Description: Author Posting. © Macmillan Publishers, 2009. This is the author's version of the work. It is posted here by permission of Macmillan Publishers for personal use, not for redistribution. The definitive version was published in Nature Geoscience 2 (2009): 67-72, doi:10.1038/ngeo382.

Description: The process of open-ocean convection in the subpolar North Atlantic Ocean forms a dense water mass that impacts the meridional overturning circulation and heat flux, and sequesters atmospheric carbon. In recent years the convection has been shallow or nonexistent, which could be construed as a consequence of a warmer climate. However, in the winter of 2007-08 deep convection returned to the subpolar gyre in both the Labrador and Irminger Seas. Here we document this return and elucidate the reasons why it happened. Profiling float data from the Argo programme are used to document the deep mixing, and a variety of in-situ, satellite, and reanalysis products are analyzed to describe the conditions leading to the overturning. The transition to a convective state took place abruptly, without going through a preconditioning phase, which is contrary to general expectations. Changes in the hemispheric air temperature, tracks of storms, flux of freshwater to the Labrador Sea, and distribution of pack ice all conspired to enhance the air-sea heat flux, resulting in the deep overturning. This study illuminates the complexity of the North Atlantic convective system.

Description: Support for this work was provided by the Ocean Sciences Division of the National Science Foundation.

Description: Background: The clinical dehydration scale (CDS) is a quick, easy-to-use tool with 4 clinical items and a score of 1-8 that serves to classify dehydration in children with gastroenteritis as no, some or moderate/severe dehydration. Studies validating the CDS (Friedman JN) with a comparison group remain elusive. We hypothesized that the CDS correlates with a wide spectrum of established markers of dehydration, making it an appropriate and easy-to-use clinical tool. Methods: This study was designed as a prospective double-cohort trial in a single tertiary care center. Children with diarrhea and vomiting, who clinically required intravenous fluids for rehydration, were compared with minor trauma patients who required intravenous needling for conscious sedation. We compared the CDS with clinical and urinary markers (urinary electrolytes, proteins, ratios and fractional excretions) for dehydration in both groups using receiver operating characteristic (ROC) curves to determine the area under the curve (AUC). Results: We enrolled 73 children (male = 36) in the dehydration group and 143 (male = 105) in the comparison group. Median age was 32 months (range 3-214) in the dehydration and 96 months (range 2.6-214 months, p 〈 0.0001) in the trauma group. Median CDS was 3 (range 0-8) within the dehydration group and 0 in the comparison group (p 〈 0.0001). The following parameters were statistically significant (p 〈 0.05) between the comparison group and the dehydrated group: difference in heart rate, diastolic blood pressure, urine sodium/potassium ratio, urine sodium, fractional sodium excretion, serum bicarbonate, and creatinine measurements. The best markers for dehydration were urine Na and serum bicarbonate (ROC AUC = 0.798 and 0.821, respectively). CDS was most closely correlated with serum bicarbonate (Pearson r = -0.3696, p = 0.002). Conclusion: Although serum bicarbonate is not the gold standard for dehydration, this study provides further evidence for the usefulness of the CDS as a dehydration marker in children.Trial registration: Registered at ClinicalTrials.gov (NCT00462527) on April 18, 2007.

Description: Until recently, retention of introns in mature mRNAs has been regarded as a consequence of mis-splicing. Intron-retaining transcripts are thought to be non-functional because they are readily degraded by nonsense-mediated decay. However, recent advances in next-generation sequencing technologies have enabled the detection of numerous transcripts that retain introns. As we review herein, intron-retaining mRNAs play an essential conserved role in normal physiology and an emergent role in diverse diseases. Intron retention should no longer be overlooked as a key mechanism that independently reduces gene expression in normal biology. Exploring its contribution to the development and/or maintenance of diseases is of increasing importance. Intron retention was once thought to be a consequence of mis-splicing but it is now established as an important mechanism controlling gene expression within the cell. Here, we review known functions of intron-retaining mRNAs in normal physiology, their role in disease, and explore potential novel cell functions for intron-retaining mRNAs.