GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Gene Biotechnology. (2011)
    Baton Rouge :Taylor & Francis Group,
    Details
    Keywords: Genetic engineering -- Laboratory manuals. ; Molecular biology -- Laboratory manuals. ; Electronic books.
    Description / Table of Contents: Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book: Discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies Contains clearly detailed, step-by-step protocols with helpful troubleshooting tips Addresses the needs of researchers in academic and commercial environments Guides graduate students in designing, implementing, and evaluating experimental projects. Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation. Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source for the most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research.
    Type of Medium: Online Resource
    Pages: 1 online resource (562 pages)
    Edition: 3rd ed.
    ISBN: 9781439848326
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=726865
    DDC: 572.8607
    Language: English
    Note: Front Cover -- Contents -- Preface -- Authors -- Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding -- Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR -- Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries -- Chapter 4: Subcloning of Genes or DNA Fragments -- Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing -- Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins -- Chapter 7: Characterization of DNA or Genes by Southern Blot Hybridization -- Chapter 8: Gene Overexpression by Sense RNA in Mammalian Systems -- Chapter 9: Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies -- Chapter 10: Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR -- Chapter 11: Analysis of Gene Expression at Proteomic Level via Western Blotting -- Chapter 12: Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization -- Chapter 13: Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization -- Chapter 14: In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs -- Chapter 15: Isolation and Characterization of Genes from Genomic DNA Libraries -- Chapter 16: Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression -- Chapter 17: Strategies for Gene Double Knockout -- Chapter 18: Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells -- Chapter 19: Quantitative Analysis of Functional Genome by Real-Time RT-PCR -- Chapter 20: High-Throughput Analysis of Gene Expression by Cutting-Edge Technology-DNA Microarrays (Gene Chips) -- Chapter 21: Construction and Screening of Human Antibody Libraries Using Phage Display Technology. , Chapter 22: Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology -- Chapter 23: Strategies for Gene Cloning, Expression, and Identification of Protein-Protein Interaction -- Chapter 24: Conditional Gene Knockout -- Chapter 25: How to Write a Research Manuscript for Publication in an English Journal -- Chapter 26: How to Protect Your Discovery and Invention : Patent 101 -- Chapter 27: Determination of Transgene Copy Numbers and Practical Biocalculation -- Back Cover.
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    GEOMAR EBL
  • 2
    Electronic Resource
    Electronic Resource
    Infrared remote sensing and determination of pollutants in gas plumes (1973)
    s.l. : American Chemical Society
    Environmental science & technology 7 (1973), S. 417-423 
    Details
    ISSN: 1520-5851
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/es60077a007
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    α-2 macroglobulin gene and Alzheimer disease (1999)
    [s.l.] : Nature America Inc.
    Nature genetics 22 (1999), S. 17-19 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Blacker et al. reported an association between a deletion in exon 18 of the α-2 macroglobulin (A2M) gene and Alzheimer disease (AD) in a sample of affected and unaffected siblings from families segregating AD. They observed that the degree of conferred risk for AD in A2M allele 2 (A2M*2) ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/8726
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The extraction of organotin compounds from polyvinyl chloride pipe (1989)
    Springer
    Archives of environmental contamination and toxicology 18 (1989), S. 839-843 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract The extraction (leaching) of organotin stabilizers from commercial polyvinyl chloride pipe into flowing ultra-high purity water was examined as a function of time and flow rate. A recirculation system which consisted of 46 meters of pipe was used in the dynamic tests. The extraction rates for 1.9 cm diameter pipe from two different manufacturers followed a decaying exponential function and decreased significantly during a period of four hr of water recirculation. Dissolved organotin concentration reached 95% of its steady state value within about 12 hr. The extraction resulted from organotin at or near the inner wall of the pipe. Diffusion of organotin from the bulk of the pipe wall to the inner wall was apparently negligible. Mass transfer coefficients for the extraction process were correlated with Reynolds number.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01160298
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Synthesis and characterization of amphipathic poly(methyl methacrylate)-b-poly(ethylene oxide) diblock copolymers (1993)
    Springer
    Polymer bulletin 31 (1993), S. 531-538 
    Details
    ISSN: 1436-2449
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Summary This paper describes the synthesis and characterization of amphipathic diblock copolymers of poly(methyl methacrylate) (PMMA) and poly(ethylene oxide) (PEO). The synthesis involved the coupling of acyl chloride-terminated PMMA block with methoxy poly(ethylene oxide) (MPEO). Carboxylic acid chloride-terminated PMMA was generated by treating with thionyl chloride the parent carboxylic PMMA, which was prepared by free radical polymerization of methyl methacrylate (MMA) using benzoyl peroxide (BPO) as the initiator and β-mercaptopropionic acid (MPA) as the chain transfer agent. The proposed mechanism of MMA polymerization is in good agreement with the experimental results which indicate that as a side reaction nonfunctional (aromatic) counterpart is produced in a small quantity. The coupling of the acyl chloride-terminated PMMA with MPEO was quantitative.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00297888
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    facet.materialart.
    Unknown
    Viral aetiology of acute respiratory infections among children and associated meteorological factors in southern China (2015)
    BioMed Central
    Details
    Publication Date: 2015-03-14
    Description: Background: Acute respiratory infections (ARIs) are common in children and mostly caused by viruses, but the significance of the detection of multiple viruses in ARIs is unclear. This study investigated 14 respiratory viruses in ARIs among children and associated meteorological factors in Shantou, southern China. Methods: Paired nasal/throat-flocked swabs collected from 1,074 children with ARIs, who visited outpatient walk-in clinics in a tertiary hospital between December 2010 and November 2011, were examined for fourteen respiratory viruses - influenza viruses (FluA, FluB), respiratory syncytial viruses (RSV A and B), human coronaviruses (hCoV: 229E, OC43, HKU1, NL63), human metapneumoviruses (hMPV A and B), parainfluenza viruses (PIV1-4), human rhinoviruses (HRV A, B, C), enteroviruses (EV), adenoviruses (ADV), human bocavirus (hBoV), and human parechoviruses (hPeV) - by multiplex real-time PCR. Results: We identified at least one virus in 82.3% (884/1,074) and multiple viruses in 38.6% (415/1,074) of patients. EV and HRV were the most frequently detected single viruses (42.3%, 374/884 and 39.9%, 353/884 respectively) and co-detected pair (23.1%, 96/415). Overlapping seasonal trends of viruses were recorded over the year, with dual peaks for EV and single peaks for the others. By logistic regression analysis, EV was positively associated with the average temperature and humidity, hCoV, and PIV4, but negatively with HRV, PIV3, and hBoV. HRV was inversely associated with EV and PIV3. Conclusions: This study reports high viral detection and co-detection rates in pediatric ARI cases mainly due to EV and HRV. Many viruses circulated throughout the year with similar seasonal trends in association with temperature, humidity, and wind velocity. Statistically significant associations were present among the viruses. Understanding the polyviral etiology and viral interactions in the cases with multiple viruses warrants further studies.
    Electronic ISSN: 1471-2334
    Topics: Medicine
    Published by BioMed Central
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 7
    facet.materialart.
    Unknown
    Reprocessing unsatisfactory ThinPrep Papanicolaou tests using a modified SurePath preparation technique (2014)
    Wiley-Blackwell
    Details
    Publication Date: 2014-02-21
    Description: BACKGROUND The frequency of unsatisfactory gynecologic specimens has increased in the study laboratory over the last few years due to the advent of personal lubricants. Similarly, lysed blood, protein, and necrotic debris present a challenge in terms of negative cell transference caused by a clogged filter. In the current study, the authors evaluated the potential use of a modified SurePath reprocessing technique to decrease the frequency of unsatisfactory specimens. METHODS An aliquot for human papillomavirus testing was set aside and the remaining specimen in the ThinPrep vial was submitted for sedimentation. A methanol wash was performed using preservative that was added to the remaining sediment. The specimen was vortexed and a protein wash of Tris-buffered deionized water was added before processing. The specimens were stained using the ThinPrep staining protocol. Both the original specimen and the reprocessed specimens were manually reviewed by 1 cytotechnologist and 2 pathologists to determine specimen adequacy. RESULTS A total of 1937 ThinPrep Papanicolaou tests were reprocessed and examined. Of these, 1093 (56%) specimens were satisfactory, 624 of which (57%) demonstrated evidence of a transformation zone component. Epithelial cell abnormalities were identified in 116 specimens (10.6%), including 11 high-grade squamous intraepithelial lesion specimens (1.0%); 5 specimens with a classification of atypical squamous cells, cannot rule out a high-grade lesion (0.5%); 21 low-grade squamous intraepithelial lesion specimens (1.9%); and 79 specimens classified as atypical squamous cells of undetermined significance (7.2%). CONCLUSIONS The modified SurePath processing technique was adept at handling nearly all of the challenges that biological and environmental conditions (blood, protein, lubricant, etc) present in liquid-based filter preparations. A total of 1093 (56%) of 1937 unsatisfactory ThinPrep Papanicolaou tests were converted to a satisfactory state, resulting in 116 abnormalities (10.6%) being diagnosed that otherwise would have gone undetected. Cancer (Cancer Cytopathol) 2014. © 2014 American Cancer Society.
    Topics: Medicine
    Published by Wiley-Blackwell on behalf of The American Cancer Society.
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 8
    facet.materialart.
    Unknown
    Crystal structure of an amphiphilic foldamer reveals a 48-mer assembly comprising a hollow truncated octahedron (2014)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2014-04-09
    Description: Article Foldamers are small molecules or oligomers that can adopt secondary and tertiary structures due to noncovalent interactions. Here, the authors show that an amphiphilic foldamer can adopt a hollow truncated octahedron crystal structure comprising of 48 individual foldamer units. Nature Communications doi: 10.1038/ncomms4581 Authors: Vincenzo Pavone, Shao-Qing Zhang, Antonello Merlino, Angela Lombardi, Yibing Wu, William F. DeGrado
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
    Published by Nature Publishing Group (NPG)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 9
    facet.materialart.
    Unknown
    Discovery of the 3-Imino-1,2,4-thiadiazinane 1,1-Dioxide Derivative Verubecestat (MK-8931)–A β-Site Amyloid Precursor Protein Cleaving Enzyme 1 Inhibitor for the Treatment of Alzheimer’s Disease (2016)
    American Chemical Society (ACS)
    Details
    Publication Date: 2016-11-19
    Description: Journal of Medicinal Chemistry DOI: 10.1021/acs.jmedchem.6b00307
    Topics: Chemistry and Pharmacology
    Published by American Chemical Society (ACS)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 10
    facet.materialart.
    Unknown
    Cardiac Regeneration [Congenital Heart Disease Compendium] (2017)
    American Heart Association (AHA)
    Details
    Publication Date: 2017-03-17
    Description: Palliative surgery for congenital heart disease has allowed patients with previously lethal heart malformations to survive and, in most cases, to thrive. However, these procedures often place pressure and volume loads on the heart, and over time, these chronic loads can cause heart failure. Current therapeutic options for initial surgery and chronic heart failure that results from failed palliation are limited, in part, by the mammalian heart’s low inherent capacity to form new cardiomyocytes. Surmounting the heart regeneration barrier would transform the treatment of congenital, as well as acquired, heart disease and likewise would enable development of personalized, in vitro cardiac disease models. Although these remain distant goals, studies of heart development are illuminating the path forward and suggest unique opportunities for heart regeneration, particularly in fetal and neonatal periods. Here, we review major lessons from heart development that inform current and future studies directed at enhancing cardiac regeneration.
    Keywords: Congenital Heart Disease, Treatment
    Print ISSN: 0009-7330
    Electronic ISSN: 1524-4571
    Topics: Medicine
    Published by American Heart Association (AHA)
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...