI): Of the TG neurons that were double labelled with FB injected into the Vp and with DY injected into the Vo, Vi or Vc, about 38%, 49% and 42%, respectively, displayed SP-LI, and about 54%, 58% and 59%, respectively, showed CGRP-LI. Some of the SP-or CGRP-LI TG neurons that were double labelled with FB and DY were assumed to mediate pain signals to both the Vp and the spinal trigeminal nucleus (Vo, Vi and/or Vc) by way of axon collaterals.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228387
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  • 4
    Digitale Medien
    Digitale Medien
    Expression of polyketide biosynthesis and regulatory genes in heterologous streptomycetes (1991)
    Springer
    Journal of industrial microbiology and biotechnology 7 (1991), S. 163-174 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Hybrid antibiotics ; Polyketide biosynthesis ; Anthracyclines ; Actinorhodin ; Streptomycetes ; Interspecies gene cloning
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary There are now several examples showing that hybrid secondary metabolites can be produced as a result of interspecies cloning of antibiotic biosynthesis genes in streptomycetes. This paper reviews examples of hybrid secondary metabolite production, and examines the underlying biochemical and regulatory principles leading to the formation of hybrid anthraquinones by recombinant anthracycline-producing streptomycetes carrying actinorhodin biosynthesis genes. An anthraquinone, aloesaponarin II, was produced by cloning theactI, actIII, actIV, andactVII genes (pANT12) of actinorhodin biosynthesis pathway fromStreptomyces coelicolor in anthracycline producing streptomycetes.Streptomyces galilaeus strains 31 133 and 31 671, aclacinomycin and 2-hydroxyaklavinone producers, respectively, formed aloesaponarin II as their major polyketide product when transformed with pANT12. Subcloning experiments indicated that a 2.8-kbXhoI fragment containing only theactI andactVII loci was necessary for aloesaponarin II biosynthesis byS. galilaeus 31 133. WhenS. galilaeus 31 671 was transformed with theactI, actVII, andactIV genes, however, the recombinant strain produced two novel anthraquinones, desoxyerythrolaccin and 1-0-methyldesoxyerythrolaccin. WhenS. galilaeus 31671 was transformed with only the intactactIII gene (pANT45), aklavinone was formed exclusively. These experiments indicate a function for theactIII gene, which is the reduction of the keto group at C-9 from the carboxyl terminus of the assembled polyketide to the corresponding secondary alcohol. The effects of three regulatory loci,dauG, dnrR1, andasaA, on the production of natural and hybrid polyketides were also shown.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01575879
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  • 5
    Digitale Medien
    Digitale Medien
    Transcriptional organization and regulation of the nosiheptide resistance gene inStreptomyces actuosus (1991)
    Springer
    Journal of industrial microbiology and biotechnology 8 (1991), S. 1-12 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Nosiheptide resistance ; Thiopeptide antibiotics ; Streptomyces ; Antitermination ; Regulation of transcription
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary The nosiheptide resistance gene (nshR) and a putative regulatory gene (nshA) are found together on a 2326 bpBamHI-PstI DNA fragment isolated fromStreptomyces actuosus ATCC 25421. The putative regulatory gene,nshA, situated upstream from the nosiheptide resistance gene in the 2326 bp DNA fragment, contains apparent DNA-binding and RNA-binding domains. Interruption ofnshA in the chromosome ofS. actuosus alters nosiheptide production, suggesting thatnshA is involved in regulation of nosiheptide biosynthesis. Two transcription initiation sites were found upstream ofnshA as demonstrated by high-resolution Sl nuclease mapping. A weak transcription start site fornshR was found which initiated transcription from the first nucleotide of the open reading frame. Although a stem-loop structure with apparent termination activity was found betweennshA andnshR, readthrough of transcription betweennshA andnshR was demonstrated by S1 nuclease mapping of the 3′ terminus of thenshA transcript. Time-course S1 experiments of the three promoters (nshA-pl, nshA-p2, nshR-p) indicated highly regulated differential expression of the promoters.nshA-p2 is a strong, constitutive promoter whereasnshA-pl being regulated temporally with maximal activity at 96 h. Approximately 30% of the totalnshA-p1/p2 transcript reads through the terminator and into thenshR gene, accounting for more than half of the total steady-statenshR transcript. The implications of the regulation ofnshA andnshR gene expression, as well as the expression of two other linked genes, are presented.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01575585
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  • 6
    Digitale Medien
    Digitale Medien
    An INAA study of trace elements in hair samples from two children on different sampling dates (1991)
    Springer
    Journal of radioanalytical and nuclear chemistry 155 (1991), S. 325-333 
    Details
    ISSN: 1588-2780
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Energietechnik
    Notizen: Abstract A study of trace elements in hair samples from two children of different sex on different sampling dates has been carried out using INAA. Using several combinations of irradiation, cooling and counting times, about thirty elements were quantitatively determined. Some conclusions could be drawn from the data obtained.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02163879
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  • 7
    Digitale Medien
    Digitale Medien
    RNAA of trace iridium in Precambrian-Cambrian boundary samples by thiourea type chelate resin separation (1991)
    Springer
    Journal of radioanalytical and nuclear chemistry 151 (1991), S. 107-111 
    Details
    ISSN: 1588-2780
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Energietechnik
    Notizen: Abstract A RNAA procedure is described for the determination of trace Ir in Precambrian-Cambrian boundary samples. After irradiation, the powdered sample is transferred to a graphite crucible to expel the massive silicon with mixed acid (HF−HCl−HNO3) by heating. The residue is then fused with mixed fusion (Na2O2−NaOH) in a muffle furnace at 700°C for 15 minutes. After cooling, the fused mixture is leached with hot water. The final solution is adjusted to pH 1.5–2.0 and then passed through a column filled with thiourea type chelate resin. The resin absorbed with192Ir is measured for 4000–10 000 s by means of SCORPIO-3000 multi-channel computer — Ge(Li) detector system. Experiments with radioactive tracer are carried aout for checking radiochemical separation yield. The accuracy and precision of the method are evaluated by the analysis of U.S. geological SRMs DTS-1 and AG-Bohor-1. The method is used for the determination of trace Ir in several sets of Precambrian-Cambrian boundary samples collected from Yunnan province in China and the Ir anomaly is observed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02040136
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