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  • 1
    Electronic Resource
    Electronic Resource
    Two-dimensional polyacrylamide gel electrophoresis of water-soluble erythrocyte membrane proteins (1981)
    Weinheim : Wiley-Blackwell
    Electrophoresis 2 (1981), S. 240-246 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A method is reported for analyzing the oligomeric state of proteins in a mixture by multidimensional electrophoresis. Water-soluble proteins prepared from human erythrocyte membranes were separated by means of gel filtration followed by electrophoresis in detergent-free disc gels. The major bands in detergent-free disc gels were identified by means of electrophoresis into a second-dimensional slab gel containing sodium dodecyl sulphate. Detergent-free disc gels were also subjected to two-dimensional electrophoresis into detergent-free slab gels consisting of a continuous gradient of polyacrylamide gel. It was shown that many of the water-soluble proteins existed as distinct oligomers. Spectrin aggregates (tetramer and dimmer) accounted for the slowest moving bands in the detergent-free disc gels. A water-soluble protein of the component 3 region appeared to be present as a hexamer, while component 4.1 was present as a tetramer. Components 4.3, 4.9 and 5 appeared to be present in a large number of aggregated states. Components 7 and 8 formed a heteropolymeric protein complex.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150020408
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    Paper (German National Licenses)
    Fulltext
  • 2
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    Evidence for low genetic divergence among Galápagos Opuntia cactus species (2021)
    In:  http://aquaticcommons.org/id/eprint/8580 | 704 | 2012-04-16 19:53:01 | 8580 | Fundacion Charles Darwin Foundation
    Details
    Publication Date: 2021-06-24
    Description: U.S. National Science Foundation
    Description: Wake Forest University
    Description: Coca-Cola Foundation
    Keywords: Biology ; Conservation ; Opuntia ; cactus ; genetics ; evolution ; divergence ; morphology ; Galápagos ; Puerto Lopez ; Ecuador ; Isla Monito ; Puerto Rico
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 11-15
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    PAPER (OA)
  • 3
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    Experimental and numerical investigation of hydrate-guest molecule exchange kinetics (2016)
    Elsevier
    In:  Journal of Natural Gas Science and Engineering, 35 . pp. 1480-1489.
    Details
    Publication Date: 2020-09-18
    Description: The 2012 Ignik Sikumi #1 Field Trial in Alaska was designed to test at field scale a methane hydrate production methodology that involves injecting carbon dioxide (CO2) in situ to exchange with methane (CH4) within a hydrate structure, thus releasing the methane for production. Since the completion of the field trial, experimental and numerical investigations have sought to better understand observations in terms of CH4 production and changes to the hydrate bearing formation. Collectively, these insights may lead to more effective strategies for producing CH4 from naturally occurring hydrates. This study presents results from a laboratory experiment performed at conditions experienced during the field trial (8.27 MPa and 275.15 K) to collect data relating to the CH4 molecular exchange process. The experiment intentionally isolated the kinetic guest molecule exchange process from additional hydrate formation and mechanical changes to the hydrate bearing sand. Data were used to inform development of numerical models that were then used to fit key hydrate parameters (bound water saturation, kinetic formation constant, kinetic exchange constant, and preferential exchange weighting factors) and analyze experimental results. Results were in agreement with observations of preferential CO2/CH4 guest molecule exchange at Ignik Sikumi #1 and from previous experimental studies while providing quantitative estimates of changing hydrate compositions. Additionally, simulations confirmed hydrate behavior and composition profiles otherwise indirectly evidenced by experimental results.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1016/j.jngse.2016.03.080
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 4
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    Quantification of CO2 Mineralization at the Wallula Basalt Pilot Project (2020)
    ACS (American Chemical Society)
    Details
    Publication Date: 2023-02-08
    Description: In 2013, the Pacific Northwest National Laboratory led a geologic carbon sequestration field demonstration where ∼1000 tonnes of CO2 was injected into several deep Columbia River Basalt zones near Wallula, Washington. Rock core samples extracted from the injection zone two years after CO2 injection revealed nascent carbonate mineralization that was qualitatively consistent with expectations from laboratory experiments and reactive transport modeling. Here, we report on a new detailed analysis of the 2012 pre-injection and 2015 post-injection hydrologic tests that capitalizes on the difference in fluid properties between scCO2 and water to assess changes in near-field, wellbore, and reservoir conditions that are apparent approximately two years following the end of injection. This comparative hydrologic test analysis method provides a new way to quantify the amount of injected CO2 that was mineralized in the field test. Modeling results indicate that approximately 60% of the injected CO2 was sequestered via mineralization within two years, with the resulting carbonates occupying ∼4% of the available reservoir pore space. The method presented here provides a new monitoring tool to assess the fate of CO2 injected into chemically reactive basalt formations but could also be adapted for long-term monitoring and verification within more traditional subsurface carbon storage reservoirs.
    Type: Article , PeerReviewed
    Format: text
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    OceanRep: Article in a Scientific Journal - peer-reviewed
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