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  • Guo, Mingxiao  (8)
  • Lu, Chunlei  (8)
  • 1
    Online-Ressource
    Online-Ressource
    Intestinal Conditioning After Cardiac Arrest: The Use of Normothermic Extracorporeal Membrane Oxygenation in the Non‐Heart‐Beating Animal Model
    Wiley ; 2016
    In:  Artificial Organs Vol. 40, No. 8 ( 2016-08), p. 738-745
    Details
    In: Artificial Organs, Wiley, Vol. 40, No. 8 ( 2016-08), p. 738-745
    Kurzfassung: The effect of normothermic extracorporeal membrane oxygenation (NECMO) on small bowel preservation in a clinically relevant large animal model of expected donation after cardiac death (eDCD) was evaluated. Thirty domestic crossbred donor pigs were divided into five groups. The first group served as the live donation (LD) group, the second group served as the donation after cardiac death (DCD) group, and the remaining were further assigned into three subgroups: E1 group (1 h NECMO support), E3 group (3 h NECMO support), and E5 group (5 h NECMO support). Pathology, electron microscopy, energy metabolism, cell apoptosis, and tight junction (TJ) protein expression level of intestinal mucosa and the level of plasma d ‐lactic acid were evaluated in normal, cardiac death and at the end of extracorporeal support, respectively. The mean arterial pressure and PaO 2 were maintained over 60 and 267 mm Hg during NECMO support, respectively. One hour of extracorporeal support could improve the energy status in intestines of the DCD group. Although the histologic damage and apoptosis of the E1 group had no significant difference with those of the LD and DCD groups ( P   〉  0.05), the levels of intestinal mucosa TJ protein decreased ( P   〈  0.05), and plasma d ‐lactic acid increased progressively ( P   〈  0.05). With the extension of extracorporeal support, the degree of intestinal mucosa damage and intestinal permeability gradually increased, as well as the content of adenosine triphosphate in intestinal mucosa. The normothermic extracorporeal support for 1 h in DCD is beneficial for improving the energy status and viability of the bowel. However, the integrity of intestinal mucosa was destroyed gradually as extracorporeal support time went by. And the activation of intestinal epithelial apoptosis and hyperoxia might be the factors that lead to intestinal mucosa injury.
    Materialart: Online-Ressource
    ISSN: 0160-564X , 1525-1594
    URL: Issue
    URL: Article
    DOI: 10.1111/aor.2016.40.issue-8
    DOI: 10.1111/aor.12691
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2016
    ZDB Id: 2003825-2
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
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    Normothermic extracorporeal membrane oxygenation support: Improving the function of intestinal grafts obtained from cardiac death donors
    Wiley ; 2020
    In:  Artificial Organs Vol. 44, No. 10 ( 2020-10), p. 1098-1106
    Details
    In: Artificial Organs, Wiley, Vol. 44, No. 10 ( 2020-10), p. 1098-1106
    Kurzfassung: Extracorporeal membrane oxygenation (ECMO) could ameliorate the energy status and viability of bowel grafts from cardiac death donors. However, the function of these grafts after transplantation is not clear. The purpose of the study was to evaluate the early function of intestinal grafts after transplantation from expected cardiac death donors supported with normothermic extracorporeal support using a porcine allogeneic orthotopic segmental small bowel transplantation model. Eighteen domestic crossbred donor pigs were assigned to living donation (LD), donation after cardiac death (DCD), and ECMO groups. In the LD group, small bowels were harvested and preserved immediately in cold storage. In the other two groups, the donor pigs received conventional rapid recovery treatment or 1‐hour normothermic extracorporeal support after 10‐minutes expected cardiac arrest. Subsequently, the small bowels were removed and preserved in cold storage. After 5‐6 hours of preservation, small bowel grafts were transplanted into the recipient pigs that underwent enterectomy. The pathology and electron microscopy results, cell apoptosis rate, tight junction protein expression level in the intestinal mucosa, and plasma endotoxin level were evaluated after transplantation. All grafts functioned on the basis of the maltose absorption test results at day 7 after transplantation. There were no significant differences in the morphological changes in the intestinal mucosa among the three groups at day 7 after transplantation. The cell apoptosis rate and plasma endotoxin level in the ECMO group did not differ significantly than those in the LD group, but were evidently lower than those in the DCD group ( P   〈  .001). The intestinal absorptive function improved significantly in the ECMO group in contrast with that in the DCD group ( P   〈  .001). Short‐term ECMO intervention can alleviate ischemia–reperfusion injuries in intestinal grafts and improve intestinal absorptive function in the early stage after transplantation. Reducing caspase‐3 protein expression and cell apoptosis in the intestinal mucosa may be one of the protective mechanisms of ECMO intervention.
    Materialart: Online-Ressource
    ISSN: 0160-564X , 1525-1594
    URL: Issue
    URL: Article
    DOI: 10.1111/aor.v44.10
    DOI: 10.1111/aor.13697
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2020
    ZDB Id: 2003825-2
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
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    Characterization of the withdrawal phase in a swine controlled intestinal donation after circulatory death model
    University of Toronto Libraries - UOTL ; 2014
    In:  Clinical & Investigative Medicine Vol. 37, No. 5 ( 2014-10-04), p. 331-
    Details
    In: Clinical & Investigative Medicine, University of Toronto Libraries - UOTL, Vol. 37, No. 5 ( 2014-10-04), p. 331-
    Kurzfassung: Purpose: Transplantation of donation after cardiac death (DCD) intestine has higher rates of organ failure and complications. Fortunately, this is less grievous in a subclass of DCD called controlled (CDCD), those with irreversible but incomplete brain injury. The aim of the paper is to establish a CDCD porcine model which is closely mimicking human CDCD scenario, and investigate the physiologic changes from withdrawal of ventilatory support to circulatory arrest. Method: Ten domestic crossbred pigs were anesthetized and ventilated with room air. Once all baseline data was taken, atracurium besilate (0.9 mg/kg, 3×ED95) was administered and the ventilator was discontinued while the animal was under deep anesthesia to establish the porcine CDCD model. Meanwhile, heparin (150~200 U/kg) was administered after discontinuation of the ventilator. The time to death and the changes of arterial blood gases and hemodynamic parameters were monitored every 5 minutes until circulatory arrest. In addition, histopathology, ultrastructures (via electron microscope) and expression of tight junction proteins of intestinal mucosa were observed at the baseline and the time of death. Result: The mean time to death was approximately (21.8±3.12 min. Within 5 minutes of removal of the ventilator, there was a hyperdynamic period. Systolic blood pressure and heart rate quickly increased to 118.5±10.4 mmHg and 108.2±4.94 bpm, respectively. Blood pressure and heart rate then reduced rapidly until circulatory arrest. Moreover, the PaO2 quickly dropped to 17.4±3.13 mmHg, the blood gases throughout the apneic time showed a rapid hypercapnia and acidosis. In addition, warm ischemia damaged intestinal mucosa and reduced TJ proteins expression. Conclusion: A new swine CDCD model, simulating three stages of “withdrawal of ventilation, systemic anticoagulation and determination of death”, which closely mimics the human DCD scenario and can thus be used in studies related to organ transplantation, was successfully established.
    Materialart: Online-Ressource
    ISSN: 1488-2353
    URL: Article
    DOI: 10.25011/cim.v37i5.22013
    Sprache: Unbekannt
    Verlag: University of Toronto Libraries - UOTL
    Publikationsdatum: 2014
    ZDB Id: 2067562-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
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    Identification of potential biomarkers for colorectal cancer by clinical database analysis and Kaplan–Meier curves analysis
    Ovid Technologies (Wolters Kluwer Health) ; 2023
    In:  Medicine Vol. 102, No. 6 ( 2023-02-10), p. e32877-
    Details
    In: Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 102, No. 6 ( 2023-02-10), p. e32877-
    Kurzfassung: This study aimed to explore critical genes as potential biomarkers for the diagnosis and prognosis of colorectal cancer (CRC) for clinical utility. To identify and screen candidate genes involved in CRC carcinogenesis and disease progression, we downloaded microarray datasets GSE89076, GSE73360, and GSE32323 from the GEO database identified differentially expressed genes (DEGs), and performed a functional enrichment analysis. A protein-protein interaction network was constructed, and correlated module analysis was performed using STRING and Cytoscape. The Kaplan–Meier survival curve shows the survival of the hub genes. The expression of cyclin-dependent kinase (CDK1), cyclin B1 (CCNB1), and PCNA in tissues and changes in tumor grade were analyzed. A total of 329 DEGs were identified, including 264 upregulated and 65 downregulated genes. The functions and pathways of DEGs include the mitotic cell cycle, poly(A) RNA binding replication, ATP binding, DNA replication, ribosome biogenesis in eukaryotes, and RNA transport. Forty-seven Hub genes were identified, and biological process analysis showed that these genes were mainly enriched in cell cycle and DNA replication. Patients with mutations in CDK1, PCNA, and CCNB1 had poorer survival rates. CDK1, PCNA, and CCNB1 were significantly overexpressed in the tumor tissues. The expression of CDK1 and CCNB1 gradually decreased with increasing tumor grade. CDK1, CCNB1, and PCNA can be used as potential markers for the diagnosis and prognosis of CRC. These genes are overexpressed in colon cancer tissues and are associated with low survival rates in CRC patients.
    Materialart: Online-Ressource
    ISSN: 0025-7974
    URL: Article
    DOI: 10.1097/MD.0000000000032877
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2023
    ZDB Id: 2049818-4
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
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    Prognostic modeling of overall survival and analysis of K-M survival curves in patients with primary colon cancer: A SEER-based study
    Ovid Technologies (Wolters Kluwer Health) ; 2023
    In:  Medicine Vol. 102, No. 23 ( 2023-06-09), p. e33902-
    Details
    In: Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 102, No. 23 ( 2023-06-09), p. e33902-
    Kurzfassung: This study aimed to establish a validated prognostic survival column line chart by analyzing data from patients with colon cancer (CC) in the SEER database. The nomogram proposed in this study was based on the retrospective data of patients diagnosed with CC in the SEER database from 1975 to 2015. Randomly divided into training and validation sets, the nomogram was constructed using the Cox model, and the discriminatory power of the nomogram and its predictive accuracy were determined using the consistency index and associated calibration curves. In a multifactorial analysis of the main cohort, the independent factors for survival were age, sex, race, tumor stage, and tumor grade, all of which were included in the nomogram and were prognostic factors for patients with CC ( P   〈  .05). The calibration curve of the survival probability showed good agreement between the prediction of the nomogram and the actual observation. The validation calibration curve showed good correlation and agreement between predicted and observed values. Multifactorial analysis showed that the factors affecting the prognosis of patients with CC included age, sex, race, tumor-node-metastasis stage, and tumor pathological stage. The nomogram prediction model proposed in this study has high accuracy and can provide more accurate prognostic prediction and relevant reference values for assessing the postoperative survival of CC patients and guiding clinical decision-making.
    Materialart: Online-Ressource
    ISSN: 0025-7974
    URL: Article
    DOI: 10.1097/MD.0000000000033902
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2023
    ZDB Id: 2049818-4
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
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    Characterization of the intestinal graft in a swine hypotensive after brain death model
    FapUNIFESP (SciELO) ; 2019
    In:  Acta Cirurgica Brasileira Vol. 34, No. 11 ( 2019-11)
    Details
    In: Acta Cirurgica Brasileira, FapUNIFESP (SciELO), Vol. 34, No. 11 ( 2019-11)
    Materialart: Online-Ressource
    ISSN: 1678-2674 , 0102-8650
    URL: Article
    DOI: 10.1590/s0102-865020190110000007
    Sprache: Englisch
    Verlag: FapUNIFESP (SciELO)
    Publikationsdatum: 2019
    ZDB Id: 2012156-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
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    Lifor Solution: An Alternative Preservation Solution in Small Bowel Transplantation
    Hindawi Limited ; 2016
    In:  Gastroenterology Research and Practice Vol. 2016 ( 2016), p. 1-6
    Details
    In: Gastroenterology Research and Practice, Hindawi Limited, Vol. 2016 ( 2016), p. 1-6
    Kurzfassung: Background and Objectives . The intestinal mucosa is extremely sensitive to ischemia. Better intestinal preservation is the first step to improve the results of intestinal transplantation. The aim of the study is to investigate the effect of cold Lifor solution on preservation of swine small bowel. Methods . Swine ileum segments (200 cm) were allotransplanted heterotopically after 9-hour cold storage with UW solution (group 1, n = 6 ), with Lifor solution (group 2, n = 6 ), or without storage (group 3, n = 6 ), respectively. After cold storage, mucosal adenosine triphosphate (ATP) concentrations and histopathologic analysis after preservation were performed. At day 7 after the transplantation, intestinal absorptive function was also observed. Results . After 9 h cold preservation, pathological changes, the content of ATP in the intestinal mucosa, and the intestinal absorptive function after transplantation in group 2 were similar to those of group 1. Conclusion . The effect of cold storage of swine small bowel with Lifor solution is similar to that of UW solution. It may provide additional rationale for further exploration of Lifor as an alternative preservation solution in small bowel transplantation.
    Materialart: Online-Ressource
    ISSN: 1687-6121 , 1687-630X
    URL: Article
    DOI: 10.1155/2016/3925751
    Sprache: Englisch
    Verlag: Hindawi Limited
    Publikationsdatum: 2016
    ZDB Id: 2435460-0
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
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    Early Intestinal Rehabilitation Therapy Ameliorates Intestinal Adaptation in Children with Short Bowel Syndrome: The Long-Term Outcome
    SAGE Publications ; 2016
    In:  The American Surgeon Vol. 82, No. 12 ( 2016-12), p. 1215-1220
    Details
    In: The American Surgeon, SAGE Publications, Vol. 82, No. 12 ( 2016-12), p. 1215-1220
    Kurzfassung: In the management of short bowel syndrome (SBS), the benefits of treatment with growth hormone (GH), glutamine, and enteral nutrition (EN) on intestinal adaptation among children patients is still controversial. The aim of present study is to determine whether GH, glutamine, and EN have positive effect on intestinal adaptation in children with SBS. Sixteen children with SBS (small bowel remnant length, 56.75 ± 8.09 cm; mean ± SE) were treated with GH (0.05 mg/kg/d), glutamine (0.45 mg/kg/d), plus EN-enriched fiber diet for four weeks. After four weeks of treatment, patients were discharged home; GH was discontinued, but the EN with glutamine was continued. Repeated treatment was performed if there were lose weight, dysplasia, or severe diarrhea. All patients completed the treatment. Body weight, intestinal absorptive capacity, and plasma levels of proteins were significantly improved after complete treatment, without any major adverse effects. On follow-up, no death was reported. Treatment with GH, glutamine, and EN in early stage significantly improved intestinal adaptation in pediatric patients with SBS. Furthermore, the positive effect of the treatment does not seem to be sustained once GH discontinued until the residual intestinal adaptation reaches its maximum.
    Materialart: Online-Ressource
    ISSN: 0003-1348 , 1555-9823
    URL: Article
    DOI: 10.1177/000313481608201229
    Sprache: Englisch
    Verlag: SAGE Publications
    Publikationsdatum: 2016
    Standort Signatur Einschränkungen Verfügbarkeit
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