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  • 1
    Online Resource
    Online Resource
    Analytische Elektronenmikroskopie in Molekularbiologie und Biotechnologie : Schlussbericht (2002)
    Oberkochen : LEO Elektronenmikroskopie
    Details Availability
    Keywords: Forschungsbericht ; Gen ; Elektronenmikroskopie
    Description / Table of Contents: ESI, compositional contrast, boron labelling, ferritin, double immunolabelling, oligonucleotide, slowscan CCD, Omega filter, SESAMe 1, field emission
    Type of Medium: Online Resource
    Pages: Online-Ressource (115 S., 14,1 MB) , Ill., graph. Darst
    URL: https://edocs.tib.eu/files/e01fb04/470805587.pdf
    URL: https://doi.org/10.2314/GBV:470805587
    URL: https://edocs.tib.eu/files/e01fb04/470805587l.pdf
    DOI: 10.2314/GBV:470805587
    Language: German
    Note: Förderkennzeichen BMBF 0310944A. - Engl. Zsfassung u.d.T.: Analytical electron microscopy in molecular biology and biotechnology , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden , Auch als gedr. Ausg. vorhanden , Systemvoraussetzungen: Acrobat reader.
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  • 2
    Electronic Resource
    Electronic Resource
    Structural organization of an active, chromosomal nucleolar organizer region (NOR) identified by light microscopy, and subsequent TEM and STEM electron microscopy (1985)
    Springer
    Chromosoma 91 (1985), S. 151-163 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The three-dimensional arrangement of the chromatin components within the nucleolar organizer regions (NORs) from living oocyte nuclei was investigated. As a suitable cell system we chose vitellogenic oocytes of the orthopteran insect Acheta. This cell type is particularly attractive for analysis of nucleolar chromatin, since structural and functional aspects of NORs during early oogenic stages (including the association of NORs with amplified rDNA copies) are particularly well known (Lima-de-Faria 1974). In the course of the present study we first identified putative chromosomal NORs in isolated nuclei of mid-diplotene oocytes according to morphological characteristics using differential interference contrast (DIC) or phase contrast light microscopy. The presence of actively transcribing chromosomal NORs during this late stage of Acheta oogenesis obviously had been overlooked by previous investigators, probably due to difficulties of chromosome visualization. For a more detailed ultrastructural analysis, NORs were gently sedimented from opened nuclei and processed for sectioning using a modified “end-embedding” procedure (Mott and Callan 1975; Spring and Franke 1981). A small number of thick and thin sections could be made from individual NORs. Sections were analyzed by light microscopy, conventional transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM). Whereas the structural connection of NORs to the chromosome axis and also the general arrangement of active nucleolar genes within the NOR complex could be seen with TEM, the visualization of individual nucleolar genes and the organization of transcription complexes was only possible using bright field STEM of thick sections at low temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294060
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Restriction analysis of λEMBL3 background recombinants: Occurrence of λ phages carrying a head to tail oriented left arm DNA sequence (1989)
    Springer
    Molecular genetics and genomics 217 (1989), S. 533-535 
    Details
    ISSN: 1617-4623
    Keywords: λEMBL3 ; Recombinant background ; BamHI cloning ; Restriction endonuclease analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Eight representative recombinant background clones of λEMBL3 were analysed usingKpnI,BamHI,SalI,EcoRI andHindIII digestion. We found that λEMBL3 carries its own left arm in theBamHI cloning site. In this way, recombinant molecules were found to be generated which can grow onEscherichia coli strain NM539. In all cases analysed, the left arm DNA was inserted in a head to tail orientation. Seven clones carried a restoredBamHI site at thecos site-BamHI site connection. In the region where the inserted left arm and the right arm were ligated,BamHI cloning produces a large palindromic sequence consisting of two polylinkers. ThisBamHI site was incompletely cleaved in all cases analysed. We assume that a part of the λ DNA molecule in this region shows a cruciform structure prohibiting recognition or cleavage of this site by restriction endonucleaseBamHI.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02464928
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  • 4
    Electronic Resource
    Electronic Resource
    The autoantigen La/SS-B: analysis of the expression of alternatively spliced La mRNA isoforms (1996)
    Springer
    Cell & tissue research 284 (1996), S. 383-389 
    Details
    ISSN: 1432-0878
    Keywords: Key words: SS-B/La protein ; La cDNA ; Autoimmunity ; In situ hybridization ; Gene expression ; Endothelium ; Blood vessels ; Liver ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The gene for the nuclear autoantigen La/SS-B encodes two La mRNA isoforms. In order to study the function and expression of both La mRNA forms, an in situ hybridization procedure was developed allowing the selective identification of either exon 1 or exon 1′. For this purpose, digoxigenin-labeled exon-specific sense and anti-sense probes were prepared by in vitro transcription from plasmids that contained the respective exon sequence. Detection of the probes was carried out by using rhodamine-conjugated anti-digoxigenin antibody and confocal laser scanning microscopy. Both La mRNAs were found in the cytoplasm of endothelial cells but not in smooth muscle cells. In addition to the in situ technique, an assay system was established allowing the expression ratio of the two mRNA forms to be determined. The estimation was based on the amplification of exon 1 and 1′ La cDNAs in parallel by using a three primer polymerase chain reaction. The ratio of the exon 1 to exon 1′ La mRNA forms was determined to be about 5:1 in liver tissue and endothelial cells. The data support the conclusion that both La mRNA forms represent finally processed cytoplasmic mRNAs that are up- or downregulated in parallel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050599
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  • 5
    Electronic Resource
    Electronic Resource
    Über 3-Hydroxy-fluoranthen-carbonsäuren-(1), -(2) und -(10) (1962)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 95 (1962), S. 3013-3029 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Durch Behandlung mit Luft in alkalischem Milieu werden Carbonsäurederivate des 3-Oxo-1.2.3.lob-tetrahydro-fluoranthens in entsprechende 3-Hydroxy-fluoranthencarbonsäuren und ihre Abkömmlinge übergeführt. Durch Belichtung von 3-Hydroxy-fluoranthen entsteht 1-Carboxy-fluoren-essigsäure-(9). Entsprechend wird aus 3-Hydroxy-fluoranthen-carbonsäure-(1) 1-Carboxy-fluoren-malonsäure-(9) gebildet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19620951227
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  • 6
    Electronic Resource
    Electronic Resource
    Über 3-Hydroxy-fluoranthene (1963)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 96 (1963), S. 2577-2594 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 3-Oxo-1.2.3.10b-tetrahydro-fluoranthen und seine Derivate werden mit Luft in alkalischem Medium zu 3-Hydroxy-fluoranthenen aromatisiert. Einige 3-Hydroxy-fluoranthene geben Molekülkomplexe mit Fluorenon-carbonsäure-(1). Die IR-Spektren dieser Komplexe und die IR-Spektren verschiedenfarbiger Fluorenoncarbonsäuren werden diskutiert. Die Überführung von 3-Hydroxy-fluoranthen in indigoide Farbstoffe wird beschrieben.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19630961009
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