In:
eLife, eLife Sciences Publications, Ltd, Vol. 8 ( 2019-06-17)
Kurzfassung:
The organisation of mammalian genomes into loops and topologically associating domains (TADs) contributes to chromatin structure, gene expression and recombination. TADs and many loops are formed by cohesin and positioned by CTCF. In proliferating cells, cohesin also mediates sister chromatid cohesion, which is essential for chromosome segregation. Current models of chromatin folding and cohesion are based on assumptions of how many cohesin and CTCF molecules organise the genome. Here we have measured absolute copy numbers and dynamics of cohesin, CTCF, NIPBL, WAPL and sororin by mass spectrometry, fluorescence-correlation spectroscopy and fluorescence recovery after photobleaching in HeLa cells. In G1-phase, there are ~250,000 nuclear cohesin complexes, of which ~ 160,000 are chromatin-bound. Comparison with chromatin immunoprecipitation-sequencing data implies that some genomic cohesin and CTCF enrichment sites are unoccupied in single cells at any one time. We discuss the implications of these findings for how cohesin can contribute to genome organisation and cohesion.
Materialart:
Online-Ressource
ISSN:
2050-084X
DOI:
10.7554/eLife.46269.001
DOI:
10.7554/eLife.46269.002
DOI:
10.7554/eLife.46269.007
DOI:
10.7554/eLife.46269.003
DOI:
10.7554/eLife.46269.004
DOI:
10.7554/eLife.46269.005
DOI:
10.7554/eLife.46269.006
DOI:
10.7554/eLife.46269.008
DOI:
10.7554/eLife.46269.009
DOI:
10.7554/eLife.46269.011
DOI:
10.7554/eLife.46269.010
DOI:
10.7554/eLife.46269.012
DOI:
10.7554/eLife.46269.013
DOI:
10.7554/eLife.46269.014
DOI:
10.7554/eLife.46269.015
DOI:
10.7554/eLife.46269.016
DOI:
10.7554/eLife.46269.017
DOI:
10.7554/eLife.46269.018
DOI:
10.7554/eLife.46269.019
DOI:
10.7554/eLife.46269.020
DOI:
10.7554/eLife.46269.021
DOI:
10.7554/eLife.46269.022
DOI:
10.7554/eLife.46269.023
DOI:
10.7554/eLife.46269.024
DOI:
10.7554/eLife.46269.025
DOI:
10.7554/eLife.46269.026
DOI:
10.7554/eLife.46269.032
DOI:
10.7554/eLife.46269.033
Sprache:
Englisch
Verlag:
eLife Sciences Publications, Ltd
Publikationsdatum:
2019
ZDB Id:
2687154-3
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