In:
APMIS, Wiley, Vol. 122, No. 9 ( 2014-09), p. 772-780
Abstract:
Aquaporins ( AQP ) are channels in the cell membrane that mainly facilitate a passive transport of water. In the eye, AQP s are expressed in the ciliary body and retina and may contribute to the pathogenesis of glaucoma and optic neuropathy. We investigated the expression of AQP 1, AQP 3, AQP 4, AQP 5, AQP 7 and AQP 9 in human glaucoma eyes compared with normal eyes. Nine glaucoma eyes were examined. Of these, three eyes were diagnosed with primary open angle glaucoma; three eyes had neovascular glaucoma; and three eyes had chronic angle‐closure glaucoma. Six eyes with normal intraocular pressure and without glaucoma were used as control. Immunohistochemistry was performed using antibodies against AQP 1, AQP 3, AQP 4, AQP 5, AQP 7 and AQP 9. For each specimen, optical densities of immunoprecipitates were measured using Photoshop and the staining intensities were calculated. Immunostaining showed labelling of AQP 7 and AQP 9 in the nonpigmented ciliary epithelium and the staining intensities were significantly decreased in glaucoma eyes (p = 0.003; p = 0.018). AQP 7 expression in the Müller cell endfeet was increased (p = 0.046), and AQP 9 labelling of the retinal ganglion cells ( RGC ) showed decreased intensity (p = 0.037). No difference in AQP 1, AQP 4 and AQP 9 expression was found in the optic nerve fibres. This study is the first investigating AQP s in human glaucoma eyes. We found a reduced expression of AQP 9 in the retinal ganglion cells of glaucoma eyes. Glaucoma also induced increased AQP 7 expression in the Müller cell endfeet. In the ciliary body of glaucoma eyes, the expression of AQP 7 and AQP 9 was reduced. Therefore, the expression of AQP s seems to play a role in glaucoma.
Type of Medium:
Online Resource
ISSN:
0903-4641
,
1600-0463
DOI:
10.1111/apm.2014.122.issue-9
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2098213-6
SSG:
12
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