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  • 1995-1999  (3)
  • 1960-1964  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Hypotrichomonas acosta (Moskowitz) from Reptiles. 11. Physiology. (1960)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 7 (1960), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Three strains of Hypotrichomonas acosta were isolated in axenic culture. Attempts to develop a defined medium directly from a defined medium suitable for Tetrahymena pyriformis were unsuccessful. Development of partly defined media by substitution for undefined materials in Diamond's medium were more successful. Horse serum was replaced by 1 mg. % TEM-4T (a diacetyl tartaric acid ester of monoglycerides from tallow) and 0.5 mg.% cholesterol. Yeast extract was replaced by a mixture of ribonucleotides. Inclusion of several additional components permitted reducing the Trypticase concentration from 2% to 0.25%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1960.tb05988.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Trichomonads from Poikilotherms: Nutritional and Physiological Notes (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Several trichomonads from poikilotherms (Hypotrichomonas acosta, Monocercomonas colubrorum and M. sp., Trichomonas batrachorum, and Tritrichomonas augusta) grew in a nearly-defined medium to a level of 10–40% that permitted with media containing casein digests; T. augusta (4 strains) grew best. The defined medium contained TEM-4T (a semisynthetic fat based on beef-tallow fatty acids) and ascorbyl “palmitate” as sources of fatty acids. Besides ascorbyl palmitate, thiomalic and ascorbic acids, and tocopherol served as reducers and antiöxidants. High concentrations of nucleotides (adenylic, cytidylic, guanylic, and uridylic acids) seemed necessary. Indigo carmine was a useful redox indicator; the flagellates never grew in the oxidized zone. Extra trace elements and amino acids or rich natural media permitted better growth at about 34.5°C—the upper temperature limit for these trichomonads, raising the issue of a biochemical basis of a thermal barrier to the invasion of warmblooded hosts. Except for the limited carbohydrate in the nucleotides, carbohydrate could be omitted, the main substrates being a combination of glycerol, acetate, and gylcero-phosphate, posing the question of the identity of the anaerobic, presumably non-glycolytic, energy-yielding pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02651.x
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  • 3
    Electronic Resource
    Electronic Resource
    Growth and Physiology of Foraminifera in the Laboratory; Part 4 — Monoxenic Culture of an Allogromiid with Notes on its Morphology (1963)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 10 (1963), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. A polymorphic allogromiid (our strain NF) was isolated in monoxenic culture but attempts at its axenic culture failed. Growth of monoxenic cultures was stimulated by various metals and vitamins. The morphology and life cycle of this allogromiid with a prominent collar have been studied in detail. It varied in form from ovoid to elongate bioral “Shepheardella-like” forms and to irregular polyoral organisms. The organisms ranged in length from 56–385 μ (118 μ± 50.54) and in width from 35–385 μ (99 μ± 39.34). The number of nuclei per organism averaged 8.9 ± 6.3 (range 1–40). Reproduction of “Shepheardella-like” forms was by binary fission. Three types of budding have been observed and, rarely, schizogony. No evidence for sexual reproduction has thus far been seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1963.tb01697.x
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  • 4
    Electronic Resource
    Electronic Resource
    Patterns of spiral tip motion in cardiac tissues (1998)
    Woodbury, NY : American Institute of Physics (AIP)
    Chaos 8 (1998), S. 137-148 
    Details
    ISSN: 1089-7682
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In support of the spiral wave theory of reentry, simulation studies and animal models have been utilized to show various patterns of spiral wave tip motion such as meandering and drifting. However, the demonstration of these or any other patterns in cardiac tissues have been limited. Whether such patterns of spiral tip motion are commonly observed in fibrillating cardiac tissues is unknown, and whether such patterns form the basis of ventricular tachycardia or fibrillation remain debatable. Using a computerized dynamic activation display, 108 episodes of atrial and ventricular tachycardia and fibrillation in isolated and intact canine cardiac tissues, as well as in vitro swine and myopathic human cardiac tissues, were analyzed for patterns of nonstationary, spiral wave tip motion. Among them, 46 episodes were from normal animal myocardium without pharmacological perturbations, 50 samples were from normal animal myocardium, either treated with drugs or had chemical ablation of the subendocardium, and 12 samples were from diseased human hearts. Among the total episodes, 11 of them had obvious nonstationary spiral tip motion with a life span of 〉2 cycles and with consecutive reentrant paths distinct from each other. Four patterns were observed: (1) meandering with an inward petal flower in 2; (2) meandering with outward petals in 5; (3) irregularly concentric in 3 (core moving about a common center); and (4) drift in 1 (linear core movement). The life span of a single nonstationary spiral wave lasted no more than 7 complete cycles with a mean of 4.6±4.3, and a median of 4.5 cycles in our samples. Conclusion: (1) Patently evident nonstationary spiral waves with long life spans were uncommon in our sample of mostly normal cardiac tissues, thus making a single meandering spiral wave an unlikely major mechanism of fibrillation in normal ventricular myocardium. (2) A tendency toward four patterns of nonstationary spiral tip motion was observed. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.166294
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  • 5
    Electronic Resource
    Electronic Resource
    Hypotrichomonas acosta (Moskowitz) Gen. nov. from Reptiles. I. Structure and Division. (1960)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 7 (1960), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Hypotrichomonas gen nov., with a feeble undulating membrane but lacking a costa, is placed in the family Monocercomonadidae Kirby of the order Trichomonadida Kirby. H. acosta (Moskowitz) from squamate reptiles is designated as the type species. It has 3 unequal anterior flagella. The proximal part of its recurrent flagellum is attached to the surface of the body along an accessory filament. A low membrane with typically a few undulations extends between the accessory filament and the body surface. The membrane, which varies greatly in length in relation to the length of the body among strains of the species, is not supported by a costa. The recurrent flagellum continues as a free posterior flagellum beyond the end of the undulating membrane and terminates in an acroneme. The capitulum of the axostyle, spatulate or scoop-shaped, is closely applied to the anteriorly located nucleus. The capitulum extends anteriorly into a crescent-shaped pelta. The relatively stout trunk of the axostyle is straight or twisted. It projects for some distance from the posterior body surface, and the terminal segment of the projecting part tapers gradually to a point. The parabasal apparatus consists of a rod- or shaped body and of a filament or filaments.In division, the full complement of flagella in each of the mastigonts is restored relatively late during division but before cytokinesis. One of the daughter individuals retains the parental undulating membrane; the other develops a new organelle. The development of the new membrane is first marked by the appearance of an acroneme, presumably recurrent flagellum, early in division. The proximal part of this flagellum becomes progressively attached to the body surface. The process of attachment is paralleled by differentiation of the accessory filament and is followed by the appearance of the undulating membrane. The parental axostyle is resorbed during division, and each daughter individual develops this organelle de wove. In the very early stages of division the parabasal consisted of two arms and in all more advanced stages of a single rod-shaped body applied to a single filament.The significance of Hypotrichonionas in the evolution of trichomonads is discussed. Its morphology and morphogenesis suggest that this genus may represent an important step in the evolution of Trichomonadidae from Monocercomonadidae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1960.tb05987.x
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  • 6
    Electronic Resource
    Electronic Resource
    Glenodinium halli n. sp. and Gyrodinium instriatum n. sp., Dinoflagellates from New York Waters (1963)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 10 (1963), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Two new dinoflagellates, isolated from Long Island Sound and maintained axenically, are described. Gyrodinium instriatum n. sp. changes shape and size as cultures age. Glenodinium halli n. sp. is a delicately-armored form whose plate structure differs slightly from the present diagnosis of the genus, but not sufficiently like any other genus in morphology or life history for inclusion elsewhere. In G. halli the size of the motile form and predominance of motile or vegetative stages are nutrient dependent. Both dinoflagellates illustrate the need for morphological descriptions based upon continuous culture methods in defined media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1963.tb01659.x
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  • 7
    Electronic Resource
    Electronic Resource
    TULP1 mutation in two extended Dominican kindreds with autosomal recessive Retinitis pigmentosa (1998)
    [s.l.] : Nature Publishing Group
    Nature genetics 18 (1998), S. 177-179 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The RP14 autosomal recessive Retinitis pigmentosa (arRP) locus has been mapped to a 2cM region of chromosome 6p21.3 (refs 1–3). TULP1 (the gene encoding tubby-like protein 1) is a candidate target for the disease mutation because it maps to the RP14 minimum genetic region and because a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng0298-177
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  • 8
    Electronic Resource
    Electronic Resource
    The product of hedgehog autoproteolytic cleavage active in local and long-range signalling (1995)
    [s.l.] : Nature Publishing Group
    Nature 374 (1995), S. 363-366 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To characterize the signalling molecules produced from the uncleaved Hh precursor U, we determined the site of autoproteolysis and the structures of the cleaved products using a purified Hh protein from a bacterial source. This purified protein, His6C, contains primarily carboxy-terminal sequences ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/374363a0
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