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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 84 (1980), S. 3072-3075 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Analytical chemistry 53 (1981), S. 1755-1759 
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 11 (1980), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Hybrid cells have been recovered from selective culture medium after fusion of concanavalin-A-activated human lymphocytes with an AKR mouse thymoma (BW 5147). After 6 months of culture twenty-seven out of forty-nine clones still contained human chromosomes. Human chromosome 6 was present in 89% of these clones, and human X in 70%. Clones from one hybrid line contained several human chromosomes. In twelve of the clones carrying human chromosomes, the rosetting with sheep Erythrocytes (SRBC) was 3 times as high as In the BW 5147 cell line. All these clones Carried the human chromosome 6, and eight clones contained the human X chromosome as well. In some of these clones (25%) chromosome 6 was the only human one present. In the two clones In which human chromosome 6 was completely missing, the resetting with SRBC was at the level of the BW line. We therefore suggest that genes on human chromosome 6 are responsible for rosetting with SRBC.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 20 (1984), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: BALB/c mice were injected with an affinity-chromatography-purified placental transferrin receptor preparation. Spleen cells were fused with NS-1 myeloma cells. Sixteen hybrids producing monoclonal antibodies specific for the transferrin receptor and two hybrids specific for transferrin were identified by radioimmunoassay (RIA). Five hybrids were selected for cloning on the basis of antibody specificity and affinity. None of the antibodies inhibited the binding of transferrin to K562 cells. The binding of antibody ID9 to K562 cells was partially inhibited by transferrin or a polyclonal goat anti-transferrin receptor antiserum. Of the five antibodies, two (IIB6 and IIB2) reacted only with the purified receptor and solubilized cells and not with whole cells. The other three antibodies, when tested with normal human cells and leukaemia and tumour cell lines, showed identical reaction patterns. The antibodies precipitated a glycoprotein from K562 cells with an apparent molecular weight of 94,000, estimated from sodium dodecyl sulphate-polyacrylamide gel electrophoretograms run under reducing conditions, and a molecular weight of 188,000 when run under unreduced conditions. All antibodies have a high affinity with Ka values ranging from 1.44 ± 109 to 3.56 ± 1010 (1/mol). The antigen precipitated by all five antibodies showed identical peptide maps after partial proteolytic digestion.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 109 (1983), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Chemotactic activities of circulating polymorphonuclear leukocytes (PMN) were determined in twenty patients with psoriasis and twenty healthy control persons. After serial dilution of the complement split product C5a and the formylated tripeptide f-met-Ieu-phe (FMLP), chemotaxis profiles showed that PMN migration toward both chemotaxins was significantly increased in psoriasis. In addition, PMN from psoriatic patients responded to chemotaxins at much lower concentrations compared with controls.The liberation of (lysosomal) β-glucuronidase was also determined in cytochalasin B-treated cells confronted with increased concentrations of the chemotaxins. Secretion of this marker enzyme started at lower concentrations in PMN derived from psoriatic patients.Our observations demonstrate migratory and secretory hyper-responsiveness of PMN from psoriatic patients. This may play a role in perpetuating the psoriatic tissue reaction.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 103 (1980), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The chemotactic activity of polymorphonuclear leukocytes (PMNs) directed against serum was determined in a modified Boyden chamber assay. In a total of 176 experiments PMNs and sera from healthy controls were compared with PMNs and sera from patients with psoriasis. When PMNs from patients with psoriasis were confronted with psoriatic serum greatly enhanced chemotaxis was demonstrated. Non-psoriatic PMNs in the presence of psoriatic serum showed no enhancement. However, psoriatic PMNs in the presence of normal serum were chemotactically more active compared to non-psoriatic PMNs. Heat inactivation (56°C, 30 min) reduced the chemotactic activity of all sera by nearly 50%. However in psoriatic sera the enhancement of chemotaxis was still present after heat treatment. The results indicate the presence of a functional abnormality of chemotaxis in psoriasis. This is likely to be caused by the in situ generation of chemotactically active fragments of complement. Experiments showing increased chemotactic activity of sera exposed to migrating PMNs support this concept.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 108 (1983), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Six patients suffering from widespread psoriasis were treated with 20 mg methotrexate (MTX) intramuscularly and the chemotactic activity of peripheral polymorphonuclear leucocytes (PMN) was measured before and after treatment. The modified Boyden chamber method was used and C5a, FMLP, casein and autologous fresh serum served as chemotaxins. Following MTX the chemotactic migration of PMN was inhibited by more than 50% for 2 days and slowly recovered within the following 5 days. The inhibition was present with all of the four chemotaxins used.The results demonstrate that treatment with low dose MTX in patients with psoriasis causes a profound inhibition of PMN function. These findings support the idea that PMN play an important role in the pathogenesis of psoriasis.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Es wird eine Modifikation am Henselschen Strömungscalorimeter angegeben, welche eine schnelle Veränderung der wirksamen Calorimeterwasser-Temperatur mit Hilfe einer Variierung der Zirkulationsgeschwindigkeit des Calorimeterwassers gestattet. Diese Meßtechnik eignet sich vor allem zur wiederholten Bestimmung der örtlichen Innentemperatur im instationären Zustand des Wärmedurchganges, etwa zur Untersuchung der Frage, wie sich der Temperaturgradient Körperkern-Hautoberfläche bei thermischen Reizen auf den Gesamtkörper verhält. Zur Bestimmung von Innentemperatur und örtlicher Wärmedurchgangszahl unter stationären Bedingungen sind hingegen Verfahren mit großer und möglichst gleichbleibender Zirkulationsgeschwindigkeit des Calorimeterwassers vorzuziehen.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 33 (1955), S. 833-838 
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Es wird ein rectaler Meßkopf zur Erweiterung der strömungscalorimetrischen Untersuchungen, vor allem zum Zwecke dergleichzeitigen Registrierung der Durchblutung von Rectumschleimhaut und einem Hautbezirk der Körperoberfläche angegeben. Die von uns benützte Apparatur besteht aus: 1. dem. neuen rectalen Calorimetermeßkopf und dem vonHensel angegebenen Hautmeßkopf, 2. aus einem Thermostaten mit Ausgleichsgefäß (Vascalor-Gerät nachHensel) und 3. einem Doppellinienschreiber als Registriereinrichtung. Es werden klinische Probleme angeführt, bei denen solche synchrone Durchblutungsmessungen in der Körperperipherie und in einem Gefäßgebiet des Körperkernes neue Aufschlüsse ergeben können, und es werden entsprechende Kurvenbeispiele gezeigt. Weiter wird auf den für die calorimetrische Messung von Durchblutungsgrößen entscheidenden Unterschied zwischen Wärmeabgabe und Wärmedurchgangszahl eingegangen. Dabei werden eigene Beobachtungen und Meßergebnisse mitgeteilt.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 34 (1956), S. 536-541 
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Es wird über die Bestimmung der Wärmedurchgangszahlen der Wange bei 54 Männern (151 Bestimmungen) und bei 42 Frauen (108 Bestimmungen) mit dem strömungscalorimetrischen Verfahren nachHensel berichtet. Es ergaben sich dabei deutliche Unterschiede zwischen den Geschlechtern, die sich teilweise durch die bekannte größere Ausdehnung der poikilothermen Körperschale bei der Frau erklären lassen. Weitere beobachtete Unterschiede lassen jedoch die Vermutung zu, daß dieses verschiedene Verhalten der Körperschale mit einer verschieden starken und teilweise auch anders gerichteten Reaktion der Hautdurchblutung auf thermische Reize einhergeht. Dieses verschiedene Verhalten der Hautdurchblutung bei Mann und Frau wird in Beziehung zu der verschiedenen Pathologie der Herz- und Kreislauferkrankungen der Geschlechter gesetzt. Dabei wird vermutet, daß diese physiologischen Unterschiede von Bedeutung für die Erkennung der Natursolcher Herzund Kreislauferkrankungen sein könnten, die eine eindeutige Bevorzugung eines Geschlechtes zeigen.
    Type of Medium: Electronic Resource
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