In:
American Journal of Physiology-Endocrinology and Metabolism, American Physiological Society, Vol. 283, No. 6 ( 2002-12-01), p. E1308-E1315
Kurzfassung:
We investigated the contribution of neutrophils to prostaglandin (PG)F 2α -induced luteolysis and the role of reactive oxygen species (ROS) as potential mediators of neutrophil accumulation in regressing corpora lutea of superovulated rats. On day 8of pseudopregnancy, subcutaneous injection of PGF 2α (500 μg) significantly increased rhodamine 6G-labeled leukocyte adhesion in luteal venules, as observed by intravital microscopy. Neutrophil accumulation was confirmed by significantly increased myeloperoxidase (MPO) activity. Pretreatment with anti-leukocyte antibody (CD18-directed monoclonal antibody, WT-3) significantly inhibited the PGF 2α -induced increases in adherent leukocytes and MPO activity. Anti-leukocyte antibody also maintained serum progesterone concentrations. Pretreatment with oxygen free radical scavengers, superoxide dismutase (50,000 U/kg) and catalase (90,000 U/kg), also attenuated these PGF 2α -induced alterations. Corpora lutea preloaded with dichlorodihydrofluorescein diacetate succinimidyl ester, a fluorescent indicator for determining intracellular ROS generation, exhibited an increase in fluorescence after PGF 2α treatment. These findings suggest that leukocyte-endothelium interactions mediated by ROS generation are important in PGF 2α -induced luteolysis in rats.
Materialart:
Online-Ressource
ISSN:
0193-1849
,
1522-1555
DOI:
10.1152/ajpendo.00240.2002
Sprache:
Englisch
Verlag:
American Physiological Society
Publikationsdatum:
2002
ZDB Id:
1477331-4
SSG:
12
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