ISSN:
1573-5001
Keywords:
bidirectional coherence transfer
;
Binase
;
gradient coherence selection
;
HNHCACO
;
simultaneous 15N and 13Cα acquisition
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A new triple-resonance pulse sequence, 3D HNHCACO, is introduced and discussed, which identifies sequential correlations of the backbone nuclei (Hα(i-1), Cα(i-1), C′(i-1), NH(i)) of doubly labeled proteins in H2O. The three-dimensional (3D) method utilizes a recording of 15N and 13C resonances in a single indirect time domain, the 13C′ resonance in another indirect time domain, and detects both NH and Hα protons. A bidirectional coherence transfer (NH(i) ↮ N(i) ↮ C′(i-1) ↮ Cα(i-1) ↮ Hα(i-1)) is effectuated, resulting in a single high-resolution 3D spectrum that contains the frequencies of all five backbone nuclei. The experiment was applied to the 12.3 kDa ribonuclease from Bacillus intermedius (Binase).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008229723544
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