In:
Crop Science, Wiley, Vol. 54, No. 3 ( 2014-05), p. 873-881
Kurzfassung:
Phenotyping of a mapping population is usually the bottleneck that limits the size of the mapping population and mapping resolution. A homogeneous population mapping approach was used for mapping maize ( Zea mays L.) gametophytic factor 1 ( ga1 ) that could completely eliminate phenotyping during the mapping process. The strong allele of maize ga1 ( Ga1‐S ), from popcorn inbred line SDGa25, showed a 100% cross‐incompatibility with the majority of Chinese dent and flint maize. A homogeneous mapping population was developed by crossing an (SDGa25/Jing66) F 1 male back to an SDGa25 female. During the pollination process, pollen grains of ga1 were completely excluded from fertilization, and a homogeneous BC 1 F 1 (SDGa25//SDGa25/Jing66) population was created in which only the Ga1‐S / Ga1‐S genotype existed, making phenotyping unnecessary. A total of 2245 individuals of this population were genotyped with SD9 and SD12 markers and 20 recombinants were identified. The Ga1‐S locus was quickly delineated to a 100 Kb region between markers dCS1 and insertion deletion ID7 at position 9,491,422 and 9,591,946 bp based on the B73 RefGen_v2 sequence . By marker‐assisted selection, Ga1‐S was introgressed into parental lines of an elite white waxy maize hybrid by six generations of backcrossing and one generation of selfing. The homozygous Ga1‐S / Ga1‐S hybrid showed full cross‐incompatibility to ga1 maize. The Ga1‐S allele could be used as a biological reproductive barrier in reducing cross‐pollination between different types of maize such as waxy and non‐waxy, genetically modified (GM) and non‐GM maize.
Materialart:
Online-Ressource
ISSN:
0011-183X
,
1435-0653
DOI:
10.2135/cropsci2013.09.0598
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2014
ZDB Id:
1480918-7
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