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  • 1
    Online Resource
    Online Resource
    Amsterdam :Elsevier Science & Technology,
    Keywords: Isoelectric focusing. ; Liquid scintillation counting. ; Microbiology -- Technique. ; Electronic books.
    Description / Table of Contents: Techniques of sample preraration for Liquid Scintillation Counting+Isoelectric Focusing.
    Type of Medium: Online Resource
    Pages: 1 online resource (599 pages)
    Edition: 1st ed.
    ISBN: 9780080858746
    Series Statement: Issn Series ; v.Volume 5
    DDC: 614.432
    Language: English
    Note: Front Cover -- Laboratory Techniques in Biochemistry and Molecular Biology -- Copyright Page -- Contents of parts I and II -- PART I: Techniques of Sample Preparation for Liquid Scintillation Counting -- Contents -- List of abbreviations -- Introduction -- Chapter 1. General principles of liquid scintillation spectrometry -- Chapter 2. Counting systems -- Chapter 3. Preprocessing techniques: general aims and criteria -- Chapter 4. Animal tissue processing -- Chapter 5. Botanical aspects -- Chapter 6. Cell cultures -- Chapter 7. Extracts and chromatographic eluates -- Chapter 8. Macromolecules -- Chapter 9. Electrophoresis, centrifugation and chromatography on solid supports -- Chapter 10. Inorganic applications -- Chapter 11. Quench correction methods, multiple isotope counting and data evaluation -- Chapter 12. Geophysics and archaeology -- Chapter 13. Miscellaneous applications and future prospects -- Appendix I -- Appendix II -- Appendix III -- Appendix IV -- Appendix V -- References -- Subject Index -- PART II: Isoelectric Focusing -- Contents -- Chapter 1. Theory and fundamental aspects of IEF -- Chapter 2. Preparative IEF -- Chapter 3. Analytical IEF -- Chapter 4. General experimental aspects -- Chapter 5. Applications of IEF -- References -- Subject Index.
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  • 2
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 102 (1995), S. 9736-9742 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Exceptionally large pore sizes have recently been reported for polyacrylamide gels grown in the presence of polyethylene glycol. We present small angle static light scattering measurements performed during the evolution of this peculiar gelation process. We give evidence that the large pores are generated by a microsegregation process caused by the competition between gelation and a phase separation of the polymers solution. The separation occurs via spinodal decomposition, and the size of the pores is determined by the stage at which the decomposition is stopped by the gelation process. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 209 (1973), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Planta 136 (1977), S. 115-123 
    ISSN: 1432-2048
    Keywords: Protein ; Storage protein ; Zea ; Zein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The extensive charge heterogeneity of maize (Zea mays L.) zeins observed in isoelectric focusing (IEF) (about 15 bands with pI's in the pH range 6–9) has been found to be independent of extraction procedures or of endosperm development. Zeins do not stain for glycoproteins and exhibit only one lipoprotein component, with pI 3, representing 3–5% of the total protein. Zeins are very resistant to in vitro deamidation, at both acidic and alkaline pH, at high temperatures, and for rather prolonged times. On the basis of the zein content in acidic and basic amino acids, and of the respective pI's exhibited in IEF (mostly in the pH range 7–8) it has been calculated that at least 90% of the glutamic and aspartic acids (≈52 residues out of a total of ≈ 190) are present as asparagine and glutamine. Amino acid analysis of zein fractions isolated by preparative IEF has demonstrated changes in the composition of 18 amino acid residues. However, since these changes affect only neutral and hydrophobic residues, it is concluded that the observed zein heterogeneity is partly based on in vivo deamidation of glutamine and asparagine and partly to spot mutations in some of the genes responsible for zein synthesis.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 324 (1986), S. 262-263 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 333 (1989), S. 728-729 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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