In:
FEBS Letters, Wiley, Vol. 494, No. 1-2 ( 2001-04-06), p. 125-128
Kurzfassung:
The epithelial Na + channel (ENaC) is composed of the subunits α, β, and γ [Canessa et al., Nature 367 (1994) 463–467] and typically exhibits a high affinity to amiloride [Canessa et al., Nature 361 (1993) 467–470] . When expressed in Xenopus oocytes, conflicting results were reported concerning the osmo‐sensitivity of the channel [Ji et al., Am. J. Physiol. 275 (1998) C1182–C1190; Hawayda and Subramanyam, J. Gen. Physiol. 112 (1998) 97–111; Rossier, J. Gen. Physiol. 112 (1998) 95–96]. Rat hepatocytes were the first system in which amiloride‐sensitive sodium currents in response to hypertonic stress were reported [Wehner et al., J. Gen. Physiol. 105 (1995) 507–535; Wehner et al., Physiologist 40 (1997) A‐4] . Moreover, all three ENaC subunits are expressed in these cells [Böhmer et al., Cell. Physiol. Biochem. 10 (2000) 187–194]. Here, we injected specific antisense oligonucleotides directed against α‐rENaC into single rat hepatocytes in confluent primary culture and found an inhibition of hypertonicity‐induced Na + currents by 70%. This is the first direct evidence for a role of the ENaC in cell volume regulation.
Materialart:
Online-Ressource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(01)02303-1
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2001
ZDB Id:
1460391-3
SSG:
12
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