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  • 1
    Book
    Book
    Hydrostatic pressure effects on photosynthesis (1968)
    Ann Arbor, Mi. : UMI
    Details Availability
    Type of Medium: Book
    Pages: VII, 129 S , Ill.
    Language: Undetermined
    Note: Stanford Univ., Diss. 1964. -
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  • 2
    Electronic Resource
    Electronic Resource
    Resistance to photodamage in evergreen conifers (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 80 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Conifers in the temperate zone are subject to extremes in climatic conditions, such as low temperatures and water deficits. Under such conditions chloroplast antenna pigments can absorb more light energy than can be utilized in photosynthesis. If the plant is unable to dissipate this excess energy by combined radiationless decay (heat), fluorescence emission and carotenoid quenching, photodamage can result. Potentially damaging conditions exist during winter when low temperatures often occur simultaneously with intense light levels and desiccation. Photodamage is caused when regulatory controls on the production of toxic oxygen species produced in the chjoroplast and the capacity of scavenging systems to dispose of them are exceeded. By becoming cold-hardened and dormant, conifer species can resist all but the most severe effects of the winter climates to which they are adapted. Mechanisms which protect chloroplasts from photodamage appear to be among the essential adaptations enabling temperate conifers to resist the effects of drought and low winter temperatures, especially when light levels are high.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb04389.x
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  • 3
    Electronic Resource
    Electronic Resource
    Plantlet morphology and the regulation of net water loss in tissue-cultured Douglas-fir (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 83 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Tissue-culture plantlets of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] were highly susceptible to detrimental water loss upon removal from culture in vitro. Control of net water loss was related to shoot and root morphology. Relative water content after 3 h of atmospheric water stress was positively correlated to root number, root surface area, and the length of the longest root, and was inversely correlated to the ratio of needle surface area/root surface area. High relative water content apparently was a result of a higher rate of water uptake among plantlets with beneficial morphological features. It is recommended that, to improve the ability of a plantlet to withstand water stress during acclimatization, beneficial root system features be focused upon during plantlet production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb01289.x
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  • 4
    Electronic Resource
    Electronic Resource
    Photosynthetic competence of plantlets grown in vitro. An automated system for measurement of photosynthesis in vitro (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 84 (1992), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An aseptic gas exchange and hydroponic system (AGEHS) has been developed in an attempt for characterization of physiological requirements for photoautotrophic growth in vitro and alleviation of the needs for ex vitro acclimatization. The AGEHS monitors and controls several parameters relevant to plant growth. Shootlets of Chrysanthemum × morifolium Ramat. cv. Envy were treated with flow of air or CO2-enriched air under controlled relative humidity, elevated photosynthetic photon flux density (PPFD) and hydroponic irrigation. After 15 days of treatment, plantlets gained more than 3 times as much dry weight as those from a conventional culture tube treatment. This study shows that it is possible to favour photoautotrophic growth when elevated PPFD, enhanced air-exchange and hydroponic medium flow are provided concurrently. This enhancement is achievable through careful increments of light quanta, balanced with increments of humidified air flow and/or CO2 content in air which seem to be necessary to avoid potential photoinhibition and premature water exhaustion from gelled media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1992.tb04684.x
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  • 5
    Electronic Resource
    Electronic Resource
    Root production and plantlet development in tissue-cultured conifers (1988)
    Springer
    Plant cell, tissue and organ culture 14 (1988), S. 137-160 
    Details
    ISSN: 1573-5044
    Keywords: conifers ; tissue culture ; micropropagation ; adventitious rooting ; acclimatization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rooting and acclimatization procedures for micropropagated conifers are reviewed, with emphasis on their effects on root quality and plantlet performance in the nursery and field. Major influences on root production include auxin concentration and mode of application, shoot quality, donor age, clone and temperature. The development of a fibrous, well-branched root system has been a problem that may be solved by using rooting substrates that are better-aerated than agar. Further development of the root system may be enhanced by early air-pruning and ectomycorrhizal associations. During acclimatization, high humidity is required for conifers. However, conifers have an advantage over non-coniferous plantlets with respect to water loss because of a better development of the needle cuticles prior to transfer to in vivo conditions. In greenhouse and field comparisons with seedlings, plantlets were similar in survival and growth rate, but root systems were less fibrous. Also, features of early maturation have been observed for plantlets, the cause of which is uncertain. Pertinent research with rooted cuttings and seedlings of conifers has been cited to gain a better understanding of the factors involved in root production and development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043405
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  • 6
    Electronic Resource
    Electronic Resource
    Fixation of 14CO2 in tissue cultured red raspberry prior to and after transfer to soil (1984)
    Springer
    Plant cell, tissue and organ culture 3 (1984), S. 313-317 
    Details
    ISSN: 1573-5044
    Keywords: acclimatization ; tissue culture ; 14CO2 ; photosynthesis ; light ; Rubus idaeus L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 14CO2 uptake of an aseptically cultured red raspberry clone (Rubus ideaus L.) was examined prior to and after transfer to soil. Individual leaves of transplants, both persistent from culture and new ones, were tested 5 weeks after transplant for 14CO2 uptake capability. Transplant leaves of successive weekly age classes took up 14CO2 at increasing rates per unit area, displaying a spectrum of photosynthetic competence from low levels close to that of leaves from culture, to that of control plants. This is illustrative of acclimatization to the soil environment and was related to transplant light intensity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043082
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  • 7
    Electronic Resource
    Electronic Resource
    The anatomy of tissue cultured red raspberry prior to and after transfer to soil (1985)
    Springer
    Plant cell, tissue and organ culture 3 (1985), S. 43-50 
    Details
    ISSN: 1573-5044
    Keywords: tissue culture ; anatomy ; transplantation ; acclimatization ; light ; Rubus idaeus L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The leaf, petiole, stem and root anatomy of an aseptically cultured red raspberry clone (Rubus idaeus L.) was studied before and 5 weeks after transfer to soil under controlled environmental conditions. Tissues persistent from culture showed little or no change with time in soil; they grew minimally and slight secondary wall deposition occurred. New organs formed in successive weeks after transplantation showed a graded increase in potential size and development. Some features, such as collenchyma formation, rapidly returned to control levels; this was seen in new leaves expanding in the first week after transplantation. Other features, such as sclerenchyma formation, did not occur in leaves expanding during the first 2 weeks after transplantation, even when these were a month or more in age. Some sclerenchyma was seen in leaves expanding in the third week after transplantation, increasing in later-formed leaves. Increasing the light intensity of transplant accelerated the return to control-type organ size and appearance. During acclimatization transitional forms of leaves, petioles, stems and roots develop that ranged anatomically from culture-to control-type. This trend is analagous to the normal developmental sequence of organ formation as it affects the potential for development of successily formed organs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041654
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  • 8
    Electronic Resource
    Electronic Resource
    Micropropagation of yellow cedar (Chamaecyparis nootkatensis) (1989)
    Springer
    Plant cell, tissue and organ culture 18 (1989), S. 297-312 
    Details
    ISSN: 1573-5044
    Keywords: yellow cedar ; Chamaecyparis nootkatensis ; culture media ; micropropagation ; N6-benzyladenine ; organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mature yellow cedar (Chamaecyparis nootkatensis (D. Don) Spach) embroys were exposed to a range of N6-benzyladenine concentrations in a variety of culture media generally used for conifer caulogenesis. All seven media supported the induction of adventitious shoots but Schenk & Hildebrandt medium was the best. The best cytokinin level of N6-benzyladenine was 0.35 mg 1-1. This resulted in an average of 4–5 large adventitious shoots per explant. Shoots arose primarily from the cotyledons regardless of whether they were in contact with the medium or not. Embryos from seeds stratified four weeks at 21°C and eight weeks at 5°C were more caulogenic than unstratified controls. An additional four weeks at 5°C caused a change in the pattern of shoot induction in that shoots arose from the hypocotyl as well as the cotyledons. Shoots elongated on basal Schenk & Hildebrandt medium. The best rooting response was obtained under non-sterile greenhouse conditions where approximately 60% of the shoots formed roots. Over a 12-month period the average shoot height ranged between 10–13.9 cm with a stem diameter of 2.29–2.68 mm. These propagules are still being grown under forest nursery conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043399
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  • 9
    Electronic Resource
    Electronic Resource
    The influence of acclimatization treatment and plantlet morphology on early greenhouse-performance of tissue-cultured Douglas fir [Pseudotsuga menziesii (Mirb.) Franco] (1990)
    Springer
    Plant cell, tissue and organ culture 21 (1990), S. 111-117 
    Details
    ISSN: 1573-5044
    Keywords: tissue culture ; acclimatization ; plantlet quality ; adventitious rooting ; Douglas fir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this investigation was to test for the possible effects of plantlet morphology and environmental stress on survival and growth during the acclimatization of tissue-cultured Douglas fir [Pseudotsuga menziesii (Mirb.) Franco]. Under a high-stress environmental regime of 40–70% relative humidity and 22–28°C, survival was 33%, compared to 89% under a low-stress regime of 80–90% RH and 15–20°C. Shoot elongation under low stress was twice as great as that under high stress. Certain morphological features were associated with improved survival under high stress, and they included a large (10+) root number, tall (40 + mm) shoots, and upright needles. Plantlets with 10 + roots had 52% survival, compared to 25% or 31% for those with 1–3 or 4–10 roots, respectively. Also, plantlets with an initial shoot height of 41–60 mm had a 53% survival rate compared to 20% or 37% for those with 21–30 mm or 31–40 mm shoots. Tall plantlets and those with 10+ roots also underwent the greatest shoot elongation during the 7 week observation period. However, plagiotropism was frequent on tall shoots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033429
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