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  • 1
    Online Resource
    Online Resource
    Single-cell RNA-sequencing atlas reveals an FABP1-dependent immunosuppressive environment in hepatocellular carcinoma
    BMJ ; 2023
    In:  Journal for ImmunoTherapy of Cancer Vol. 11, No. 11 ( 2023-11), p. e007030-
    Details
    In: Journal for ImmunoTherapy of Cancer, BMJ, Vol. 11, No. 11 ( 2023-11), p. e007030-
    Abstract: Single-cell RNA sequencing, also known as scRNA-seq, is a method profiling cell populations on an individual cell basis. It is particularly useful for more deeply understanding cell behavior in a complicated tumor microenvironment. Although several previous studies have examined scRNA-seq for hepatocellular carcinoma (HCC) tissues, no one has tested and analyzed HCC with different stages. Methods In this investigation, immune cells isolated from surrounding normal tissues and cancer tissues from 3 II-stage and 4 III-stage HCC cases were subjected to deep scRNA-seq. The analysis included 15 samples. We distinguished developmentally relevant trajectories, unique immune cell subtypes, and enriched pathways regarding differential genes. Western blot and co-immunoprecipitation were performed to demonstrate the interaction between fatty acid binding protein 1 (FABP1) and peroxisome proliferator-activated receptor gamma(PPARG). In vivo experiments were performed in a C57BL/6 mouse model of HCC established via subcutaneous injection. Results FABP1 was discovered to be overexpressed in tumor-associated macrophages (TAMs) with III-stage HCC tissues compared with II-stage HCC tissues. This finding was fully supported by immunofluorescence detection in significant amounts of HCC human samples. FABP1 deficiency in TAMs inhibited HCC progression in vitro. Mechanistically, FABP1 interacted with PPARG/CD36 in TAMs to increase fatty acid oxidation in HCC. When compared with C57BL/6 mice of the wild type, tumors in FABP1–/– mice consistently showed attenuation. The FABP1–/– group’s relative proportion of regulatory T cells and natural killer cells showed a downward trend, while dendritic cells, M1 macrophages, and B cells showed an upward trend, according to the results of mass cytometry. In further clinical translation, we found that orlistat significantly inhibited FABP1 activity, while the combination of anti-programmed cell death 1(PD-1) could synergistically treat HCC progression. Liposomes loaded with orlistat and connected with IR780 probe could further enhance the therapeutic effect of orlistat and visualize drug metabolism in vivo. Conclusions ScRNA-seq atlas revealed an FABP1-dependent immunosuppressive environment in HCC. Orlistat significantly inhibited FABP1 activity, while the combination of anti-PD-1 could synergistically treat HCC progression. This study identified new treatment targets and strategies for HCC progression, contributing to patients with advanced HCC from new perspectives.
    Type of Medium: Online Resource
    ISSN: 2051-1426
    URL: Article
    DOI: 10.1136/jitc-2023-007030
    Language: English
    Publisher: BMJ
    Publication Date: 2023
    detail.hit.zdb_id: 2719863-7
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  • 2
    Online Resource
    Online Resource
    From Immunogen to COVID-19 vaccines: Prospects for the post-pandemic era
    Elsevier BV ; 2023
    In:  Biomedicine & Pharmacotherapy Vol. 158 ( 2023-02), p. 114208-
    Details
    In: Biomedicine & Pharmacotherapy, Elsevier BV, Vol. 158 ( 2023-02), p. 114208-
    Type of Medium: Online Resource
    ISSN: 0753-3322
    URL: Article
    DOI: 10.1016/j.biopha.2022.114208
    RVK:
    XA 10000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 1501510-5
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  • 3
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    Online Resource
    Instability Monitoring and Numerical Analysis of Typical Coal Mines in Southwest China Based on DS-InSAR
    MDPI AG ; 2022
    In:  Sensors Vol. 22, No. 20 ( 2022-10-14), p. 7811-
    Details
    In: Sensors, MDPI AG, Vol. 22, No. 20 ( 2022-10-14), p. 7811-
    Abstract: Most of the coal mines in Southwest China are located in mountainous areas with high vegetation coverage, and most activities are carried out under the mountains. The deformation monitoring and mechanical behavior analysis of the mining area helps reveal the typical mountain deformation and failure mechanism caused by underground mining activities and reduce the risk of mountain collapse in the mining area. In this manuscript, a research method for mountain stability in mining areas is proposed, which combines InSAR deformation monitoring with numerical analysis. Based on the high-precision deformation information obtained by DS-InSAR and the landslide range, a three-dimensional explicit finite difference numerical analysis method was used to reconstruct the landslide model. According to the layout of the coal mining working face, the variation mechanism of overlying stratum stress and the mountain slip in the coal mining process is inverted, and the mechanism of mountain failure and instability in the mining area is analysed. Based on the sentinel data, the experiment performed time series monitoring and inversion analysis of the mountain collapse in Nayong, Guizhou, China. The results show that mining activities a certain distance from the mountain will affect mountain stability, and there are specific mechanisms. From 2015 to 2017, the stress redistribution of overlying strata above the goaf area resulted in dense longitudinal cracks in the landslide body due to coal mining. The mountain is in a continuous damage state, and the supporting force to prevent collapse continues to decrease, resulting in a gradual decrease in landslide stability. Both the time series DS-InSAR monitoring results and numerical simulation results verify the actual occurrence and development of the on-site subsidence.
    Type of Medium: Online Resource
    ISSN: 1424-8220
    URL: Article
    DOI: 10.3390/s22207811
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2052857-7
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  • 4
    Online Resource
    Online Resource
    The effects of fermentation of Qu on the digestibility and structure of waxy maize starch
    Frontiers Media SA ; 2022
    In:  Frontiers in Plant Science Vol. 13 ( 2022-8-16)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-8-16)
    Abstract: The fermentation of Qu (FQ) could efficiently produce enzymatically modified starch at a low cost. However, it is poorly understood that how FQ influences the waxy maize starch (WMS) structure and the digestion behavior. In this study, WMS was fermented by Qu at different time and starches were isolated at each time point, and its physico-chemical properties and structural parameters were determined. Results showed that the resistant starch (RS), amylose content (AC), the average particle size [D(4,3)] the ratio of peaks at 1,022/995 cm –1 , and the onset temperature of gelatinization (T o ) were increased significantly after 36 h. Conversely, the crystallinity, the values of peak viscosity (PV), breakdown (BD), gelatinization enthalpy (ΔH), and the phase transition temperature range (ΔT) were declined significantly after 36 h. It is noteworthy that smaller starch granules were appeared at 36 h, with wrinkles on the surface, and the particle size distribution was also changed from one sharp peak to bimodal. We suggested that the formation of smaller rearranged starch granules was the main reason for the pronounced increase of RS during the FQ process.
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    DOI: 10.3389/fpls.2022.984795
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2687947-5
    detail.hit.zdb_id: 2613694-6
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  • 5
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    Online Resource
    Surgical career choices of medical students in China: does gender bias play a role?
    Springer Science and Business Media LLC ; 2022
    In:  BMC Medical Education Vol. 22, No. 1 ( 2022-12)
    Details
    In: BMC Medical Education, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2022-12)
    Abstract: Gender bias in career choices has always been a matter of great concern, including in the field of medicine. This study reports on the current situation in this regard in China, including the reasons for Chinese medical students’ willingness to engage in surgical careers; investigates their accounts of gender bias; and analyzes the effect of gender bias on their surgical career choices. Methods This study invited medical students from Harbin Medical University to fill out a non-mandatory questionnaire on whether they had witnessed gender bias, their surgical career intentions, and factors influencing their career intentions. A one-way analysis of variance was used to compare the differences between continuous variables. Pearson’s chi-squared test was used to compare the differences between the categorical variables, the Kendall correlation coefficient (tau) was used to assess the correlation between the reasons rankings reported by gender, and a multiple regression analysis was conducted by logit model. Results A total of 643 students responded to the questionnaire. Of them, 63.76% expressed a willingness for a surgical career, with “interest” being a key driving factor (73.41%). Almost all respondents (96.27%) answered that there were more male leaders in the surgical departments they had rotated through or had contacted. Only a few respondents reported gender barriers influencing recruitment (32.19%). However, witnessing gender bias (recruitment of male required) was correlated to choice of surgical career ( P   〈  0.05). Females were less willing to pursue a career in surgery if they had witnessed gender barriers in surgical recruitment. Male dominance also correlated to the choice of a surgical career ( P   〈  0.1). Of the respondents, 53.19% believed that surgery was not suitable for females; among female respondents, this number was 56.12%, higher than for male respondents. When females think that the surgical profession is not suitable for them, it reduces the possibility of their pursuing a career in surgery. Conclusion Most medical students were interested in surgical care. Witnessing gender bias decreases females’ willingness to pursue a career in surgery. It is necessary to stimulate medical students’ interest in surgery when formulating strategies to promote surgical career choices, as well as to reduce gender bias in surgery; in this way, females’ surgical careers should be ensured.
    Type of Medium: Online Resource
    ISSN: 1472-6920
    URL: Article
    DOI: 10.1186/s12909-022-03453-0
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2044473-4
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  • 6
    Online Resource
    Online Resource
    The feasibility and safety of the brachial artery approach in the treatment of hepatic artery infusion chemotherapy: a retrospective study
    AME Publishing Company ; 2023
    In:  Journal of Gastrointestinal Oncology Vol. 14, No. 4 ( 2023-8), p. 1830-1836
    Details
    In: Journal of Gastrointestinal Oncology, AME Publishing Company, Vol. 14, No. 4 ( 2023-8), p. 1830-1836
    Type of Medium: Online Resource
    ISSN: 2078-6891 , 2219-679X
    URL: Journal
    URL: Article
    DOI: 10.21037/jgo
    DOI: 10.21037/jgo-23-523
    Language: Unknown
    Publisher: AME Publishing Company
    Publication Date: 2023
    detail.hit.zdb_id: 2594644-4
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  • 7
    Online Resource
    Online Resource
    A new insight into the biosynthesis, structure, and functionality of waxy maize starch under drought stress
    Wiley ; 2023
    In:  Journal of the Science of Food and Agriculture Vol. 103, No. 11 ( 2023-08-30), p. 5270-5276
    Details
    In: Journal of the Science of Food and Agriculture, Wiley, Vol. 103, No. 11 ( 2023-08-30), p. 5270-5276
    Abstract: Drought stress (DS) is the main abiotic stress that maize suffers during its whole growth period, and maize is also sensitive to DS. It had been demonstrated that DS could improve the quality of normal maize starch. However, waxy maize, which has special properties, has not been explored in depth, which limits the breeding and cultivation of waxy maize varieties and the application of waxy maize starch. Therefore, in this study, we investigated the effects of DS on the biosynthesis, structure, and functionality of waxy maize starch. RESULTS The results showed that DS decreased the expression level of SSIIb , SSIIIa , GBSSIIa , SBEI , SBEIIb , ISAII , and PUL , but increased the expression level of SSI and SBEIIa . DS did not change the average chain length of amylopectin, while increased the relative content of fa chains (RC fa ) and decreased the RC fb1 and RC fb3 . Furthermore, DS decreased the amylose content, amorphous lamellar distance d a , semi‐crystalline repeat distance, and average particle size, whereas it increased the relative crystallinity, crystalline distance d c , the content of rapidly digested starch in the uncooked system and resistant starch content in both the uncooked and cooked system. Conclusions For waxy maize, DS could raise the relative expression level of SSI and SBEIIa , thus increasing RC fa . The larger number of RC fa could create steric hindrance, which can lead to producing more resistant starch in waxy maize starch. © 2023 Society of Chemical Industry.
    Type of Medium: Online Resource
    ISSN: 0022-5142 , 1097-0010
    URL: Issue
    URL: Article
    DOI: 10.1002/jsfa.v103.11
    DOI: 10.1002/jsfa.12599
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2001807-1
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  • 8
    Online Resource
    Online Resource
    Template-based fabrication of nanowire–nanotube hybrid arrays
    IOP Publishing ; 2008
    In:  Nanotechnology Vol. 19, No. 32 ( 2008-08-13), p. 325303-
    Details
    In: Nanotechnology, IOP Publishing, Vol. 19, No. 32 ( 2008-08-13), p. 325303-
    Type of Medium: Online Resource
    ISSN: 0957-4484 , 1361-6528
    URL: Article
    DOI: 10.1088/0957-4484/19/32/325303
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 2008
    detail.hit.zdb_id: 1362365-5
    SSG: 11
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  • 9
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    Online Resource
    A new theropod dinosaur from the Lower Cretaceous Longjiang Formation of Inner Mongolia (China)
    Elsevier BV ; 2023
    In:  Cretaceous Research Vol. 151 ( 2023-11), p. 105605-
    Details
    In: Cretaceous Research, Elsevier BV, Vol. 151 ( 2023-11), p. 105605-
    Type of Medium: Online Resource
    ISSN: 0195-6671
    URL: Article
    DOI: 10.1016/j.cretres.2023.105605
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 1463187-8
    SSG: 13
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  • 10
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    Online Resource
    What constrains impoverished rural regions: A case study of Henan Province in central China
    Elsevier BV ; 2022
    In:  Habitat International Vol. 119 ( 2022-01), p. 102477-
    Details
    In: Habitat International, Elsevier BV, Vol. 119 ( 2022-01), p. 102477-
    Type of Medium: Online Resource
    ISSN: 0197-3975
    URL: Article
    DOI: 10.1016/j.habitatint.2021.102477
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 2001576-8
    SSG: 14
    SSG: 10
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