In:
FEBS Letters, Wiley, Vol. 551, No. 1-3 ( 2003-09-11), p. 20-24
Kurzfassung:
In whole‐cell recordings on single HeLa cells, the hypertonic activation of a cation conductance with a selectivity ratio P Na :P Li :P K :P Cs :P NMDG :P Ca :P Cl of 1.00:0.86:0.84:0.56:0.10:0.07:0.15 was observed. This (non‐selective) cation conductance was reduced to 59 and 30% of maximal stimulation by Gd 3+ and flufenamate, respectively, but it was insensitive to amiloride (with each compound applied at 100 μmol/l). As was determined by the Coulter counter technique, the cation conductance was the main mechanism of regulatory volume increase (RVI) in HeLa cells. Whereas a significant contribution of Na + /H + antiport was also detectable, Na + ‐K + ‐2Cl − symport most likely did not contribute to RVI.
Materialart:
Online-Ressource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(03)00868-8
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2003
ZDB Id:
1460391-3
SSG:
12
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