In:
Journal of Phycology, Wiley, Vol. 48, No. 5 ( 2012-10), p. 1232-1247
Abstract:
Field sampling was undertaken to investigate the occurrence of P seudo‐nitzschia P eragallo species in eight locations along the coast of M alaysian B orneo. A total of 108 strains of P seudo‐nitzschia species were isolated, and their morphology examined with SEM and TEM . Additionally, molecular data from nuclear‐encoded partial LSU r DNA , and ITS regions, were characterized. A total of five species were confidently identified based on a combination of distinct morphological characteristics and supporting molecular evidence: P . brasiliana L undholm, H asle & F ryxell, P . cuspidata ( H asle) H asle, P . dolorosa L undholm & M oestrup, P . micropora P riisholm, M oestrup & L undholm, and P . pungens ( G runow) H asle var. pungens . However, one morphotype from S arawak, while somewhat similar to P . caciantha , showed significant morphological distinction from this and any other of the currently described species. Most notably this morphotype possessed a characteristic pore arrangement in the poroids, with the fine pores in each perforation sector arranged in circles. Pair‐wise sequence comparison of the LSU r DNA between this unidentified morphotype and P . caciantha L undholm, M oestrup & H asle, revealed 2.7% genetic divergence. Phylogenetic analyses strongly supported the monophyly of the morphotype. Based upon these supporting data it is here described as a new species, P seudo‐nitzschia circumpora sp. nov. A key to the six species of P seudo‐nitzschia from M alaysian B orneo is presented. Molecular signatures for all species were established based on structural comparisons of ITS 2 r RNA transcripts.
Type of Medium:
Online Resource
ISSN:
0022-3646
,
1529-8817
DOI:
10.1111/jpy.2012.48.issue-5
DOI:
10.1111/j.1529-8817.2012.01213.x
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
281226-5
detail.hit.zdb_id:
1478748-9
SSG:
12
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