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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Cucumber production in Spain is being threatened by the cucumber vein yellowing virus (CVYV), which has been causing severe yield losses since it was first detected in 2000. Although most of the Spanish cucumber production is based on hybrid cultivars, farmers have grown cucumber landraces for centuries. A collection of 46 Spanish cucumber landraces, four melon cultivars and one accession of Cucumis africanus, were evaluated for resistance to CVYV. An isolate of CVYV from Almeria, closely related to CVYV from Israel, was used. CVYV was mechanically transmitted and detected by reverse transcriptase polymerase chain reaction and molecular hybridization. Only mild infections were observed in melons, while the wild species C. africanus was susceptible to this disease. Most of the cucumber accessions assayed were highly susceptible to CVYV and showed vein-clearing symptoms, severe growth reduction and a high viral accumulation. Several cucumber accessions were partially resistant to this virus, displaying mild symptoms and a significantly reduced viral accumulation. These partially resistant varieties could be cultivated under integrated production systems, and also be used as resistance sources to develop new cucumber hybrids resistant to CVYV.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 179 (1999), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ribotyping and RAPD profiling of a collection of 18 Streptococcus parauberis strains isolated from diseased turbot in Galicia (NW Spain) was performed in order to analyze the possible genetic variability within this bacterial fish pathogen. In addition, the value of this technique for intraspecific classification and epidemiological studies was evaluated. Ribopatterns of DNA digested with three endonucleases and hybridized with a cDNA probe complementary to highly conserved sequences in the 16S and 23S rRNA genes showed a great homogeneity among the turbot isolates. Compared with ribotyping, RAPD appeared to be a reliable and fast technique for discriminating between isolates of S. parauberis on the basis of their farm of isolation and, therefore, represents a powerful tool for epidemiological studies of this fish pathogen.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 65 (2000), S. 465-469 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5117
    Keywords: trace metals ; coastal marshes ; emergent macrophytes ; Schoenoplectus californicus ; Paraná River ; Río de la Plata Estuary
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cu, Cr, Pb, Zn and Fe in water, sediment and tissue of the emergent macrophyte Schoenoplectus californicus were studied in three coastal environments differing in aquatic chemistry and tide amplitude: a deltaic floodplain marsh, a coastal vegetated strip of the river and a tidal marsh at the south margin of the Río de la Plata Estuary. Metal content in water was not significantly different between the river and the floodplain marsh. Metal content in sediments showed higher values in the marshes than in the river, suggesting a retention in the marsh. Except for Cu, the highest metal content in sediments and water was observed in the tidal marsh. Pb in plants was undetectable. Metal content was higher in rhizomes, except Cr, which was mainly stored in stems. Rhizome metal content was higher in the river, probably related to the higher redox potential of its sediments. Inflorescence metal content was higher in the marshes: the highest Zn, Cr and Fe contents were measured in the tidal marsh; the highest Cu content was in the floodplain marsh, together with the highest organic matter content of the sediments. Zn, Cr and Fe increased in standing drying stems and in inflorescences in late fall. Inflorescences showed the highest metal content after seeds had been released.
    Type of Medium: Electronic Resource
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  • 5
    Publication Date: 2021-05-19
    Description: Realizado en el INIDEP (Instituto Nacional de Investigación y Desarrrollo Pesquero- Mar del Plata, Argentina)
    Description: Seminario Curso de Oceanografía Biológica (INIDEP, Mar del Plata).
    Description: ictioplancton, zooplancton, larvas de peces, abundancia, condiciones ambientales,
    Keywords: Zooplankton ; Fish larvae ; Environmental conditions ; Ichthyoplankton ; Zooplankton ; Fish larvae ; Abundance ; Environmental conditions
    Repository Name: AquaDocs
    Type: Theses and Dissertations
    Format: 18
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  • 6
    Publication Date: 2012-09-28
    Description: Regulation of the tumor suppressor PTEN by SUMO Cell Death and Disease 3, e393 (September 2012). doi:10.1038/cddis.2012.135 Authors: J González-Santamaría, M Campagna, A Ortega-Molina, L Marcos-Villar, C F de la Cruz-Herrera, D González, P Gallego, F Lopitz-Otsoa, M Esteban, M S Rodríguez, M Serrano & C Rivas
    Keywords: PTENSUMOVSV
    Electronic ISSN: 2041-4889
    Topics: Biology , Medicine
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  • 7
    Publication Date: 2015-06-23
    Description: Aims The physiological determinants of RV diastolic function remain poorly understood. We aimed to quantify the contribution of elastic recoil to RV filling and determine its sensitivity to interventricular interaction. Methods and results High-fidelity pressure–volume loops and simultaneous 3-dimensional ultrasound sequences were obtained in 13 pigs undergoing inotropic modulation, volume overload, and acute pressure overload induced by endotoxin infusion. Using a validated method, we isolated elastic restoring forces from ongoing relaxation using conventional pressure–volume data. The RV contracted below the equilibrium volume in 〉75% of the data sets. Consequently, elastic recoil generated strong sub-atmospheric passive pressure at the onset of diastole [–3 (–4 to –2) mmHg at baseline]. Stronger restoring suction pressure was related to a shorter isovolumic relaxation period, a higher rapid filling fraction, and lower atrial pressures (all P 〈 0.05). Restoring forces were mostly determined by the position of operating volumes around the equilibrium volume. By this mechanism, the negative inotropic effect of beta-blockade reduced and sometimes abolished restoring forces. During acute pressure overload, restoring forces initially decreased, but recovered at advanced stages. This biphasic response was related to alterations of septal curvature induced by changes in the diastolic LV–RV pressure balance. The constant of elastic recoil was closely related to the constant of passive stiffness ( R = 0.69). Conclusion The RV works as a suction pump, exploiting contraction energy to facilitate filling by means of strong elastic recoil. Restoring forces are influenced by the inotropic state and RV conformational changes mediated by direct ventricular interdependence.
    Print ISSN: 0008-6363
    Electronic ISSN: 1755-3245
    Topics: Medicine
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  • 8
    Publication Date: 2014-03-02
    Description: Vortices may have a role in optimizing the mechanical efficiency and blood mixing of the left ventricle (LV). We aimed to characterize the size, position, circulation, and kinetic energy (KE) of LV main vortex cores in patients with nonischemic dilated cardiomyopathy (NIDCM) and analyze their physiological correlates. We used digital processing of color-Doppler images to study flow evolution in 61 patients with NIDCM and 61 age-matched control subjects. Vortex features showed a characteristic biphasic temporal course during diastole. Because late filling contributed significantly to flow entrainment, vortex KE reached its maximum at the time of the peak A wave, storing 26 ± 20% of total KE delivered by inflow (range: 1–74%). Patients with NIDCM showed larger and stronger vortices than control subjects (circulation: 0.008 ± 0.007 vs. 0.006 ± 0.005 m 2 /s, respectively, P = 0.02; KE: 7 ± 8 vs. 5 ± 5 mJ/m, P = 0.04), even when corrected for LV size. This helped confining the filling jet in the dilated ventricle. The vortex Reynolds number was also higher in the NIDCM group. By multivariate analysis, vortex KE was related to the KE generated by inflow and to chamber short-axis diameter. In 21 patients studied head to head, Doppler measurements of circulation and KE closely correlated with phase-contract magnetic resonance values (intraclass correlation coefficient = 0.82 and 0.76, respectively). Thus, the biphasic nature of filling determines normal vortex physiology. Vortex formation is exaggerated in patients with NIDCM due to chamber remodeling, and enlarged vortices are helpful for ameliorating convective pressure losses and facilitating transport. These findings can be accurately studied using ultrasound.
    Print ISSN: 0363-6135
    Electronic ISSN: 1522-1539
    Topics: Medicine
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  • 9
    Publication Date: 2014-01-22
    Description: Background— Noninvasive indices based on Doppler echocardiography are increasingly used in clinical cardiovascular research to evaluate left ventricular global systolic chamber function. Our objectives were to clinically validate ultrasound-based methods of global systolic chamber function to account for differences between patients in conditions of abnormal load, and to assess their sensitivity to load confounders. Methods and Results— Twenty-seven patients (8 dilated cardiomyopathy, 10 normal ejection fraction, and 9 end-stage liver disease) underwent simultaneous echocardiography and left heart catheterization with pressure-conductance instrumentation. The reference index, maximal elastance ( E max ), was calculated from pressure–volume loop data obtained during acute inferior vena cava occlusion. A wide range of values were observed for left ventricular systolic chamber function ( E max : 2.8±1.0 mm Hg/mL), preload, and afterload. Among the noninvasive indices tested, the peak ejection intraventricular pressure difference showed the best correlation with E max ( R =0.75). A significant but weaker correlation with E max was observed for ejection fraction ( R =0.41), midwall fractional shortening ( R =0.51), global circumferential strain ( R =–0.53), and strain rate ( R =–0.46). Longitudinal strain and strain rate failed to correlate with E max , as did noninvasive single-beat estimations of this index. Principal component and multiple regression analyses demonstrated that peak ejection intraventricular pressure difference was less sensitive to load, whereas ejection fraction and longitudinal strain and strain rate were heavily influenced by afterload. Conclusions— Current ultrasound methods have limited accuracy to characterize global left ventricular systolic chamber function in a given patient. The Doppler-derived peak ejection intraventricular pressure difference should be preferred for this purpose because it best correlates with the reference index and is more robust in conditions of abnormal load.
    Keywords: Contractile function, Cardiovascular imaging agents/Techniques, Echocardiography
    Print ISSN: 1941-9651
    Electronic ISSN: 1942-0080
    Topics: Medicine
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