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  • 1
    ISSN: 0731-7085
    Keywords: (S)-(+)-4,4'-(1-methyl-1,2-ethandiyl)-bis-(2,6-piperazinedione) ; IR spectroscopy ; X-ray powder diffraction ; binary phase diagram ; differential scanning calorimetry ; enantiomeric purity ; polarimetry.
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2016-06-01
    Description: Motivation: Simple forms of mutualism between microorganisms are widespread in nature. Nevertheless, the role played by the environmental nutrient composition in mediating cross-feeding in microbial ecosystems is still poorly understood. Results: Here, we use mixed-integer bilevel linear programming to investigate the cost of sharing metabolic resources in microbial communities. The algorithm infers an optimal combination of nutrients that can selectively sustain synergistic growth for a pair of species and guarantees minimum cost of cross-fed metabolites. To test model-based predictions, we selected a pair of Escherichia coli single gene knockouts auxotrophic, respectively, for arginine and leucine: argB and leuB and we experimentally verified that model-predicted medium composition significantly favors mutualism. Moreover, mass spectrometry profiling of exchanged metabolites confirmed the predicted cross-fed metabolites, supporting our constraint based modeling approach as a promising tool for engineering microbial consortia. Availability and implementation: The software is freely available as a matlab script in the Supplementary materials. Contact: zampieri@imsb.biol.ethz.ch . Supplementary information: Supplementary data are available at Bioinformatics online.
    Print ISSN: 1367-4803
    Electronic ISSN: 1460-2059
    Topics: Biology , Computer Science , Medicine
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  • 3
    Publication Date: 2012-08-23
    Description: The capacity of microorganisms to respond to variable external conditions requires a coordination of environment-sensing mechanisms and decision-making regulatory circuits. Here, we seek to understand the interplay between these two processes by combining high-throughput measurement of time-dependent mRNA profiles with a novel computational approach that searches for key genetic triggers of transcriptional changes. Our approach helped us understand the regulatory strategies of a respiratorily versatile bacterium with promising bioenergy and bioremediation applications, Shewanella oneidensis , in minimal and rich media. By comparing expression profiles across these two conditions, we unveiled components of the transcriptional program that depend mainly on the growth phase. Conversely, by integrating our time-dependent data with a previously available large compendium of static perturbation responses, we identified transcriptional changes that cannot be explained solely by internal network dynamics, but are rather triggered by specific genes acting as key mediators of an environment-dependent response. These transcriptional triggers include known and novel regulators that respond to carbon, nitrogen and oxygen limitation. Our analysis suggests a sequence of physiological responses, including a coupling between nitrogen depletion and glycogen storage, partially recapitulated through dynamic flux balance analysis, and experimentally confirmed by metabolite measurements. Our approach is broadly applicable to other systems.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
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  • 4
    Publication Date: 2016-10-06
    Description: Heavy precipitation is a major hazard over Europe. It is well established that climate model projections indicate a tendency towards more extreme daily rainfall events. It is still uncertain, however, how this changing intensity translates at the sub-daily time scales. The main goal of the present study is to examine possible differences in projected changes in intense precipitation events over Europe at the daily and sub-daily (3-hourly) time scales using a state-of-the-science climate model. The focus will be on one Representative Concentration Pathway (RCP 8.5), considered as illustrative of a high rate of increase in greenhouse gas concentrations over this century. There are statistically significant differences in intense precipitation projections (up to 40%) when comparing the results at the daily and sub-daily time scales. Over north-eastern Europe, projected precipitation intensification at the 3-hour scale is lower than at the daily scale. On the other hand, Spain and the western seaboard exhibit an opposite behaviour, with stronger intensification at the 3-hour scale rather than daily scale. While the mean properties of the precipitation distributions are independent of the analysed frequency, projected precipitation intensification exhibits regional differences. This finding has implications on the extrapolation of impacts of intense precipitation events, given the daily time scale the analyses are usually performed at.
    Description: Published
    Description: 6193–6203
    Description: 4A. Clima e Oceani
    Description: JCR Journal
    Description: restricted
    Keywords: rainfall ; extreme events ; heavy precipitation ; snow ; europe ; 01. Atmosphere::01.01. Atmosphere::01.01.02. Climate
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 5
    Publication Date: 2017-04-04
    Description: Precipitation extremes are expected to increase in a warming climate, thus it is essential to characterise their potential future changes. Here we evalu- ate eight high-resolution Global Climate Model simulations in the twenti- eth century and provide new evidence on projected global precipitation ex- tremes for the 21st century. A significant intensification of daily extremes for all seasons is projected for the mid and high latitudes of both hemispheres at the end of the present century. For the subtropics and tropics, the lack of reliable and consistent estimations found for both the historical and fu- ture simulations might be connected with model deficiencies in the repre- sentation of organised convective systems. Low inter-model variability and good agreement with high-resolution regional observations are found for the twentieth century winter over the Northern Hemisphere mid and high lat- itudes.
    Description: Published
    Description: 4887–4892
    Description: 3.7. Dinamica del clima e dell'oceano
    Description: JCR Journal
    Description: open
    Keywords: extreme events ; precipitation ; cmip5 ; 01. Atmosphere::01.01. Atmosphere::01.01.02. Climate
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 6
    Publication Date: 2017-04-04
    Description: In this study, we analyse the observed long-term discharge time-series of the Rhine, the Danube, the Rhone and the Po rivers. These rivers are characterised by different seasonal cycles reflecting the diverse climates and morphologies of the Alpine basins. However, despite the intensive and varied water management adopted in the four basins, we found common features in the trend and low-frequency variability of the spring discharge timings. All the discharge time-series display a tendency towards earlier spring peaks of more than two weeks per century. These results can be explained in terms of snowmelt, total precipitation (i.e. the sum of snowfall and rainfall) and rainfall variability. The relative importance of these factors might be different in each basin. However, we show that the change of seasonality of total precipitation plays a major role in the earlier spring runoff over most of the Alps.
    Description: Published
    Description: 222-232
    Description: 4A. Clima e Oceani
    Description: JCR Journal
    Description: open
    Keywords: rivers ; alps ; precipitation ; 01. Atmosphere::01.01. Atmosphere::01.01.02. Climate
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 7
    Publication Date: 2017-04-04
    Description: Global warming is believed to be responsible for the reduction of snow amount and duration over the Alps. In fact, a rapid shortening of the snowy season has been measured and perceived by ecosystems and society in the past 30 years, despite the large year-to-year variability. This trend is projected to continue during the 21st century in the climate change scenarios with increasing greenhouse gas concentrations. Superimposed on the long-term trend, however, there is a low-frequency variability of snowfall associated with multi-decadal changes in the large-scale circulation. The amplitude of this natural low-frequency variation might be relatively large, determining rapid and substantial changes of snowfall, as recently observed. This is already known for winter snowfall over the Alps in connection with the recent tendency toward the positive phase of the North Atlantic Oscillation. In this study, we show that the low-frequency variability of Alpine spring snowfall in the past 150 years is affected by the Atlantic Multi-decadal Oscillation (AMO), which is a natural periodic fluctuation of Northern Atlantic sea surface temperature. Therefore, the recently observed spring snowfall reduction might be, at least in part, explained by the shift toward a positive AMO phase that happened in the 1990s.
    Description: Published
    Description: 034026 (7pp)
    Description: 3.7. Dinamica del clima e dell'oceano
    Description: JCR Journal
    Description: restricted
    Keywords: AMO ; snow ; alps ; 01. Atmosphere::01.01. Atmosphere::01.01.02. Climate
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 8
    Publication Date: 2017-04-04
    Description: Climate model simulations are currently the main tool to provide information about possible future climates. Apart from scenario uncertainties and model error, internal variability is a major source of uncertainty, complicating predictions of future changes. Here, a suite of statistical tests is proposed to determine the shortest time window necessary to capture the internal precipitation variability in a stationary climate. The length of this shortest window thus expresses internal variability in terms of years. The method is applied globally to daily precipitation in a 200-yr preindustrial climate simulation with the CMCC-CM coupled general circulation model. The two-sample Cramér–von Mises test is used to assess differences in precipitation distribution, the Walker test accounts for multiple testing at grid cell level, and field significance is determined by calculating the Bejamini–Hochberg false-discovery rate. Results for the investigated simulation show that internal variability of daily precipitation is regionally and seasonally dependent and that regions requiring long time windows do not necessarily coincide with areas with large standard deviation. The estimated time scales are longer over sea than over land, in the tropics than in midlatitudes, and in the transitional seasons than in winter and summer. For many land grid cells, 30 seasons suffice to capture the internal variability of daily precipitation. There exist regions, however, where even 50 years do not suffice to sample the internal variability. The results show that diagnosing daily precipitation change at different times based on fixed global snapshots of one climate simulation might not be a robust detection method.
    Description: Published
    Description: 3624–3630
    Description: 4A. Clima e Oceani
    Description: JCR Journal
    Description: restricted
    Keywords: precipitation ; internal variability ; 01. Atmosphere::01.01. Atmosphere::01.01.02. Climate
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 9
    Publication Date: 2017-04-04
    Description: In this work the authors investigate possible changes in the distribution of heavy precipitation events under a warmer climate, using the results of a set of 20 climate models taking part in the Coupled Model Intercomparison Project phase 5 effort (CMIP5). Future changes are evaluated as the difference between the last four decades of the 21st and the 20th Century assuming the Representative Concentration Pathway RCP8.5 scenario. As a measure of the width of the right tail of the precipitation distribution, we use the difference between the 99th and the 90th percentiles. Despite a slight tendency to underestimate the observed heavy precipitation, the considered CMIP5 models well represent the observed patterns in terms of the ensemble average, during both summer and winter seasons for the 1997-2005 period. Future changes in average precipitation are consistentwith previous findings based on CMIP3 models. CMIP5 models show a projected increase for the end of the twenty-first century of the width of the right tail of the precipitation distribution, particularly pronounced over India, South East Asia, Indonesia and Central Africa during borealsummer, as well as over South America and southern Africa during boreal winter.
    Description: Published
    Description: 7902–7911
    Description: 3.7. Dinamica del clima e dell'oceano
    Description: JCR Journal
    Description: open
    Keywords: precipitation ; extreme events ; climate change ; 01. Atmosphere::01.01. Atmosphere::01.01.02. Climate
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 10
    Publication Date: 2018-05-02
    Description: We analyze the influence of the Atlantic sea surface temperature multi-decadal variability on the day-by-day sequence of large-scale atmospheric circulation patterns (i.e. the “weather regimes”) over the Euro-Atlantic region. In particular, we examine of occurrence of weather regimes from 1871 to present. This analysis is conducted by applying a clustering technique on the daily mean sea level pressure field provided by the 20th Century Reanalysis project, which was successfully applied in other studies focused on the Atlantic Multi-decadal Oscillation (AMO). In spring and summer, results show significant changes in the frequencies of certain weather regimes associated with the phase shifts of the AMO. These changes are consistent with the seasonal surface pressure, precipitation, and temperature anomalies associated with the AMO shifts in Europe.
    Description: Published
    Description: 92-100
    Description: 4A. Oceanografia e clima
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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