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  • 1
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2009. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Bulletin of the American Meteorological Society 90 (2009):459-480, doi:10.1175/2008BAMS2608.1.
    Description: The Indian Ocean is unique among the three tropical ocean basins in that it is blocked at 25°N by the Asian landmass. Seasonal heating and cooling of the land sets the stage for dramatic monsoon wind reversals, strong ocean–atmosphere interactions, and intense seasonal rains over the Indian subcontinent, Southeast Asia, East Africa, and Australia. Recurrence of these monsoon rains is critical to agricultural production that supports a third of the world's population. The Indian Ocean also remotely influences the evolution of El Niño–Southern Oscillation (ENSO), the North Atlantic Oscillation (NAO), North American weather, and hurricane activity. Despite its importance in the regional and global climate system though, the Indian Ocean is the most poorly observed and least well understood of the three tropical oceans. This article describes the Research Moored Array for African–Asian–Australian Monsoon Analysis and Prediction (RAMA), a new observational network designed to address outstanding scientific questions related to Indian Ocean variability and the monsoons. RAMA is a multinationally supported element of the Indian Ocean Observing System (IndOOS), a combination of complementary satellite and in situ measurement platforms for climate research and forecasting. The article discusses the scientific rationale, design criteria, and implementation of the array. Initial RAMA data are presented to illustrate how they contribute to improved documentation and understanding of phenomena in the region. Applications of the data for societal benefit are also described.
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
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  • 2
    Publication Date: 2022-05-26
    Description: Author Posting. © American Meteorological Society, 2009. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Bulletin of the American Meteorological Society 90 (2009): ES5-ES8, doi:10.1175/2008BAMS2608.2.
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
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  • 3
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    OceanObs'09
    In:  In: Proceedings of the "OceanObs'09: Sustained Ocean Observations and Information for Society". , ed. by Hall, J., Harrison, D. E. and Stammer, D. ESA Publication, WPP-306 . OceanObs'09, Venice, Italy.
    Publication Date: 2012-07-06
    Type: Book chapter , NonPeerReviewed
    Format: text
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  • 4
    Publication Date: 2012-08-01
    Description: Sorting of membrane proteins into intracellular organelles is crucial for cell function. Viruses exploit intracellular transport and retention systems to concentrate envelope proteins at the site of virus budding. In pestiviruses, a group of important pathogens of pigs and ruminants closely related to human hepatitis C virus, the E rns protein translated from the viral RNA is secreted from the infected cells and found in the serum of infected animals. Secretion of the protein is regarded as crucial for its function as a viral virulence factor associated with its RNase activity. However, ~95% of the E rns molecules are retained within the infected cell. Fusion of different E rns fragments to the C terminus of CD72 allowed identification of a retention signal within the C-terminal 65 aa of the viral protein. This C-terminal sequence represents its membrane anchor and folds into an amphipathic helix binding in-plane to the membrane surface. Residues L183, I190, and L208 are important for intracellular location of E rns . Presentation of the retention signal on the cytoplasmic instead of the luminal face of the ER membrane in CD8α fusion proteins still led to retention. Thus, E rns contains in its C-terminal amphipathic helix an intracellular retention signal that is active on both faces of the membrane.—Burrack, S., Aberle, D., Bürck, J., Ulrich, A. S., Meyers, G. A new type of intracellular retention signal identified in a pestivirus structural glycoprotein.
    Print ISSN: 0892-6638
    Electronic ISSN: 1530-6860
    Topics: Biology
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  • 5
    Publication Date: 2011-07-13
    Description: Impaired immune functions leading to primary immunodeficiencies often correlate with paradoxical autoimmune complications; patients with hyper-IgM syndromes who are deficient in activation-induced cytidine deaminase (AID), which is required for class-switch recombination and somatic hypermutation, are prone to develop autoimmune diseases. To investigate the impact of AID-deficiency on early B-cell tolerance checkpoints in humans, we tested by ELISA the reactivity of recombinant antibodies isolated from single B cells from AID-deficient patients. New emigrant/transitional and mature naive B cells from AID-deficient patients express an abnormal Ig repertoire and high frequencies of autoreactive antibodies, demonstrating that AID is required for the establishment of both central and peripheral B-cell tolerance. In addition, B-cell tolerance was further breached in AID-deficient patients as illustrated by the detection of anti-nuclear IgM antibodies in the serum of all patients. Thus, we identified a major and previously unsuspected role for AID in the removal of developing autoreactive B cells in humans.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 6
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    The American Society for Biochemistry and Molecular Biology (ASBMB)
    Publication Date: 2014-04-26
    Description: The calicivirus minor capsid protein VP2 is expressed via termination/reinitiation. This process depends on an upstream sequence element denoted termination upstream ribosomal binding site (TURBS). We have shown for feline calicivirus and rabbit hemorrhagic disease virus that the TURBS contains three sequence motifs essential for reinitiation. Motif 1 is conserved among caliciviruses and is complementary to a sequence in the 18 S rRNA leading to the model that hybridization between motif 1 and 18 S rRNA tethers the post-termination ribosome to the mRNA. Motif 2 and motif 2* are proposed to establish a secondary structure positioning the ribosome relative to the start site of the terminal ORF. Here, we analyzed human norovirus (huNV) sequences for the presence and importance of these motifs. The three motifs were identified by sequence analyses in the region upstream of the VP2 start site, and we showed that these motifs are essential for reinitiation of huNV VP2 translation. More detailed analyses revealed that the site of reinitiation is not fixed to a single codon and does not need to be an AUG, even though this codon is clearly preferred. Interestingly, we were able to show that reinitiation can occur at AUG codons downstream of the canonical start/stop site in huNV and feline calicivirus but not in rabbit hemorrhagic disease virus. Although reinitiation at the original start site is independent of the Kozak context, downstream initiation exhibits requirements for start site sequence context known for linear scanning. These analyses on start codon recognition give a more detailed insight into this fascinating mechanism of gene expression.
    Print ISSN: 0021-9258
    Electronic ISSN: 1083-351X
    Topics: Biology , Chemistry and Pharmacology
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