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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 53 (1998), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Allergy 55 (2000), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Transendothelial migration of cells to sites of inflammation is a hallmark of the allergic reaction. The adhesion cascade involves the initial expression of the adhesion molecule E-selectin on endothelial cells. The aim of the study was to determine the efficacy of a 30-min preincubation of the glucocorticosteroids (GCS) fluticasone, prednisolone, and fluocortin butyl on allergen- and interleukin (IL)-1β-induced E-selectin expression in allergic rhinitis. Methods: Freshly taken nasal inferior turbinate mucosa of 19 subjects with allergic rhinitis was cut into small cubes and preincubated for 30 min with prednisolone (n=6), fluticasone (n=5), and fluocortin butyl (n=3) in different concentrations, followed by allergen exposure at a concentration of 1000 BU/ml for 1 and 2 h. Additionally, fluticasone-preincubated tissues were exposed to recombinant human rhIL-1β (n=5) at a concentration of 2 pg/ml. The expression of E-selectin was assessed by immunohistochemistry (APAAP technique) and computerized image evaluation. Results: In this model, E-selectin expression was significantly upregulated by allergen and rhIL-1β within 1 and 2 h. After 30-min preincubation with prednisolone and fluocortin butyl at drug concentrations of 10−8 mol/l, we found a significant (≥50%) reduction of the E-selectin expression after 1 and 2 h. Allergen-induced E-selectin expression was nearly abolished at concentrations of 10−5 (prednisolone) and 10−4 mol/l (fluocortin butyl). Fluticasone significantly inhibited E-selectin expression by ≥50% at concentrations of 10−14 and 10−12 mol/l after 1 and 2 h, and abolished E-selectin induction at concentrations of 10−12 and 10−10 mol/l, respectively. Exposure of mucosal cubes to rhIL-1β (n=5) also induced rapid upregulation of E-selectin expression, an effect which could be only partially suppressed by fluticasone preincubation at concentrations of 10−10 mol/l. Conclusions: Allergen-induced E-selectin expression is significantly and rapidly inhibited by GCS preincubation, fluticasone being more potent than prednisolone and fluocortin butyl. We suggest that this rapid effect is mainly indirect, possibly by inhibition of allergen-induced cytokine release.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Munksgaard International Publishers
    Allergy 58 (2003), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Polyposis, asthma, aspirin-intolerance and aspirin-triad are mostly accompanied with eosinophilia of mucosal airways. Chemotactic cytokines, the CC-chemokines regulated on activation, normal T-cell expressed (RANTES), eotaxin, and eotaxin-2 activate and attract eosinophilic leukocytes to the site of inflammation. This points to the implication of CC-chemokines in eosinophilia of nasal tissue of these diseases.Methods:  Therefore, nasal polypous tissue specimens of patients suffering from chronic nasal polypous sinusitis (NP), intrinsic asthma (ATA), aspirin-intolerance (AINA), and aspirin-triad (TRIAD) were investigated. The amount of mRNA and protein of CC-chemokines was analyzed using semi-quantitative reverse transcriptase polymerase chain reaction and chemokine-specific enzyme-immuno-assays. The patterns of CC-chemokines were compared.Results:  The mRNA-expression as well as protein synthesis of CC-chemokines was quantified in all tissues investigated. The expression of RANTES-mRNA in NP, ATA, AINA, and TRIAD (averaging 148–324%d-glyceraldehyde-3-phosphate dehydrogenase) and protein synthesis (0.13–0.15 ng/mg tissue weight) did not differ significantly. But the protein synthesis of eotaxin- and eotxin-2-mRNA was significantly (P 〈 0.05) higher in TRIAD (3.3 pg/mg and3.4 ng/mg tissue weight) (4 ng/mg tissue weight), than in NP, ATA, or AINA (1.8 pg/mg and 2.1 ng/mg, 2.1 pg/mg and 1.6 ng/mg, or 1.7 pg/mg and2.2 ng/mg tissue weight, respectively).Conclusion:  Patients suffering from TRIAD in association with tissue eosinophilia were characterized by elevated eotaxin and eotaxin-2 mRNA-expression as well as protein-synthesis. This pointed to the implication of eotaxins and RANTES in eosinophilia-associated diseases. Further studies will have to prove, whether the analysis of these chemokines might improve the diagnosis of eosinophilia associated polyposis and initiate the development of new therapeutic strategies.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Munksgaard International Publishers
    Allergy 57 (2002), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: To gain insight into the mechanisms responsible for tissue neutrophil immigration in sinusitis, primary nasal fibroblasts are analyzed for synthesizing and delivering neutrophil chemokines.Methods: Primary nasal fibroblast cell culture was treated with tumor necrosis factor (TNF)-α concentrations of 20 and 200 ng/ml for 2, 8, 24 and 72 h. Chemokine concentrations in supernatants were determined by enzyme-linked immunoassay (ELISA) and chemokine mRNA expression in fibroblasts was measured by reverse transcriptase polymerase chain reaction (RT-PCR). Biological chemotactic activity was identified by three-step high-performance liquid chromatography (HPLC) and by bioassay measuring neutrophil chemotaxis in a single Boyden chamber system.Results: Interleukin (IL)-8 and growth-related oncogene (GRO)-α were induced in nasal fibroblast culture by proinflammatory stimulus. After 24 h of stimulation neutrophil chemotactic activity only was detected for IL-8. Granulocyte chemotactic protein (GCP)-2 mRNA was already significantly up-regulated after 2 h of stimulation.Conclusion: Induction of IL-8 protein dominates chemokine synthesis 24 and 72 h after stimulation, whereas induction of GCP-2 mRNA seems to have a role in the early phase after 2 h of exposition with TNF-α.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1433-0458
    Keywords: Schlüsselwörter Tinnitus ; Lidocain-Therapie ; Tocainid-Therapie ; Tinnitussuppression ; Key words Treatment of tinnitus ; Intravenous lidocaine therapy ; Tocainide therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The efficacy of intravenous lidocaine therapy in patients suffering form severe tinnitus aurium has been reported for many years although pharmacological mechanisms for its use are not fully understood. In order to evaluate the effectiveness of lidocaine therapy in the treatment of tinnitus we performed a retrospective study on 77 patients suffering from tinnitus who were treated between 1993 and 1995. All patients were given a test dose of lidocaine after a saline placebo infusion. Suppression of tinnitus was classified according to a visual analogue scale. Our results showed that 19 of the 77 patients investigated experienced different degrees of reduced tinnitus. Fourteen of these latter patients also were treated with oral tocainide 3×400 mg/day. Treatment was stopped in 13 of the patients because of side-effects or an insufficient effect on tinnitus. Our findings suggest that lidocaine and tocainide do not have a significant role in pharmacological treatment of tinnitus except in certain cases of long-standing severe tinnitus.
    Notes: Zusammenfassung Um die Wirksamkeit von Lidocain und Tocainid auf Tinnitus aurium zu überprüfen, untersuchten wir retrospektiv von 1993 bis Mai 1995 77 Patienten. Während des stationären Aufenthalts erhielten die Patienten eine Testdosis Lidocain i.v. und bei positivem Ansprechen auf die Testdosis eine sich anschließende medikamentöse Therapie mit Tocainid, einem oral verabreichbaren Strukturanalogon des Lidocains. Die tinnitussupprimierende Wirkung wurde entsprechend den subjektiven Angaben und anhand einer visuellen Analogskala klassifiziert. Unsere Ergebnisse zeigen, daß von 77 Patienten nur 19 Patienten eine Tinnitussuppression unterschiedlicher Ausprägung angaben. Von ihnen wählten 14 Patienten eine weiterführende Therapie mit Tocainid (3×400 mg/Tag); 5 Patienten lehnten die Therapie wegen möglicher Nebenwirkungen ab; 3 Monate nach der Verordnung von Tocainid hatten 10 Patienten das Medikament wegen Nebenwirkungen und 3 Patienten wegen mangelnder Wirkung abgesetzt. Wir schätzen die therapeutische Erfolgsquote für die Behandlung des Tinnitus aurium mit Lidocain bedeutend geringer ein als dies im Schrifttum der Fall ist. Das oral anwendbare Stukturanalogon Tocainid kommt wegen der hohen Rate an Nebenwirkungen und nicht ausreichender Tinnitussuppression allenfalls in besonderen Einzelfällen als Behandlungsmöglichkeit in Frage.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2014-09-20
    Print ISSN: 1525-2167
    Electronic ISSN: 1532-2114
    Topics: Medicine
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  • 8
    Publication Date: 2017-04-20
    Description: Aims Cancer of the major salivary glands comprises a morphologically diverse group of rare tumours of largely unknown cause. Epithelial–mesenchymal transition (EMT) has been shown to play a significant prognostic role in various human cancers. The aim was to assess the expression of EMT markers in different histological subtypes of parotid gland cancer (PGC) and analyse their prognostic value. Methods We examined 94 PGC samples (13 histological subtypes) for the expression of MIB-1, epithelial cadherin (E-cadherin), β-catenin, vimentin and cytokeratin 8/18 (CK8/18) by means of immunohistochemistry. The experimental findings were correlated with clinicopathological and survival parameters. Results We detected all analysed EMT and proliferation markers in specifically different constellations within the examined histological subtypes of PGC. We found high epithelial marker expressions (CK8/18, E-cadherin, membranous β-catenin) only in a distinct variety of carcinomas. A high proliferation rate (high MIB-1 expression) as well as a combination of high CK8/18 and low vimentin expression was associated with a significantly worse survival. Conclusions Our findings indicate that activation of the EMT pathway is a relevant explanation for tumour progression in individual histological subtypes of malignant parotid gland lesions, but by far not in all. Evidence of EMT activation in PGC cannot be seen as an isolated prognostic factor.
    Keywords: Head and neck cancer
    Print ISSN: 0021-9746
    Electronic ISSN: 1472-4146
    Topics: Medicine
    Published by BMJ Publishing Group
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