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  • 1
    Electronic Resource
    Electronic Resource
    Adenylate cyclase induces intracellular calcium increase in single human epidermal keratinocytes measured by fluorescence microscopy using Fura 2-AM (1992)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 127 (1992), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Intracellular calcium ([Ca2+]i) is an important second messenger of extracellular signals to induce various cellular responses. Extracellular and intracellular Ca2+ are considered to be important for cellular differentiation and proliferation of epidermal keratinocytes. Several mechanisms which increase [Ca2+]i have been demonstrated in various tissues, but in epidermal keratinocytes these mechanisms are poorly understood. In epidermal keratinocytes the adenylate cyclase-cyclic AMP response is thought to regulate cell proliferation and differentiation. However, the series of reactions which follow the cyclic AMP response remain unknown. Beta-adrenergic agonists increase [Ca2+]i in cultured epidermal keratinocytes, and we have therefore studied whether stimulation of keratinocyte adenylate cyclase could induce [Ca2+]i increase, by using fluorescence microscopy with Fura 2-AM.Adenosine and histamine, which are known to be keratinocyte adenylate cyclase receptor agonists, induced transient [Ca2+]i increase, as did epinephrine. In addition, forskolin, a direct adenylate cyclase activator, and dibutylyl-cyclic AMP also induced an increase in [Ca2+]i. In a calcium-free medium epinephrine, adenosine, histamine and dibutylyl-cyclic AMP induced an increase in [Ca2+]i. These results suggest that cyclic AMP in human epidermal keratinocytes regulates [Ca2+]i, which is released from intracellular stores.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1992.tb14871.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Subcutaneous nodules on the buttocks as a manifestation of dialysis-related amyloidosis: a clinicopathological entity? (2003)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 149 (2003), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary  While β2-microglobulin amyloidosis occurring in patients undergoing long-term dialysis is frequently associated with joint involvement, skin lesions have rarely been encountered. We report a 57-year-old man with extensive subcutaneous amyloid deposition forming large nodules on the buttocks; the patient had been on maintenance dialysis for 28 years. Although this condition is rare, a review of the literature indicates that the majority of such lesions occur around the buttock region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2003.05466.x
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  • 3
    Electronic Resource
    Electronic Resource
    Stimulation of prostaglandin E adenylate cyclase response in pig epidermis by hydrocortisone (1989)
    Springer
    Archives of dermatological research 281 (1989), S. 215-217 
    Details
    ISSN: 1432-069X
    Keywords: Prostaglandin E ; Adenylate cyclase ; Cyclic AMP ; Psoriasis ; Epidermis ; Hydrocortisone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00456398
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  • 4
    Electronic Resource
    Electronic Resource
    Adenylate cyclase induces intracellular Ca2+ increase in single human epidermal keratinocytes of the epidermal sheet as measured by digital imaging microscopy using Fura 2-AM (1991)
    Springer
    Archives of dermatological research 283 (1991), S. 91-95 
    Details
    ISSN: 1432-069X
    Keywords: Intracellular free calcium ; Human epidermis ; Beta-adrenergic receptor ; Adenylate cyclase ; Fura 2 ; Digital imaging microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intracellular Ca2+ ([Ca2+]i) is thought to act as a second messenger of transmembrane signalling systems. However, no measurement of [Ca2+]i has been made in intact epidermal keratinocytes. We have developed a method for measuring [Ca2+]i in human keratinocytes from pure epidermal sheet by the application of digital imaging fluorescence microscopy with the use of Fura 2-AM. Normal human pure epidermal sheets were obtained by dispase treatment. Epinephrine and salbutamol induced transient [Ca2+]i increases. Propranolol, a Β-antagonist, inhibited this response, while prazosin and yohimbine (alpha1- and alpha2-antagonists, respectively) did not affect the response. Histamine and adenosine, also receptor agonists of the epidermal adenylate cyclase system, induced a similar [Ca2+]i increase, as did forskolin, a direct activator of adenylate cyclase. These data coincide with those previously presented for cultured human epidermal keratinocytes, and reveal that adenylate cyclase activation induces an increase of [Ca2+]i in intact epidermal cells. This technique enables the kinetics of [Ca2+]i in various skin disorders to be investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00371615
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  • 5
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    Rapid evolution of the innermost dust disc of protoplanetary discs surrounding intermediate-mass stars (2014)
    Oxford University Press
    Details
    Publication Date: 2014-06-26
    Description: We derived the intermediate-mass (~=1.5–7 M ) disc fraction (IMDF) in the near-infrared JHK photometric bands as well as in the mid-infrared (MIR) bands for young clusters in the age range of 0 to ~10 Myr. From the JHK IMDF, the lifetime of the innermost dust disc (~0.3 au; hereafter the K disc) is estimated to be ~3 Myr, suggesting a stellar mass ( M * ) dependence of K -disc lifetime (~=1.5-7 M). However, from the MIR IMDF, the lifetime of the inner disc (~5 au; hereafter the MIR disc) is estimated to be ~6.5 Myr, suggesting a very weak stellar mass dependence ( M * ). The much shorter K -disc lifetime compared to the MIR-disc lifetime for intermediate-mass (IM) stars suggests that IM stars with transition discs , which have only MIR excess emission but no K -band excess emission, are more common than classical Herbig Ae/Be stars, which exhibit both. We suggest that this prominent early disappearance of the K disc for IM stars is due to dust settling/growth in the protoplanetary disc, and it could be one of the major reasons for the paucity of close-in planets around IM stars.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
    Published by Oxford University Press
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