Keywords:
Sea squirts.
;
Electronic books.
Type of Medium:
Online Resource
Pages:
1 online resource (909 pages)
Edition:
1st ed.
ISBN:
9780080496597
Series Statement:
Issn Series
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=296979
Language:
English
Note:
Front Cover -- Methods in Cell Biology -- Copyright Page -- Contents -- Contributors -- Preface -- Dedication -- Chapter 1. The Invertebrate Deuterostomes: An Introduction to Their Phylogeny, Reproduction, Development, and Genomics -- I. Introduction -- II. Phylogeny -- III. Reproduction -- IV. Development -- V. Genomics -- References -- Part I: Procurement, Maintenance & -- Culture of Oocytes, Embryos, Larvae and Adults -- Chapter 2. Care and Maintenance of Adult Echinoderms -- I. Overview -- II. Introduction -- III. Adult Echinoderm Models: Their Reproductive Cycles and Gametogenesis -- IV. Obtaining Adult Echinoderms -- V. Maintenance of Adult Echinoderms in Land-Based Systems -- VI. Care and Handling of Adult Echinoderms -- References -- Chapter 3. Echinoderm Eggs and Embryos: Procurement and Culture -- I. Introduction -- II. Method for Sea Urchins (Class Echinoidea) -- III. Method for Sea Stars (Class Asteroidea) -- IV. Method for Brittle Stars (Class Ophiuroidea, Order Ophiurida) -- V. Method for Sea Cucumbers (Class Holothuroidea) -- VI. Method for Interspecific Crosses -- VII. Summary -- References -- Chapter 4. Culture of Echinoderm Larvae through Metamorphosis -- I. Introduction -- II. Materials for Culturing Algae and Larvae -- III. Establishing Algal Cultures -- IV. Culturing Echinoderm Larvae -- V. Metamorphosis and Beyond -- References -- Chapter 5. Obtaining and Handling Echinoderm Oocytes -- I. Introduction -- II. Experimental Preparation -- III. Methods of Oocyte Collection -- IV. Sea Urchin Oocytes Cultured In Vitro -- V. Labeling of Oocyte Components -- VI. Introduction of Experimental Substances into the Oocytes -- VII. Concluding Remarks/Outlook -- References -- Chapter 6. Procurement and Culture of Ascidian Embryos -- I. Overview -- II. Ascidian Development and Metamorphosis -- III. Larval Tissue Specification.
,
IV. Experimental Techniques -- V. Protocols -- References -- Chapter 7. Culture of Adult Ascidians and Ascidian Genetics -- I. Overview -- II. Introduction -- III. Culturing Ascidians -- IV. Induced Developmental Mutants and Natural Variants/Mutants -- V. Linkage Analysis and Mapping Genes in Ascidians -- References -- Chapter 8. Hemichordate Embryos: Procurement, Culture, and Basic Methods -- I. Introduction -- II. Procurement, Spawning, and Culture of S. kowalevskii -- III. Procurement, Spawning, and Culture of Ptychodera flava -- IV. Removal of Vitelline Envelope in S. kowalevskii -- V. Whole-Mount In Situ Hybridization -- VI. Preparation of Blocking Reagent for P. flava -- VII. Materials and Reagents -- References -- Chapter 9. Cephalochordate (Amphioxus) Embryos: Procurement, Culture, and Basic Methods -- I. Overview -- II. Introduction -- III. Obtaining Gametes of Branchiostoma floridae -- IV. Raising Embryos -- V. Manipulating Embryos -- VI. Amphioxus Resources Available -- VII. Concluding Remarks -- References -- Part II: Embryological Approaches -- Chapter 10. Quantitative Microinjection of Oocytes, Eggs, and Embryos -- I. Introduction -- II. Methods -- III. Equipment and Supplies (Prices are as of December, 2002) -- References -- Chapter 11. Blastomere Isolation and Transplantation -- I. Introduction -- II. Preparation of Mouth Pipettes and Needles -- III. Removal of Fertilization Envelope of Sea Urchin Eggs -- IV. Isolation and Recombination of Blastomeres in Sea Urchin Embryos -- V. Blastomere Isolation and Transplantation in Starfish Embryos -- VI. Blastomere Isolation and Transplantation in Amphioxus -- VII. Blastomere Isolation and Transplantation in Ascidians -- VIII. Lineage Tracing, Blastomere Isolation, and Transplantation in Hemichordates -- References -- Chapter 12. Isolation and Culture of Micromeres and Primary Mesenchyme Cells.
,
I. Introduction -- II. Isolation and Culture of Micromeres -- III. Isolation and Culture of Primary Mesenchyme Cells -- References -- Chapter 13. Rapid Microinjection of Fertilized Eggs -- I. Introduction -- II. Equipment -- III. Experimental Protocols -- References -- Chapter 14. Methods for Embryo Dissociation and Analysis of Cell Adhesion -- I. Introduction -- II. Gaining Access to the Egg and Embryo -- III. Cell Adhesion Assays: Nonquantitative and Semiquantitative -- IV. Quantitative Centrifugation Assay -- References -- Part III: Cell Biological Approaches -- Chapter 15. Analysis of Sea Urchin Embryo Gene Expression by Immunocytochemistry -- I. Introduction -- II. Immunocytochemistry on Whole Embryos -- III. Immunostaining Small Numbers of Embryos -- IV. Co-localization -- V. Troubleshooting -- References -- Chapter 16. Light Microscopy of Echinoderm Embryos -- I. Introduction (D. Burgess and L. Strickland) -- II. Formaldehyde Fixation of Cleavage Stage Sea Urchin Embryos -- III. Staining and Imaging Fixed Embryos -- IV. Simultaneous Fixation and Visualization of the Actin and Microtubule Cytoskeletons (G. von Dassow and V. Foe) -- V. Observation of Live Embryos (D. Burgess and L. Strickland) -- VI. 4-D Imaging of Fluorescent Markers in Live Starfish Oocytes ( J. Ellenberg and P. Lenart) -- References -- Chapter 17. TEM and SEM Methods -- I. Introduction -- II. Basic Fixation and Preparation -- III. Embedding Media -- IV. Preservation of Extracellular Matrix -- V. Fixation for SEM -- VI. Quick Freezing and Freeze-Substitution -- VII. Fixation for Immunohistochemistry and Immunocytochemistry -- VIII. Immunogold Methods -- IX. Ultrastructural Immunoperoxidase -- X. Preparation of Colloidal Gold Reagents (Horisberger, 1981 -- Slot and Geuze, 1985) as modified by Campbell (1990) and Reimer (1994) -- References -- Chapter 18. Calcium Imaging.
,
I. Introduction -- II. Calcium Signals in Embryos -- III. Getting Calcium Sensors into Embryos -- IV. Measuring Emitted Light -- V. Fluorescence Lifetime Imaging -- VI. Calibration -- VII. Manipulating Intracellular Calcium -- VIII. Conclusions and Perspectives -- References -- Chapter 19. Labeling of Cell Membranes and Compartments for Live Cell Fluorescence Microscopy -- I. Introduction -- II. Labeling of Extracellular Space/Endocytosis/Exocytosis -- III. Labeling of Plasma Membrane/Exocytosis/Endosomes -- IV. Labeling of the Cytosol -- V. Yolk Platelets/Reserve Granules -- VI. Mitochondria -- VII. Other Organelles -- VIII. Endoplasmic Reticulum -- IX. Golgi Apparatus -- X. Nucleus -- XI. Microscopy Considerations -- XII. Future Directions -- References -- Chapter 20. Isolation of Organelles and Components from Sea Urchin Eggs and Embryos -- I. Overview -- II. Egg Jelly Molecules Affecting Sperm -- III. Isolation of the Vitelline Layer from Sea Urchin Eggs -- IV. Isolation of the Cell Surface Complex and the Plasma Membrane-Vitelline Layer (PMVL) Complex from Sea Urchin Eggs -- V. The Cytolytic Isolation of the Egg Cortex -- VI. Isolation of Cortical Granules -- VII. Isolation of Yolk Platelets -- VIII. Isolation of Mitochondria from Eggs and Embryos -- IX. Isolation of Plasma Membranes and Lipid Rafts from Eggs and Zygotes -- X. Isolation of Microsomes Containing the Endoplasmic Reticulum -- XI. Nuclear Isolation Procedures -- XII. Removal and Isolation of the Fertilization Envelope -- XIII. Isolation of Cilia from Embryos -- XIV. Isolation of Extracellular Matrices from Embryos and Larvae -- XV. Isolation of Sea Urchin Larval Skeletons -- XVI. Resources for the Isolation of Additional Organelles -- References -- Chapter 21. Sea Urchin Spermatozoa -- I. Introduction -- II. Obtaining Sperm and Removing Coelomocytes.
,
III. Isolating Sperm Heads, Flagella, and Chromatin -- IV. The Isolated Sperm Flagellum as a Sealed Compartment -- V. Extracting Intact Sperm or Isolated Flagella and Heads with Detergents -- VI. Wheat Germ Agglutinin (WGA) Affinity Chromatography of Sperm Membrane Proteins -- VII. Isolating Sperm Plasma Membranes -- VIII. Isolation of Acrosome Reaction Vesicle Membranes (ARV) -- IX. Isolating Lipid Rafts from Sperm -- X. Scoring the Acrosome Reaction by Phase Contrast Microscopy -- XI. Sea Urchin Sperm Bindin -- References -- Chapter 22. Measuring Ion Fluxes in Sperm -- I. Overview -- II. Introduction -- III. Sperm Physiology is Deeply Influenced by Ion Channels and Transporters -- IV. Strategies to Study Sperm Ion Channels and Transporters -- References -- Part IV: Molecular Biological Approaches -- Chapter 23. Isolating DNA, RNA, Polysomes, and Protein -- I. Overview -- II. Introduction -- III. Purification of Total RNA -- IV. Purification of Cytoplasmic and Polysomal RNA -- V. Purification of Genomic DNA -- VI. Preparation of Protein Samples for One- and Two-Dimensional Gel Electrophoresis -- References -- Chapter 24. Detection of mRNA by In Situ Hybridization and RT-PCR -- I. Introduction -- II. In Situ Hybridization -- III. Quantitative PCR -- References -- Chapter 25. Using Reporter Genes to Study cis-Regulatory Elements -- Overview -- I. Introduction -- II. Using Chloramphenicol Acetyltransferase (CAT) Reporter Gene -- III. Using Green Fluorescent Protein (GFP) Reporter Gene -- IV. Quantitative Imaging of GFP in Living Embryos -- V. Using Luciferase (luc) Reporter Gene -- VI. Using lacZ Reporter Gene -- VII. Summary, Prospects, Concluding Remarks -- References -- Chapter 26. Identification of Sequence-Specific DNA Binding Proteins -- Overview -- I. Introduction -- II. Preparation of Nuclear Extracts.
,
III. Identification of Transcription Factor Target Sites by Footprint and Electrophoretic Mobility Shift Analysis (EMSA).
Permalink
