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  • 1
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA of Chenopodium album (L): a comparison of leaves and suspension cultures (1989)
    Springer
    Current genetics 16 (1989), S. 375-380 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondrial DNA ; Chenopodium album ; In vitro culture ; Mitochondrial plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial genome (mt genome) of Chenopodium album was analysed. It was found to have a size of about 270 kb and a GC content of 46%. The genomes of plant cells and suspension cultures were compared. Restriction fragment pattern analyses and hybridization experiments revealed quantitative and qualitative alterations. However, a comparison of the restriction fragment patterns of two independently established in vitro cultures did not reveal differences as far as the mt chromosomal DNA is concerned. Therefore, alterations in mtDNA induced by in vitro culture seem not to be caused by an entirely undirected process in Chenopodium. A plasmid-like DNA molecule was amplified in only one of the cultures investigated and not in the other or in the plant cells. This molecule has a length of 1,083 by and is referred to as Ca1 mp1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340718
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  • 2
    Electronic Resource
    Electronic Resource
    DNA sequence of a mitochondrial plasmid fromChenopodium album (1991)
    Springer
    Plant molecular biology 17 (1991), S. 155-156 
    Details
    ISSN: 1573-5028
    Keywords: mitochondrial plasmid ; mitochondrial genome ; Chenopodium album
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036820
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of the single psbA gene of Prochlorococcus marinus CCMP 1375 (Prochlorophyta) (1995)
    Springer
    Plant molecular biology 27 (1995), S. 1189-1196 
    Details
    ISSN: 1573-5028
    Keywords: Cyanobacteria ; gene copy number ; light regulation ; photosynthesis ; photosystem II reaction center ; polymerase chain reaction ; psbA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA sequence, copy number, expression and phylogenetic relevance of the psbA gene from the abundant marine prokaryote P. marinus CCMP 1375 was analyzed. The 7 amino acids near the C-terminus missing in higher plant and in Prochlorothrix hollandica D1 proteins are present in the derived amino acid sequence. P. marinus contains only a single psbA gene. Thus, this organism lacks the ability to adapt its photosystem II by replacement of one type of D1 by another, as several cyanobacteria do. Phylogenetic trees suggested the D1-1 iso-form from Synechococcus PCC 7942 as the next related D1 protein and place P. Marinus separately from Prochlorothrix hollandica among the cyanobacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020892
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning and sequencing of mutantpsbB genes of the cyanobacteriumSynechocystis PCC 6803 (1993)
    Springer
    Photosynthesis research 37 (1993), S. 139-146 
    Details
    ISSN: 1573-5079
    Keywords: chlorophyll-binding protein CP47 ; DNA sequence ; gene analysis ; mutagenesis ; Photosystem II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ten strains from a collection of mutants ofSynechocystis 6803 defective in Photosystem II (PS II) function were transformed with chromosomal DNA of wild-type and mutant cells. Cross hybridization data allowed to identify four groups of PS II-mutants. Highly efficient transformation was observed between different mutant groups, but not within the groups. Restoration of photosynthetic activity of the mutant cells was also achieved by transformation with different parts of a 5.6 kbBam HI fragment of wild typeSynechocystis DNA containing thepsbB gene. Each group of mutants was transformed to photoautotrophic growth by specific subfragments of thepsbB gene. DNA fragments of four selected mutant strains hybridizing with thepsbB gene were isolated and sequenced. The mutations were identified as a single nucleotide insertion or substitution leading to stop codon formation in two of the mutants, as a deletion of 12 nucleotides, or as a nucleotide substitution resulting in an amino acid substitution in the other two mutants. Deletion of 12 nucleotides in mutant strain PMB1 and stop codon formation in strain NF16 affect membrane-spanning regions of the gene product, the CP 47 protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02187472
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  • 5
    Electronic Resource
    Electronic Resource
    Transcription and in vivo expression of aMicrocystis aeruginosa plasmid (1990)
    Springer
    Current microbiology 20 (1990), S. 365-368 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A restriction map of the plasmid pMA1 ofMicrocystis aeruginosa is presented. The total RNA of the plasmid-containing strain HUB 5-2-4 was isolated and hybridized with the resident plasmid, pMA1, of the strain HUB 5-2-4. A transcript of 1800 b was detected. The minicell-producing strain X 984 ofEscherichia coli was transformed with pMA1 cloned into pUC18. Two polypeptides sized 50 kD and 43 kD respectively were synthesized in a specific manner in transformed cells; this indicates that the cyanobacterial transcription and translation signals are recognized byE. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02095861
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  • 6
    Electronic Resource
    Electronic Resource
    Plasmids in toxic and nontoxic strains of the cyanobacteriumMicrocystis aeruginosa (1988)
    Springer
    Current microbiology 17 (1988), S. 133-137 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The plasmid content and toxicity of nine different strains ofMicrocystis aeruginosa have been analyzed. The two toxic strains of the HUB Culture Collection were found to carry each two plasmids, pMA1 and pMA2, of 2.9 kb and 8.5 kb, respectively. In strains PCC 7813 and PCC 7820, also toxic, two different plasmids of 2.6 kb and 16 kb were detected. Hybridization experiments showed that there exists no sequence homology between the pMA plasmids and the plasmids found in the PCC strains; but the pMA plasmids hybridized to chromosomal DNA of the toxic strains PCC 7820, PCC 7813, HUB 063, and the nontoxic strain HUB 5-3. In nontoxic strains no or at most one plasmid of unstable occurrence could be detected. Only one of the toxic strains investigated, SAG 14.85 (NRC-1), contained no plasmid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01573468
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