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  • 2000-2004  (10)
  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Macmillian Magazines Ltd.
    Nature 416 (2002), S. 168-171 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The oceanic carbon cycle is mainly determined by the combined activities of bacteria and phytoplankton, but the interdependence of climate, the carbon cycle and the microbes is not well understood. To elucidate this interdependence, we performed high-frequency sampling of sea water along a ...
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Freshwater biology 48 (2003), S. 0 
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. We performed a mesocosm experiment to investigate the structuring and cascading effects of two predominant crustacean mesozooplankton groups on microbial food web components. The natural summer plankton community of a mesotrophic lake was exposed to density gradients of Daphnia and copepods. Regression analysis was used to reveal top–down impacts of mesozooplankton on protists and bacteria after days 9 and 15.2. Selective grazing by copepods caused a clear trophic cascade via ciliates to nanoplankton. Medium-sized (20–40 μm) ciliates (mainly Oligotrichida) were particularly negatively affected by copepods whereas nanociliates (mainly Prostomatida) became more abundant. Phototrophic and heterotrophic nanoflagellates increased significantly with increasing copepod biomass, which we interpret as an indirect response to reduced grazing pressure from the medium-sized ciliates.3. In Daphnia-treatments, ciliates of all size classes as well as nanoflagellates were reduced directly but the overall predation effect became most strongly visible after 15 days at higher Daphnia biomass.4. The response of bacterioplankton involved only modest changes in bacterial biomass and cell-size distribution along the zooplankton gradients. Increasing zooplankton biomass resulted either in a reduction (with Daphnia) or in an increase (with copepods) of bacterial biovolume, activity and production. Patterns of bacterial diversity, as measured by polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE), showed no distinct grouping after 9 days, whereas a clear treatment-coupled similarity clustering occurred after 15 days.5. The experiment demonstrated that zooplankton-mediated predatory interactions cascade down to the bacterial level, but also revealed that changes occurred rather slowly in this summer plankton community and were most pronounced with respect to bacterial activity and composition.
    Type of Medium: Electronic Resource
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  • 3
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    Marcel Decker
    In:  In: Enzymes in the environment. , ed. by Burns, R. and Dick, R. Marcel Decker, New York, USA, pp. 73-107. ISBN 0-8247-0614-5
    Publication Date: 2020-04-01
    Type: Book chapter , NonPeerReviewed
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  • 4
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    Unknown
    Marcel Decker
    In:  In: Enzymes in the environment. , ed. by Burns, R. G. and Dick, R. P. Marcel Decker, New York, Basel, pp. 73-107. ISBN 0-8247-0614-5
    Publication Date: 2020-04-06
    Type: Book chapter , NonPeerReviewed
    Format: text
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  • 5
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    Unknown
    Springer
    In:  Hydrobiologia, 493 . pp. 187-200.
    Publication Date: 2018-07-02
    Description: Phosphatase is a key-enzyme in the marine environment, although life in the sea is normally not P limited. Expression of phosphatase in algae is generally regulated by the prevailing external concentration of inorganic phosphate, but the internal N:P ratio may also play a role. For bacteria, additional mechanisms like their C and N demands may be important. This is suggested by high phosphatase activities occasionally measured in eutrophic or deep water in the presence of relatively high phosphate concentrations. The distribution of phosphatase activity among the particulate and the dissolved fractions is highly variable. In particular, the dissolved fraction can contribute considerably to the total phosphatase activity (up to 70%), which differs from the pattern of other hydrolytic ectoenzymes. Parts of this fraction may originate from marine protozoa. The contribution of bacteria and phytoplankton to the particle-associated fraction of phosphatase is extremely variable, depending on P-availability, the dominant organisms, water depth and environmental factors. Community analysis revealed that bacteria attached to marine snow and N2-fixing cyanobacteria were frequently strong producers of phosphatase. Field studies carried out on a great variety of marine regions suggest that phosphatase activity is generally a good indicator of the P status of phytoplankton. Several heat-stable or heat-labile phosphatases, isolated from marine organisms living in extreme or other environments have been recommended for biotechnological applications.
    Type: Article , PeerReviewed
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  • 6
    Publication Date: 2017-01-16
    Description: 1. We performed a mesocosm experiment to investigate the structuring and cascading effects of two predominant crustacean mesozooplankton groups on microbial food web components. The natural summer plankton community of a mesotrophic lake was exposed to density gradients of Daphnia and copepods. Regression analysis was used to reveal top-down impacts of mesozooplankton on protists and bacteria after days 9 and 15. 2. Selective grazing by copepods caused a clear trophic cascade via ciliates to nanoplankton. Medium-sized (20-40 mum) ciliates (mainly Oligotrichida) were particularly negatively affected by copepods whereas nanociliates (mainly Prostomatida) became more abundant. Phototrophic and heterotrophic nanoflagellates increased significantly with increasing copepod biomass, which we interpret as an indirect response to reduced grazing pressure from the medium-sized ciliates. 3. In Daphnia-treatments, ciliates of all size classes as well as nanoflagellates were reduced directly but the overall predation effect became most strongly visible after 15 days at higher Daphnia biomass. 4. The response of bacterioplankton involved only modest changes in bacterial biomass and cell-size distribution along the zooplankton gradients. Increasing zooplankton biomass resulted either in a reduction (with Daphnia) or in an increase (with copepods) of bacterial biovolume, activity and production. Patterns of bacterial diversity, as measured by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), showed no distinct grouping after 9 days, whereas a clear treatment-coupled similarity clustering occurred after 15 days. 5. The experiment demonstrated that zooplankton-mediated predatory interactions cascade down to the bacterial level, but also revealed that changes occurred rather slowly in this summer plankton community and were most pronounced with respect to bacterial activity and composition.
    Type: Article , PeerReviewed
    Format: text
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  • 7
    Publication Date: 2017-02-28
    Description: The oceanic carbon cycle is mainly determined by the combined activities of bacteria and phytoplankton, but the interdependence of climate, the carbon cycle and the microbes is not well understood. To elucidate this interdependence, we performed high-frequency sampling of sea water along a north-south transect of the Atlantic Ocean. Here we report that the interaction of bacteria and phytoplankton is closely related to the meridional profile of water temperature, a variable directly dependent on climate. Water temperature was positively correlated with the ratio of bacterial production to primary production, and, more strongly, with the ratio of bacterial carbon demand to primary production. In warm latitudes (25 degrees N to 30 degrees S), we observed alternating patches of predominantly heterotrophic and autotrophic community metabolism. The calculated regression lines (for data north and south of the Equator) between temperature and the ratio of bacterial production to primary production give a maximum value for this ratio of 40% in the oligotrophic equatorial regions. Taking into account a bacterial growth efficiency of 30%, the resulting area of net heterotrophy (where the bacterial carbon demand for growth plus respiration exceeds phytoplankton carbon fixation) expands from 8 degrees N (27 degrees C) to 20 degrees S (23 degrees C). This suggests an output of CO2 from parts of the ocean to the atmosphere.
    Type: Article , PeerReviewed
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  • 8
    Publication Date: 2018-04-26
    Description: Agar oligosaccharides in the neoagarobiose series were prepared by partial enzyme hydrolysis, separated on Biogel P2 and P4, and analyzed by high-performance anion exchange chromatography with pulsed amperometric detection, yielding neoagarosaccharide fractions with a disaccharide repetition degree ranging from 1 (neoagarobiose) to more than 8 (neoagarohexadecaose). These fractions were analyzed for their biological activity toward the marine red alga Gracilaria conferta (Schousboe ex Montagne) J. et G. Feldmann in terms of increase of oxygen consumption, release of hydrogen peroxide, elimination of epiphytic bacteria, and induction of thallus tip bleaching. The structure–activity and dose–response relationships of neoagarosaccharides were very similar in the respiratory and oxidative burst responses and in their bactericidal properties, with neoagarosaccharides consisting of 6 to 8 disaccharide repeating units being the most active. All these responses were competitively inhibited by the reduced form of neoagarohexaose, neoagarohexaitol. In contrast, the tip-bleaching response was light dependent, required much higher concentrations of neoagarosaccharides, and was not inhibited by neoagarohexaitol, suggesting that it is an unspecific oxidative stress reaction. Putative structural effects on the recognition of endogenous agar-oligosaccharide elicitors by G. conferta are discussed.
    Type: Article , PeerReviewed
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  • 9
    Publication Date: 2018-06-01
    Description: The seasonal development of bacteria was studied in the hypertrophic coastal lagoon Ciénaga Grande de Santa Marta (Caribbean coast of Colombia). This large but only 1.5 m deep lagoon is subject to strong seasonal variations of salinity from almost fully marine (April/May) to brackish conditions in October/November. Chlorophyll ranged from 6 to 182 μg L−1, and gross primary production amounted to 1690 g C m−2 per year. Total bacterial number (TBN) ranged from 6.5 to 90.5 × 109 cells L−1 and bacterial biomass (BBM) from 77 to 1542 μg C L−1, which are among the highest ever reported for natural coastal waters. Neither TBN nor BBM varied significantly with salinity, phytoplankton or seston concentrations. Only the bacterial mean cell volume showed a significant relation to salinity, being highest (0.066 μm3) during the period of increasing and lowest (0.032 μm3) during decreasing salinity. Bacterial protein accounted for 24% (19–26%) and phytoplankton protein for 57% (53–71%) of total seston protein. The ratio (annual mean) of bacterial carbon to phytoplankton carbon was 0.44 (range 0.04–1.43). At low phytoplankton abundance [chlorophyll a (Chl a) 〈 25 μg L−1], bacterial carbon was almost equal to phytoplankton biomass (i.e. the mean ratio was 1.04). In contrast, at Chl a 〉 100 μg L−1, BBM was low compared to phytoplankton biomass (the mean ratio was 0.16). In general, BBM varied less than phytoplankton biomass. Most probably, the missing correlation between bacterial and phytoplankton variables was due to (i) organic material partly derived from allochthonous sources serving as food resource for bacteria and (ii) a strong resuspension of bacteria from the sediment caused by frequent wind-induced mixing of the very shallow lagoon.
    Type: Article , PeerReviewed
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  • 10
    Publication Date: 2018-05-29
    Description: Water and sediment quality conditions in and around a cage culture unit located in Wismar Bay (Western Baltic Sea, Germany) were investigated during summer 1994 using bacterial group indicators (Enterobacteriaceae, saprophytic bacteria and Vibrio, determined on selective media). Fish were fed once daily with extruded commercial pellets. Over a period of 8 days samples were taken before and after feeding at different sampling points close to or at the cages. The results showed that a drastic (up to 10 times) increase in different bacterial groups occurred inside the cages 10 min after feeding as well as occasionally in the surrounding water. Additional electron-microscopic observations of the bacterial biofilm on the cage nets revealed that a decrease (in comparison with the original number) in total bacteria on the net materials of 9.5 up to 42.8% occurred after feeding. It was concluded that water mixing caused by frenzied swimming just before and during feeding caused a release of bacteria which were growing vigorously on the surfaces of the cage system. Since the pathogenity and hydrophobicity of bacteria are closely related, this process of redistribution of bacteria into the water column could constitute an important stress factor for fish, which could probably also have a negative effect on fish production.
    Type: Article , PeerReviewed
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