In:
ABI Genetika, National Library of Serbia, Vol. 54, No. 3 ( 2022), p. 1049-1068
Kurzfassung:
Identifying gene interactions and markers associated with physiological
traits, especially at later stages of grain filling, can help develop
effective breeding methodology in wheat crop. Six generations (P1, P2, F1,
F2, BC1P1 and BC1P2) of four different spring wheat crosses
(drought-responsive x drought susceptible) and F3 generation of a single
cross, i.e., MACS6272 x UP2828 were phenotyped and genotyped to decipher
gene action and associated markers. Ample variation in canopy temperature
depression (CTD - 2.6 - 5.6?C), chlorophyll content by SPAD (39.6 - 51.3),
relative water content (RWC - 51.5 - 75.4 %), grain filling period (GFP -
61.1 - 80.1 days), 100 seed weight (3.7 - 5.5 grams), harvest index (HI -
25.8 - 46.2 %), biological yield (BY - 35.5 - 89.8 grams) and grain yield
(GY - 13.4 - 36.5 grams) per plant were observed in six generations. GY
positively correlated with CTD, SPAD, 100SW, BY and HI (0.08* - 0.85**). BY
had the maximum direct (0.82) and indirect effect via other traits on GY.
Significant non-additive epistatic interactions (j & l) and duplicate gene
action were found for most traits except GFP and 100SW. Seven different SSR
markers associated with CTD, SPAD, NDVI, RWC, 100SW, and explained
phenotypic variation (PVE) ranging from 10.1% to 18.4%, with marker Xcfd35
explaining highest PVE for RWC. The identified candidate genes (in silico)
belonged to transmembrane proteins (Xcfd32, Xcfd50), nucleic acid binding
domains (Xbarc124, Xgwm484) and having enzymatic activity (Xcfd35, Xwmc47,
Xwmc728) important for abiotic stress tolerance. Complex inheritance
deciphered by six generations indicated delaying the selection to later
stages of segregation so that useful transgressive segregants can be
selected for improving grain yields in wheat.
Materialart:
Online-Ressource
ISSN:
0534-0012
,
1820-6069
DOI:
10.2298/GENSR2203049G
Sprache:
Englisch
Verlag:
National Library of Serbia
Publikationsdatum:
2022
ZDB Id:
2585955-9
SSG:
12
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