ISSN:
1573-0778
Schlagwort(e):
Chinese hamster ovary (CHO) cells
;
vitamins
;
ascorbic acid
;
choline
;
nicotinamide
;
thiamine
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Abstract The concentrations of four vitamins, ascorbic acid, nicotinamide, choline and thiamine were evaluated in the culture supernatant of Chinese hamster ovary (CHO) cells. The media used were α-modified Eagle's minimum essential medium (MEM-α) supplemented with 10% fetal calf serum, and a 1:1 mixture of Ham's F12 and Dulbecco's modified Eagle's medium (DME/F12), containing neither serum nor protein. The reference experiment without cells revealed instability of ascorbic acid and thiamine. Moreover, a significant amount of each vitamin decreased in the culture supernatant. The possibility of growth limitation by vitamin depletion is strongly suggested.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00563784
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