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  • 1
    Electronic Resource
    Electronic Resource
    Toxicity of a soluble peptide from Microcystis sp. to zooplankton and fish (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 24 (1990), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 〈list xml:id="l1" style="custom"〉1 A soluble cell fraction lethal to Daphnia magna was isolated by the disruption of net phytoplankton from eutrophic Aculeo Lake. The phytoplankton of this lake is dominated by Microcystis sp.2 The soluble fraction was also lethal to common native zooplankton, including the rotifers Keratella sp., Trichocerca similis, the copepod Boeckella sp., and the cladoceran Chydorus sphaericus. In addition, a partially purified soluble component of the lethal fraction caused the death of the fish Gambusia affinis.3 The specific toxicity of the soluble fraction from phytoptankton varied four- to six-fold seasonally with peaks during periods of warm temperature.4 Our results suggest that releases of a toxic peptide from Microcystis sp. may be the cause of massive fish kills at the study site, Aculeo Lake.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2427.1990.tb00705.x
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Spawning of Basilichthys microlepidotus (Jenyns) (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 41 (1992), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Characteristics of the reproductive cycle of Basilichthys microlepidotus (Jenyns), an atherinid fish living in a South American Mediterranean arid zone, are analysed. Specimens 9-170 mm in total length were captured from August 1982 to April 1984 in the Rio Choapa, in northern Chile (31%%45′ S, 71°15′ W). Analysis of gonadosomatic index fluctuations, and determination of the proportion of mature males and females captured during the study period, allowed the estimation of the duration of the reproductive cycle.Females 70–170 mm in total length contained 2000–9000 intraovarian oocytes, of which approximately 80% were vitellogenic (100–1300 μm in diameter) and exhibited a polymodal distribution. A further 4 to 15% of the total oocytes, measured 1700–2000 μm in diameter and appeared ready to be spawned. In 30% of the examined ovaries some of the largest oocytes exhibited signs of degeneration, or atresia. Juveniles approximately 30 mm in total length were captured over most of the study period. Results strongly suggest that B. microlepidotus has multiple spawnings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1992.tb02724.x
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    Paper (German National Licenses)
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  • 3
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    Diversidad de bacterias fototrofas y evidencia del clado Roseobacter en el Salar de Huasco, un humedal salino de altura del Altiplano Chileno (2008)
    In:  [Poster] In: XXX. Congreso Chileno de Microbiología, 04.-06.12, Concepción, Chile .
    Details
    Publication Date: 2012-02-23
    Type: Conference or Workshop Item , NonPeerReviewed
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    OceanRep: Poster
  • 4
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    High Archael Diversity in a high altitude saline wetland (2006)
    In:  [Poster] In: 11th International Symposium on Microbial Ecology (ISME 11), 20.-25.08, Vienna, Austria .
    Details
    Publication Date: 2012-02-23
    Type: Conference or Workshop Item , NonPeerReviewed
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  • 5
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    Microbial diversity in high altitude wetlands: the role of nitrifying bacteria in Salar de Huasco, northern Chile (2006)
    In:  [Talk] In: AQUAVIT-Seminar, 02.03, Plön .
    Details
    Publication Date: 2012-02-23
    Type: Conference or Workshop Item , NonPeerReviewed
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    OceanRep: Talk
  • 6
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    Diversity of Bacteroidetes in high altitude saline evaporitic basins in northern Chile (2009)
    AGU (American Geophysical Union)
    In:  Journal of Geophysical Research: Biogeosciences, 114 . G00D05.
    Details
    Publication Date: 2019-09-23
    Description: The phylum Bacteroidetes represents one of the most abundant bacterial groups of marine and freshwater bacterioplankton. We investigated the diversity of Bacteroidetes in water and sediment samples from three evaporitic basins located in the highlands of northern Chile. We used both 16S rRNA gene clone libraries created with targeted Bacteroidetes-specific primers and separation of specifically amplified gene fragments by denaturing gradient gel electrophoresis (DGGE). DGGE analysis revealed a reduced richness of these organisms in samples from Salar de Huasco (two to four DGGE bands) increasing in Salar de Ascotán (two to seven DGGE bands) and Laguna Tebenquiche at Salar de Atacama (four to eight DGGE bands). Cluster analysis (WPGMA) of DGGE bands showed that bands from Salar de Huasco and Salar de Ascotán grouped together and samples from Salar de Atacama formed separate clusters in water and sediment samples, reflecting different Bacteroidetes communities between sites. Most of the sequences analyzed belonged to the family Flavobacteriaceae and clustered with the genera Psychroflexus, Gillisia, Maribacter, Muricauda, Flavobacterium, and Salegentibacter. The most abundant phylotype was highly related to Psychroflexus spp. and was recovered from all three study sites. The similarity of the analyzed sequences with their closest relatives in GenBank was typically 〈97% and notably lower when compared with type strains, demonstrating the unique character of these sequences. Culture efforts will be necessary to get a better description of the diversity of this group in saline evaporitic basins of northern Chile.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1029/2008JG000837
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  • 7
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    Cyanobacterial diversity in Salar de Huasco, a high altitude saline wetland in Northern Chile, are highly similar to Antarctic cyanobacteria (2008)
    Oxford University Press
    In:  FEMS Microbiology Ecology, 64 . pp. 419-432.
    Details
    Publication Date: 2019-09-23
    Description: The diversity of Cyanobacteria in water and sediment samples from four representative sites of the Salar de Huasco was examined using denaturing gradient gel electrophoresis and analysis of clone libraries of 16S rRNA gene PCR products. Salar de Huasco is a high altitude (3800 m altitude) saline wetland located in the Chilean Altiplano. We analyzed samples from a tributary stream (H0) and three shallow lagoons (H1, H4, H6) that contrasted in their physicochemical conditions and associated biota. Seventy-eight phylotypes were identified in a total of 268 clonal sequences deriving from seven clone libraries of water and sediment samples. Oscillatoriales were frequently found in water samples from sites H0, H1 and H4 and in sediment samples from sites H1 and H4. Pleurocapsales were found only at site H0, while Chroococcales were recovered from sediment samples of sites H0 and H1, and from water samples of site H1. Nostocales were found in sediment samples from sites H1 and H4, and water samples from site H1 and were largely represented by sequences highly similar to Nodularia spumigena. We suggest that cyanobacterial communities from Salar de Huasco are unique - they include sequences related to others previously described from the Antarctic, along with others from diverse, but less extreme environments.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1111/j.1574-6941.2008.00483.x
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  • 8
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    Molecular analysis of enrichment cultures of ammonia oxidizers from the Salar de Huasco, a high altitude saline wetland in northern Chile (2008)
    Springer
    In:  Extremophiles, 12 . pp. 405-414.
    Details
    Publication Date: 2019-09-23
    Description: We analyzed enrichment cultures of ammonia-oxidizing bacteria (AOB) collected from different areas of Salar de Huasco, a high altitude, saline, pH-neutral water body in the Chilean Altiplano. Samples were inoculated into mineral media with 10 mM NH4+ at five different salt concentrations (10, 200, 400, 800 and 1,400 mM NaCl). Low diversity (up to three phylotypes per enrichment) of beta-AOB was detected using 16S rDNA and amoA clone libraries. Growth of beta-AOB was only recorded in a few enrichment cultures and varied according to site or media salinity. In total, five 16S rDNA and amoA phylotypes were found which were related to Nitrosomonas europaea/Nitrosococcus mobilis, N. marina and N. communis clusters. Phylotype 1-16S was 97% similar with N. halophila, previously isolated from Mongolian soda lakes, and phylotypes from amoA sequences were similar with yet uncultured beta-AOB from different biofilms. Sequences related to N. halophila were frequently found at all salinities. Neither gamma-AOB nor ammonia-oxidizing Archaea were recorded in these enrichment cultures.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1007/s00792-008-0146-x
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  • 9
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    High-Fat Diet-Mediated Lipotoxicity and Insulin Resistance Is Related to Impaired Lipase Expression in Mouse Skeletal Muscle (2013)
    Oxford University Press
    Details
    Publication Date: 2013-03-23
    Description: Elevated expression/activity of adipose triglyceride lipase (ATGL) and/or reduced activity of hormone-sensitive lipase (HSL) in skeletal muscle are causally linked to insulin resistance in vitro. We investigated here the effect of high-fat feeding on skeletal muscle lipolytic proteins, lipotoxicity, and insulin signaling in vivo. Five-week-old C3H mice were fed normal chow diet (NCD) or 45% kcal high-fat diet (HFD) for 4 weeks. Wild-type and HSL knockout mice fed NCD were also studied. Whole-body and muscle insulin sensitivity, as well as lipolytic protein expression, lipid levels, and insulin signaling in skeletal muscle, were measured. HFD induced whole-body insulin resistance and glucose intolerance and reduced skeletal muscle glucose uptake compared with NCD. HFD increased skeletal muscle total diacylglycerol (DAG) content, protein kinase C and protein kinase C membrane translocation, and impaired insulin signaling as reflected by a robust increase of basal Ser1101 insulin receptor substrate 1 phosphorylation (2.8-fold, P 〈 .05) and a decrease of insulin-stimulated v-Akt murine thymoma viral oncogene homolog Ser473 (–37%, P 〈 .05) and AS160 Thr642 (–47%, P 〈.01) phosphorylation. We next showed that HFD strongly reduced HSL phosphorylation at Ser660. HFD significantly up-regulated the muscle protein content of the ATGL coactivator comparative gene identification 58 and triacylglycerol hydrolase activity, despite a lower ATGL protein content. We further show a defective skeletal muscle insulin signaling and DAG accumulation in HSL knockout compared with wild-type mice. Together, these data suggest a pathophysiological link between altered skeletal muscle lipase expression and DAG-mediated insulin resistance in mice.
    Print ISSN: 0013-7227
    Topics: Medicine
    Published by Oxford University Press on behalf of The Endocrine Society.
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