Note: Front Cover -- Contents -- Preface -- Authors -- Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding -- Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR -- Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries -- Chapter 4: Subcloning of Genes or DNA Fragments -- Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing -- Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins -- Chapter 7: Characterization of DNA or Genes by Southern Blot Hybridization -- Chapter 8: Gene Overexpression by Sense RNA in Mammalian Systems -- Chapter 9: Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies -- Chapter 10: Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR -- Chapter 11: Analysis of Gene Expression at Proteomic Level via Western Blotting -- Chapter 12: Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization -- Chapter 13: Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization -- Chapter 14: In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs -- Chapter 15: Isolation and Characterization of Genes from Genomic DNA Libraries -- Chapter 16: Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression -- Chapter 17: Strategies for Gene Double Knockout -- Chapter 18: Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells -- Chapter 19: Quantitative Analysis of Functional Genome by Real-Time RT-PCR -- Chapter 20: High-Throughput Analysis of Gene Expression by Cutting-Edge Technology-DNA Microarrays (Gene Chips) -- Chapter 21: Construction and Screening of Human Antibody Libraries Using Phage Display Technology. , Chapter 22: Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology -- Chapter 23: Strategies for Gene Cloning, Expression, and Identification of Protein-Protein Interaction -- Chapter 24: Conditional Gene Knockout -- Chapter 25: How to Write a Research Manuscript for Publication in an English Journal -- Chapter 26: How to Protect Your Discovery and Invention : Patent 101 -- Chapter 27: Determination of Transgene Copy Numbers and Practical Biocalculation -- Back Cover.

Note: Intro -- NATIONAL WILDLAND FIRE MANAGEMENT STRATEGY -- NATIONAL WILDLAND FIRE MANAGEMENT STRATEGY -- CONTENTS -- PREFACE -- Chapter 1 A NATIONAL COHESIVE WILDLAND FIRE MANAGEMENT STRATEGY -- EXECUTIVE SUMMARY -- PART 1: NATIONAL CHALLENGES - MANAGING WILDFIRE ON THE NATIONAL LANDSCAPE -- National Challenges -- Engaging the Public -- More Resilient Landscapes in Dynamic Environments -- Roles and Responsibilities -- Agency and Mission Differences -- Building on Past Success and Shared Understanding -- Shared Assumptions -- PART 2: GUIDING PRINCIPLES AND CORE VALUES -- PART 3 : NATIONAL GOALS AND PERFORMANCE MEASURES -- Restore and Maintain Landscapes -- Fire Adapted Communities -- Wildfire Response -- Monitoring and Evaluation -- PART 4 : GOVERNANCE -- Overall Governance of the Cohesive Strategy -- Future Governance -- Regional Strategy Committees -- PART 5 -- Wildfire Risk Assessment Process -- Regional Strategies and Assessments -- Regions -- Comparing National and Regional Strategies -- Phase II: Development of Regional Strategies and Assessments -- Phase III: National Trade-off Analysis and Execution -- Conclusion -- APPENDIX A: COMPA RATIVE RISK ASSESSMENT -- A Comparative Risk Assessment Framework for Wildland Fire Management -- I. Background -- Essential Elements for Analyzing Risk -- Balancing Regional and National Priorities -- II. Probabilistic Assessment of Wildfire Risk: A National Example -- III. Exploring Options for Reducing Risk -- Option 1. Invest to Prevent Human Caused Ignitions -- Option 2. Invest in Fuel Treatments -- Option 3. Invest to Build Capacity in Fire Response -- Option 4. Invest to Protect Values Exposed to Risk -- IV. Risk Analyses at Smaller Spatial Scales -- Example Project Scale Prototype -- V. Historic Range of Variability for Wildfire Risk -- Sustainability -- Conclusions -- Literature Cited. , APPENDIX B: GLOSSARY -- Appendix C: Acronym List -- APPENDIX D: REFERENCES -- Cohesive Wildland Fire Management Strategy Foundational Documents -- References and Documents (1999-2009) -- APPENDIX E: WILDLAND FIRE LEADERSHIP COUNCIL MEMBERSHIP -- APPENDIX F: COHESIVE STRATEGY OVERSIGHT COMMITTEE -- APPENDIX G: PROJECT MANAGEMENT TEAM -- APPENDIX H: WILDLAND FIRE EXECUTIVE COUNCIL MEMBERSHIP -- APPENDIX I: PROCESS OVERVIEW -- Chapter 2 THE FEDERAL LAND ASSISTANCE, MANAGEMENT AND ENHANCEMENT ACT OF 2009: REPORT TO CONGRESS* -- EXECUTIVE SUMMARY -- INTRODUCTION -- THE FLAME ACT: THE NEXT STAGE IN THE PROCESS OF EVOLUTION -- FLAME ACT ELEMENT 1: MOST COST-EFFECTIVE MEANS FOR ALLOCATING BUDGET RESOURCES -- FLAME ACT ELEMENT 2: REINVEST IN NON-FIRE PROGRAMS -- FLAME ACT ELEMENT 3: ASSESSING RISK TO COMMUNITIES -- Identifying Risks -- Community Wildfire Protection Planning -- Fire-Adapted Communities -- Fire-Adapted Communities within a Cohesive Strategy -- Local Fit, National Programs -- All Must be Involved -- FLAME ACT ELEMENT 4: EMPLOY APPROPRIATE MANAGEMENT RESPONSE -- Local, State, Tribal and Federal Fire Suppression Partnership -- Protection Expectations and Responsibilities -- Guidance from Mutual Expectations for Preparedness and Suppression in the Interface -- Fire Prevention -- FLAME ACT ELEMENT 5: ALLOCATION OF HAZARDOUS FUEL REDUCTION FUNDING BASED ON PRIORITY PROJECTS -- Current State of Hazardous Fuel Reduction Projects -- Prioritization of Fuel Reduction Work -- Prioritization of Local Fuel Reduction Work -- Moving Forward -- Landscapes -- FLAME ACT ELEMENT 6: ASSESSING THE IMPACTS OF CLIMATE CHANGE ON THE FREQUENCY AND SEVERITY OF WILDFIRE -- Magnitude, Scope and Geographic Location of Impacts -- Climate, Wildfire, Biomass and Carbon Management Concerns -- Reducing Carbon Emissions -- Next Steps. , FLAME ACT ELEMENT 7: STUDY THE EFFECTS OF INVASIVE SPECIES ON WILDFIRE RISK -- Background -- Coordinated National Actions Are Needed -- Invasive Species Research Projects -- RECOMMENDED MANAGEMENT STRATEGIES -- Approaches for Addressing Wildfire -- Restore and Maintain Landscapes -- Estimated Costs Associated with Approaches -- Costs are not always Preceded by a Dollar Sign -- TRADE-OFFS ASSOCIATED WITH APPROACHES AND COSTS -- Evaluation of the Trade-Offs -- Regionalized Trade-Off Analyses -- In Summary -- Chapter 3 A COMPARATIVE RISK ASSESSMENT FRAMEWORK FOR WILDLAND FIRE MANAGEMENT: THE 2010 COHESIVE STRATEGY SCIENCE REPORT -- ABSTRACT -- ACKNOWLEDGMENTS -- 1. INTRODUCTION -- 2. THE COHESIVE STRATEGY -- 3. RISK FRAMEWORK -- 4. WILDFIRE RISK CASE STUDIES -- Fire Modeling -- Local-Scale Demonstration (Deschutes National Forest) -- Local-Scale Demonstration (Beaverhead-Deerlodge National Forest) -- Regional-Scale Demonstration -- National-Scale Demonstration Overview -- National Results Summary -- 5. COMPARATIVE RISK MITIGATION (EXPLORING OPTIONS FOR REDUCING RISK) -- Option 1. Invest to Prevent Human-Caused Ignitions -- Option 2. Invest in Fuel Treatments -- Project-Scale Fuels Treatment -- Forest-Level Fuels Treatment -- National Fuels Treatment -- Option 3. Invest to Build Capacity in Fire Response -- Option 4. Invest to Protect Values Exposed to Risk -- General Observations on Analyzing Options -- 6. OTHER RELATED EFFORTS -- Relation to the Previous Cohesive Strategy and Policy-Moving beyond FRCC -- Wildfire Ignition and its Relation to Risk -- Carbon and Emissions -- Relationship to Existing State and Regional Wildfire Risk Assessments -- Similarities and Differences with HFPAS Planning Efforts -- Comparison of Wildfire Risk Measures with National Insect and Disease Risk Map -- 7. SCIENCE LIMITATIONS -- Defining and Measuring Risk. , The Tipping Point Problem: Temporal Dynamics -- Accounting for a Changing Climate -- Quantifying Treatment Effectiveness -- Resource Value Measures -- Socioeconomic Vulnerability -- Smoke Management -- 8. POLICY ISSUES FOR TRADEOFF ANALYSIS -- DISCUSSION AND CONCLUDING REMARKS -- REFERENCES -- End Notes -- INDEX.