In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_10 ( 2014-10-01), p. 3375-3383
Kurzfassung:
Three novel moderately anaerobic, thermophilic, rod-shaped bacterial strains, KY38 T , KY46 T and KA13 T , were isolated from shellfish collected on the Pacific coastline of Enoshima, Japan. Phylogenetic analysis of the 16S rRNA gene sequences indicated that these bacteria belong to the genus Symbiobacterium , sharing sequence similarities of 97.8 % (KY38 T ), 96.4 % (KY46 T ) and 93.3 % (KA13 T ) with the type strain of Symbiobacterium thermophilum , the only species of the genus with a validly published name. These isolates reduced nitrate and grew optimally at 55–60 °C. Strains KY38 T and KA13 T formed endospore-like structures in the terminal or subterminal part of their cells at low frequencies. Genomic DNA G+C contents were 68.8 (KY38 T ), 67.2 (KY46 T ) and 67.1 (KA13 T ) mol%. The isolates all presented the predominant menaquinone MK-6, major fatty acids iso-C 15 : 0 , C 16 : 0 and iso-C 17 : 0 and the major polar lipids phosphatidylglycerol, phosphatidylethanolamine and unknown glycol-containing phospholipids. On the basis of their morphological, physiological and phylogenetic properties, strains KY38 T , KY46 T and KA13 T represent three novel species, for which the names Symbiobacterium ostreiconchae sp. nov. (type strain KY38 T = DSM 27624 T = KCTC 4567 T = JCM 15048 T ), Symbiobacterium turbinis sp. nov. (type strain KY46 T = DSM 27625 T = KCTC 4568 T = JCM 15996 T ) and Symbiobacterium terraclitae sp. nov. (type strain KA13 T = DSM 27138 T = KCTC 4569 T = JCM 15997 T ) are proposed. An emended description of the genus Symbiobacterium is also presented. The phylogenetic distinctiveness of the genus Symbiobacterium indicates its affiliation with a novel family, for which the name Symbiobacteriaceae fam. nov. is proposed.
Materialart:
Online-Ressource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.063750-0
Sprache:
Englisch
Verlag:
Microbiology Society
Publikationsdatum:
2014
ZDB Id:
215062-1
ZDB Id:
2056611-6
SSG:
12
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