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  • 1
    Online Resource
    Online Resource
    Presença do papilomavirus humano em lesões malignas de mucosa oral
    FapUNIFESP (SciELO) ; 2002
    In:  Revista da Sociedade Brasileira de Medicina Tropical Vol. 35, No. 5 ( 2002-10), p. 439-444
    Details
    In: Revista da Sociedade Brasileira de Medicina Tropical, FapUNIFESP (SciELO), Vol. 35, No. 5 ( 2002-10), p. 439-444
    Abstract: The purpose of this study was to investigate the prevalence of human papillomavirus 6/11 and 16/18 in patients, with oral lesions clinically diagnosed as leucoplakia, attending the School of Dentistry, University of São Paulo State/UNESP, Brazil. After paraffin embedded process, in the sections staining with H & E, 30 biopsies were screened and separated on 3 groups: 10 oral lesions without dysplasia, 10 with dysplasia, and 10 with invasive squamous cell carcinoma. The lesions with dysplasia were classified in agreement with Van Der Wall's histopathological standard method. Oral lesions were investigated for the presence of human papillomavirus (HPV) by in situ hybridization with wide-spectrum, 6/11 and 16/18 biotinylated probes. HPV 16/18 was found in 20% (n=2) of the leucoplakia with severe-degree dysplasia. The presence of HPV 16/18 in malignant lesions suggests its importance as a risk factor for oral carcinogenesis.
    Type of Medium: Online Resource
    ISSN: 0037-8682
    URL: Article
    DOI: 10.1590/S0037-86822002000500003
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2002
    detail.hit.zdb_id: 2028921-2
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  • 2
    Online Resource
    Online Resource
    Freqüência de híbridos em cruzamentos controlados de citros: cultivo de sementes versus cultivo in vitro de embriões
    FapUNIFESP (SciELO) ; 2002
    In:  Pesquisa Agropecuária Brasileira Vol. 37, No. 7 ( 2002-07), p. 981-988
    Details
    In: Pesquisa Agropecuária Brasileira, FapUNIFESP (SciELO), Vol. 37, No. 7 ( 2002-07), p. 981-988
    Abstract: The frequency of citrus hybrids obtained from controlled crossings was studied, comparing seed germination in seedbeds with embryos cultivated in vitro. The effect of peeling off the seed teguments on the germination was also studied. The 'Clementina Palazelli' and 'Sunki' mandarins, 'Rangpur Santa Bárbara' lime, 'Volkamer Catania 2' lemon and sour oranges 'Common' and 'Double Calice' were used as female parents. The male parents were Poncirus trifoliata and hybrids of this species. A completely randomized design was used, with three seed treatments: intact; without the outer tegument (testa); and, without both the outer and inner (tegmen) teguments. The in vitro embryo culture was used as control. Evaluations included the speed of seed germination, percentage of germination and polyembryony, size and number of embryos per seed and percentage of hybrid seedlings. The removal of seed teguments accelerated the germination in all varieties; improved the percentage of germination only in 'Sunki' mandarin and 'Volkamer' lemon. The percentage of zygotic seedlings that were rescued was higher in the in vitro embryo culture, in such a way that the advantage in using this technique was directly proportional to the degree of polyembryony of the female parents used in the crosses.
    Type of Medium: Online Resource
    ISSN: 0100-204X
    URL: Article
    DOI: 10.1590/S0100-204X2002000700012
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2002
    detail.hit.zdb_id: 2053197-7
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