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  • 2020-2024  (26)
Publikationsart
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  • 1
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    Effects of paternal investment on sexual immune dimorphism in three species of sex-role reversed pipefish: candidate gene expression (Fluidigm) of females and males in different gravity stages (2020)
    PANGAEA
    Details
    Publikationsdatum: 2023-01-13
    Beschreibung: We sampled the three pipefish species, Nerophis ophidion, Syngnathus rostellatus and Syngnathus typhle in April and May 2016 by snorkelling in seagrass meadows around the Kiel Fjord (54°44'N; 9°53'E). All animals were kept for acclimatisation to laboratory conditions for two weeks in the aquaria at GEOMAR. Males were randomly assigned to one of the three reproductive stage: non-pregnant males, pregnant males and males after parturition. Only males assigned to be sampled at pregnant and after parturition stage were permitted to mate with the females. At mid pregnancy, males were either left in the tanks to be sampled after parturition or sampled immediately. Fish were killed by an overdose of MS222 and their weight and length were measured. Gills were collected and stored in RNAlater (Qiagen) for candidate gene expression analysis. RNA was extracted from gill tissue with RNeasy 96 Universal Tissue Kit (Qiagen) following the manufacturers protocol for animal tissues. RNA yield was measured by spectrometry (NanoDrop ND-1000; peQLab) and 200 ng/µl were used for reverse transcription with QuantiTect®Reverse-Transcription Kit (Qiagen). We used primer pairs for immune system related and metabolism related genes (Beemelmanns and Roth 2016 doi:10.1002/ece3.2391). The expression patterns of genes were measured using a Fluidigm-BioMarkTM system based on 96.96 dynamic arrays (GE-Chip).
    Schlagwort(e): Condition factor; Gravity stage; High Throughput PCR, Fluidigm BioMark HD; Identification; Length, total; Mass; Minus Δ threshold cycle, quantitative polymerase chain reaction; Parental investment; pipefish; resource-allocation trade-off; Sex; sex-role reversal; sexual immune dimorphism; Species
    Materialart: Dataset
    Format: text/tab-separated-values, 3299 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 2
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    Experiment on pipefish Syngnathus typhle: numbers of offspring from pipefish fathers and juvenile size 10 days after hatching (2021)
    PANGAEA
    Details
    Publikationsdatum: 2023-11-02
    Beschreibung: After pipefish males gave birth, juveniles were either placed in tanks containing water with a salinity level of 15 PSU or 7 PSU. This dataset contains the average size of males from the same treatment group, the number of juveniles from each father placed in the respective tank and the percentage of juveniles surviving the first ten days of their life.
    Schlagwort(e): Baltic Sea; Climate change; Experiment; genetic adaptation; Local adaptation; Location; Number of offspring; Origin; phenotypic plasticity; Salinity; Species; Survival; syngnathids; Syngnathus typhle, juvenile; Syngnathus typhle, length; Tank number; Transgenerational plasticity
    Materialart: Dataset
    Format: text/tab-separated-values, 2495 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 3
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    Experiment on pipefish Syngnathus typhle: size and mass of males and females (2021)
    PANGAEA
    Details
    Publikationsdatum: 2023-11-02
    Beschreibung: This dataset contains the total length and weight of all adult pipefish used in this study. Size of pipefish females were measured within a week after they transferred the eggs to the males. Males were measured within a week after giving birth.
    Schlagwort(e): Baltic Sea; Climate change; Experiment; genetic adaptation; Identification; Local adaptation; Location; Origin; phenotypic plasticity; Salinity; Sex; Species; syngnathids; Syngnathus typhle, length; Syngnathus typhle, mass; Tank number; Transgenerational plasticity
    Materialart: Dataset
    Format: text/tab-separated-values, 2988 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 4
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    Gene expression data of pipefish females Syngnathus typhle (minus delta CT) (2021)
    PANGAEA
    Details
    Publikationsdatum: 2023-11-02
    Beschreibung: To obtain signatures of local adaptation and phenotypic plasticity in pipefish originating from different salinity levels we measured targeted gene expression levels. This dataset contains -deltaCT values obtained from real time PCR on a fluidigm chip for all female pipefish and targeted genes used in this experiment.
    Schlagwort(e): Baltic Sea; Climate change; Condition factor; Experiment; genetic adaptation; High Throughput PCR, Fluidigm BioMark HD; Identification; Local adaptation; Location; Minus Δ threshold cycle, quantitative polymerase chain reaction; Origin; phenotypic plasticity; Salinity; Sex; Species; syngnathids; Syngnathus typhle, length; Syngnathus typhle, mass; Tank number; Transgenerational plasticity
    Materialart: Dataset
    Format: text/tab-separated-values, 6820 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 5
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    Gene expression data of pipefish juveniles Syngnathus typhle (minus delta CT) (2021)
    PANGAEA
    Details
    Publikationsdatum: 2023-11-02
    Beschreibung: To obtain signatures of local adaptation, transgenerational plasticity and phenotypic plasticity in pipefish originating from different salinity levels we measured targeted gene expression levels in the the offspring generation. This dataset contains -deltaCT values obtained from real time PCR on a fluidigm chip for all juvenile pipefish and targeted genes used in this experiment.
    Schlagwort(e): Baltic Sea; Climate change; Experiment; genetic adaptation; High Throughput PCR, Fluidigm BioMark HD; Identification; Local adaptation; Location; Minus Δ threshold cycle, quantitative polymerase chain reaction; Origin; phenotypic plasticity; Salinity; Sample code/label; Species; syngnathids; Tank number; Transgenerational plasticity; Treatment
    Materialart: Dataset
    Format: text/tab-separated-values, 34099 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 6
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    Experiment on pipefish Syngnathus typhle: juvenile mortality- infection experiment (2021)
    PANGAEA
    Details
    Publikationsdatum: 2024-01-26
    Beschreibung: Ten days post-hatch, juveniles received one of the four following infection treatments: (i) no injection – control (c), (ii) sham injection of autoclaved seawater (sw) with the equivalent salinity, i.e., 15 or 7 PSU, (iii) injection of Vibrio alginolyticus strain K01M1, which evolved for 90 days under laboratory condition at 15 PSU (v15) or (iv) Vibrio alginolyticus strain K01M1 that had evolved for 90 days under laboratory condition at 7 PSU (v7). One day after the injection, one juvenile was removed and used for the measurment of targeted gene expression. The size of this individual is listed in the dataset as well as the number of juvenile pipefish alive or dead (cummulative) for each treatment, replicate and timepoint of the survival experiment.
    Schlagwort(e): Baltic Sea; Climate change; Day of experiment; Experiment; Fish, alive; Fish, dead; genetic adaptation; Identification; Local adaptation; Location; Origin; phenotypic plasticity; Salinity; Sampling; Species; Survival; syngnathids; Syngnathus typhle, juvenile; Syngnathus typhle, length; Tank number; Transgenerational plasticity; Treatment; Treatment: time after
    Materialart: Dataset
    Format: text/tab-separated-values, 142793 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 7
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    Microsatellite data of pipefish from six sampling locations in the southern Baltic Sea (2021)
    PANGAEA
    Details
    Publikationsdatum: 2024-04-20
    Beschreibung: In this experiment Syngnathus typhle individuals were collected at six different locations along the German Baltic Sea coastline. To analyse the populations structure of the pipefish we isolated genomic DNA from fin clips of collected pipefish female. All 144 female S. typhle samples were genotyped for 11 microsatellite loci, with a minimum of 20 individuals per sampling site. The allele report contains the microsatelite length of the three primer pools used for multiplex PCR. The report gives information, if indivuduals are homozygous (same allele length) or heterozygous (different allele length) for targeted alleles. Some samples were negelected due to too many missing alleles. Allele reports were uploaded in Microchecker and Genetix for analyses of the population structure.
    Schlagwort(e): Baltic Sea; Climate change; FalckensteinerStrand_S.typhle; Fehmarn_S.typhle; Fehmarn, Orth Bay; Flensburg_S.typhle; Flensburg Fjord; genetic adaptation; Hand net; HN; Kiel Fjord, Falckensteiner Strand; Local adaptation; phenotypic plasticity; RuegenNorth_S.typhle; RuegenSouth_S.typhle; Salinity; Salzhaff_S.typhle; Salzhaff, Werder (Salz); Strelasund Grabow (RuegS); syngnathids; The Little Belt; Transgenerational plasticity; Wieker Bodden (RuegN)
    Materialart: Dataset
    Format: application/zip, 46.9 kBytes
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 8
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    Experiment on pipefish Syngnathus typhle to inhabit low salinity areas of the Baltic Sea (2021)
    PANGAEA
    Details
    Publikationsdatum: 2024-04-20
    Beschreibung: The aim of the study was to investigate the mechanisms allowing pipefish to inhabit low salinity areas of the Baltic Sea. The parental Syngnathus typhle generation for this experiment was caught in seagrass meadows of six sampling sites along the German coastline of the Baltic Sea in spring 2017, i.e. in the Flensburg Fjörd, Falckensteiner Strand, Orth Bay next to Fehmarn, Salzhaff and 2 sample sites around Rügen. Three sampling sites are characterized by relatively high salinity conditions (14 - 17 PSU; high salinity origin; H) and three sampling sites by relatively low salinity conditions (7 - 11 PSU; low salinity origin, L; Table 1). Salzhaff was assigned the category low because salinity drops are common after rainfall accompanied with freshwater discharge due to enclosed morphology of the inlet. Therefore, pipefish in Salzhaff are likely to be exposed to salinity levels below 10 PSU. A minimum of 30 non-pregnant males and 30 females were caught snorkelling with hand nets at each sampling site at depths ranging between 0.5 and 2.5 m. At each sampling site, water temperature and salinity were measured from water collected about 1 m below the surface using a salinometer (WTW Cond 330i). The common garden experiment was conducted at the facilities of the GEOMAR (west shore) in spring 2020.
    Schlagwort(e): Baltic Sea; Climate change; genetic adaptation; Local adaptation; phenotypic plasticity; Salinity; syngnathids; Transgenerational plasticity
    Materialart: Dataset
    Format: application/zip, 6 datasets
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 9
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    Evolution of male pregnancy associated with remodeling of canonical vertebrate immunity in seahorses and pipefishes (2020)
    National Academy of Sciences
    Details
    Publikationsdatum: 2023-02-08
    Beschreibung: A fundamental problem for the evolution of pregnancy, the most specialized form of parental investment among vertebrates, is the rejection of the nonself-embryo. Mammals achieve immunological tolerance by down-regulating both major histocompatibility complex pathways (MHC I and II). Although pregnancy has evolved multiple times independently among vertebrates, knowledge of associated immune system adjustments is restricted to mammals. All of them (except monotremata) display full internal pregnancy, making evolutionary reconstructions within the class mammalia meaningless. Here, we study the seahorse and pipefish family (syngnathids) that have evolved male pregnancy across a gradient from external oviparity to internal gestation. We assess how immunological tolerance is achieved by reconstruction of the immune gene repertoire in a comprehensive sample of 12 seahorse and pipefish genomes along the “male pregnancy” gradient together with expression patterns of key immune and pregnancy genes in reproductive tissues. We found that the evolution of pregnancy coincided with a modification of the adaptive immune system. Divergent genomic rearrangements of the MHC II pathway among fully pregnant species were identified in both genera of the syngnathids: The pipefishes (Syngnathus) displayed loss of several genes of the MHC II pathway while seahorses (Hippocampus) featured a highly divergent invariant chain (CD74). Our findings suggest that a trade-off between immunological tolerance and embryo rejection accompanied the evolution of unique male pregnancy. That pipefishes survive in an ocean of microbes without one arm of the adaptive immune defense suggests a high degree of immunological flexibility among vertebrates, which may advance our understanding of immune-deficiency diseases.
    Materialart: Article , PeerReviewed , info:eu-repo/semantics/article
    Format: text
    Format: text
    Format: text
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 10
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    Parental investment and immune dynamics in sex-role reversed pipefishes (2020)
    Public Library of Science
    Details
    Publikationsdatum: 2023-02-08
    Beschreibung: Parental care elevates reproductive success by allocating resources into the upbringing of the offspring. However, it also imposes strong costs for the care-giving parent and can foster sexual dimorphism. Trade-offs between the reproductive system and the immune system may result in differential immunological capacities between the care-providing and the non-care-providing parent. Usually, providing care is restricted to the female sex making it impossible to study a sex-independent influence of parental investment on sexual immune dimorphism. The decoupling of sex-dependent parental investment and their influences on the parental immunological capacity, however, is possible in syngnathids, which evolved the unique male pregnancy on a gradient ranging from a simple carrying of eggs on the trunk (Nerophinae, low paternal investment) to full internal pregnancy (Syngnathus, high paternal investment). In this study, we compared candidate gene expression between females and males of different gravity stages in three species of syngnathids (Syngnathus typhle, Syngnathus rostellatus and Nerophis ophidion) with different male pregnancy intensities to determine how parental investment influences sexual immune dimorphism. While our data failed to detect sexual immune dimorphism in the subset of candidate genes assessed, we show a parental care specific resource-allocation trade-off between investment into pregnancy and immune defense when parental care is provided.
    Materialart: Article , PeerReviewed , info:eu-repo/semantics/article
    Format: text
    Standort Signatur Einschränkungen Verfügbarkeit
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