Note: Front Cover -- Cell Separation: Methods and Selected Applications -- Copyright Page -- Table of Contents -- Contributors -- Preface -- Contents of Previous Volumes -- Chapter 1. Separation of Malignant Lymphoid Cells by Countercurrent Distribution -- I. Introduction -- II. Experimental Methods -- III. Fractionation of RAW117 Lymphoma Cells -- IV. Discussion of the Technique -- References -- Chapter 2. Use of Lectins for Separation of Cells -- I. Introduction -- II. Methodology -- III. Selected Applications -- IV. Concluding Remarks -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 3. Enzymatic Disaggregation of Human Bronchogenic Carcinomas followed by Velocity Sedimentation of Cells -- I. Introduction -- II. Enzymatic Disaggregation of Tumors -- III. Suspensions of Tumors: Storage and Composition -- IV. Purification of Cells from Lung Tumors -- V. Concluding Remarks -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 4. Macrophage Electrophoretic Migration (MEM) Test with Some Illustrative and Heuristic Applications -- I. Historical Introduction -- II. Basic Requirements -- III. Preparation of Macrophages -- IV. Preparation of Lymphocytes -- V. Procedure: Cancer -- VI. Applications -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 5. Implementation of Microtechniques to Resolve Tissue Heterogeneity -- I. Introduction -- II. Description of the Microtechniques -- III. Cell Differentiation in the Cellular Slime Mold Dictyostelium discoideum -- IV. Adaptation of the Micromethods to Human Mammary Tumors -- V. Conclusions -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 6. In Vitro Isolation of Malignant Cells from Small Cell Carcinomas -- I. Introduction -- II. Establishment of Cell Lines -- Ill, Characteristics of Cultured Cells -- IV. Summary -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 7.Computer-Controlled Cell Electrophoresis Microscope -- I. Introduction. , II. Design of the Automated Electrophoresis Microscope System (AEMS) -- III. Features Important to the Operation of the AEMS -- IV. Conclusions -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 8. Separation of Cells from the Rat Anterior Pituitary Gland -- I. Cellular Organization of the Rat Anterior Pituitary Gland -- II. Preparation of Rat Pituitary Cell Suspensions -- III. Methods Used to Differentiate Pituitary Cell Types -- IV. Review of Pituitary Cell Separation Studies -- V. Separated Pituitary Cells: Applications -- VI. Concluding Remarks -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 9. Isolation of Type II Pneumocytes and Their Application in the Study of Pulmonary Metabolism -- I. Introduction -- II. Overview-Type II Cell Isolation -- III. Method of Isolation -- IV. Applications -- V. Conclusion -- REFERENCES -- Chapter 10. Selective Media -- I. Introduction -- II. Biological Basis for Selective Growth -- III. Families of Cells with Similar Growth Requirements -- IV. Selective Growth of Human Epidermal Keratinocytes -- V. Techniques for Achieving Selective Growth -- VI. Future Prospects -- REFERENCES -- Chapter 11. Cell Labeling and Separation Using Immunospecific Miscrospheres -- I. Introduction -- II. General Considerations -- III. Cell-Specific Ligands -- IV. Microspheres for Cell Labeling and Separation -- V. Cell-Labeling Methods -- VI. Application of Microspheres in Cell Separations -- VII. Summary and Concluding Remarks -- REFERENCES -- Chapter 12. Collagenase and the Isolation of Cells from Bone -- I. Cells of the Osteoblast Line -- II. Cells of the Osteoclast Line -- III. Isolation of Calvarial Cells -- IV. Clostridium histolyticum Collagenase -- V. Cell Separation Techniques -- VI. Culture of Human Bone Cells -- VII. Freezing Cells for Storage -- REFERENCES -- Chapter 13. Cell Separation by Biological Methods -- I. Introduction. , II. Differential Culture Procedures -- III. Selective Reagents -- IV. Selective Media -- V. Discussion -- ACKNOWLEDGMENTS -- REFERENCES -- Index.

Note: Intro -- Preface -- Contents -- Acronyms -- 1 The Interstellar Medium -- 1.1 Overview -- 1.2 The Dusty ISM -- 1.3 Interstellar Radiation Field -- 1.4 Photodissociation Regions -- 1.5 Cosmic Rays -- 1.6 Detection of H2 -- 1.7 Life Cycle of the Stars and the ISM -- References -- 2 Nebulae -- 2.1 Ionization and Recombination of Hydrogen -- 2.2 Categories of Nebulae -- 2.2.1 Diffuse (Emission) Nebulae -- 2.2.2 Planetary Nebulae -- 2.2.3 Supernova Remnants -- 2.2.4 Reflection and Dark Nebulae -- 2.3 Photoionization Equilibrium -- 2.4 Heating and Cooling Mechanisms -- 2.5 R-Type Expansion -- 2.6 D-Type Expansion -- 2.6.1 Further Discussion -- References -- 3 Triggered Star Formation -- 3.1 Overview -- 3.2 Gravitational Collapse -- 3.3 Instabilities During the D-Type Phase -- 3.3.1 Vishniac Instability -- 3.3.2 Rayleigh-Taylor Instability -- 3.4 Collect and Collapse -- 3.5 Radiation-Driven Implosion -- 3.6 The Formation of Our Solar System -- References -- 4 Numerical Simulations -- 4.1 Introduction -- 4.2 Methods Followed in Numerical Simulations -- 4.2.1 Grid-Based Methods -- 4.2.2 Smoothed Particle Hydrodynamics Methods -- 4.2.3 Hybrid Methods -- 4.3 Simulations -- 4.3.1 Spherically Symmetric Expansion -- 4.3.2 Off-Center Expansion -- 4.3.3 Radiation Driven Implosion -- 4.3.4 Fractal Clouds -- 4.4 Synthetic Observations -- References -- 5 Summary.

Note: Cover -- Contents -- Acknowledgements -- Notes on Contributors -- Introduction -- 1 Sociology and the Sociological Imagination: Reflections on, Disciplinarity and Intellectual Specialisation -- 2 Nouns and Verbs: Old and New Strategies for Sociology -- 3 Sociological Theory: Still Going Wrong? -- 4 A Plea for Earthly Sciences -- 5 Complexity, 'Nature' and Social Domination: Towards a Sociology of Species Relations -- 6 The Death of History in British Sociology: Presentism, Intellectual Entrepreneurship, and the Conundra of Historical Consciousness -- 7 Sociology and Post-colonialism: Another 'Missing' Revolution? -- 8 Towards a Multiplication of Specialised Assemblages of Territory, Authority, and Rights -- 9 The Changing Life-Course in British Sociology -- 10 Aspirations and Opportunities: A Career in Sociology -- 11 Cracking the Ivory Tower: Proposing 'an Interpretive Public Sociology' -- 12 Sand in the Machine: Encouraging Academic Activism with Sociology HE Students Today -- 13 The Sociological Imagination As Popular Culture -- Index.

Note: Front Cover -- Atmospheric Transmission, Emission and Scattering -- Copyright Page -- Table of Contents -- Dedication -- Preface -- Acknowledgement -- Chapter 1. THE THERMODYNAMICS OF THE ATMOSPHERE -- Thermodynamics -- Chapter 2. MODELS OF THE ATMOSPHERE -- Atmospheric Structure and Processes -- Standard Models -- Chapter 3. AEROSOLS AND CLOUDS -- Aerosols -- Clouds -- Chapter 4. REFRACTION, POLARIZATION, AND AEROSOLS -- Polarization -- Refraction -- Dispersion -- Chapter 5. RAINBOWS, HALOS, AND SUCH -- Rainbows -- Halos and the Glory -- Chapter 6. SCATTERING -- Rayleigh Scattering -- Raman scattering -- The Mie Equations -- Computations and Measurements -- Chapter 7. TURBULENCE AND OPTICS -- The Inertial Subrange -- Optical Effects of Turbulence -- Structure Functions -- Refractivity Fluctuations -- Beam Effects -- Chapter 8. SPECTRA OF DIATOMIC MOLECULES -- Molecular Spectra -- Chapter 9. SYMMETRIC AND ASYMMETRIC TOP SPECTRA -- Linear Molecules -- Symmetric Top Molecules -- Asymmetric-top Molecules -- Electronic Transitions -- Chapter 10. LINE SHAPE FUNCTIONS -- Natural Line Width -- Doppler Broadening -- Pressure Broadening -- Collisions -- Other Line Shapes -- Combined Doppler and Lorentz Line Shape -- Atmospheric Line Shapes -- Curtis-Godsen Approximation -- Chapter 11. ABSORPTION AND EMISSION -- Absorption -- Band Models -- Random Line Models -- Emission -- Temperature Sounding by Satellite -- Chapter 12. MOLECULAR ABSORPTION BY SPECIES -- Nitrogen -- Oxygen -- Water Vapor -- Carbon Dioxide -- Ozone -- Other Molecules -- Chapter 13. COMPUTATIONAL TOOLS -- The AFGL Programs -- Fascode -- Other Software and Data -- References -- Subject Index -- About the Author.

Note: Front Cover -- Cell Separation: Methods and Selected Applications -- Copyright Page -- Table of Contents -- Preface -- Contents of Previous Volumes -- Chapter 1. Isolation and Characterization of Human Vascular Endothelial Cells with Application to Studies of the Subendothelial Matrix -- I. Introduction -- II. Isolation of Endothelial Cells -- III. Isolation and Characterization of the Subendothelial Matrix -- IV. Concluding Remarks -- REFERENCES -- Chapter 2. Separation and Characterization of Lymphoid Cells from Mouse Mammary Carcinomas by Isokinetic Sedimentation -- I. Introduction -- II. Separation of Lymphoid Cells from Mouse Mammary Tumors -- II. Separation of Lymphoid Cells from Mouse Mammary Tumors -- llΙ. Comparison of Results with Isokinetic Sedimentation and Centrifugal Elutriation -- IV. Conclusion -- REFERENCES -- Chapter 3. Separation of Malignant Cells from Host Cells Using Centrifugal Elutriation -- I. Introduction -- II. Theoretical and Practical Aspects -- III. Isolation of Host Cells from Tumor Cells -- IV. Isolation of Cell Subpopulations from Neoplastic Cells -- V. Conclusions -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 4. Selection of Tumor Cell Metastatic Variants by Differential Adhesion to Immobilized Lectins -- I. Introduction -- Il. Experimental Methods -- Ill. Fractionation of Murine Large-Cell Lymphoma Cells -- IV. Discussion of the Technique -- REFERENCES -- Chapter 5. Purification of Antigen-Specific Β Cells by Adherence to Whole-Cell Antigens -- I. Introduction -- II. Methods -- III. Assessment of the Technique -- IV. Comments on the Technique -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 6. Separation and Characterization of Subpopulations of Τ Cells -- I. Introduction -- II. Physiochemical Characteristics of Τ Lymphocytes -- IIΙ. Surface Markers of Τ Lymphocytes -- IV. Separation of Τ Lymphocyte Subpopulations. , V. Concluding Remarks -- REFERENCES -- Chapter 7. Separation of Pancreatic Islet Cells according to Functional Characteristics -- I. Introduction -- II. Isolation of Pancreatic Islets -- III. Preparation of Islet Cells -- IV. Autofluorescence-Activated Sorting of Islet Cells -- V. Applicability to Other Cells -- VI. Summary -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 8. Separation and Characterization of Leukocytes from the Intestine -- I. Introduction -- II. Distribution of Lymphoid Cells within the Intestine -- III. Isolation of Cells from Peyer's Patches -- IV. Isolation of Intraepithelial Leukocytes (IEL) -- V. Isolation of Cells from the Lamina Propria -- VI. Problems Associated with Isolation -- VII. Properties of Intestinal Leukocytes -- VIIl. Concluding Remarks -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 9. Separation of Epithelial Cells from Gastric and Intestinal Mucosa -- I. Introduction -- II. Techniques for Isolating Gastric and Intestinal Mucosal Cells -- III. Techniques for Cell Separation -- IV. Culture of Dispersed Gastrointestinal Epithelial Cells -- V. Morphology of Isolated Cells -- VI. Functional Characterization of Isolated Gastrointestinal Cells -- VII. Concluding Remarks -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 10. Separation of Cells from Normal and Abnormal Epidermis -- I. Introduction -- II. Methods of Epidermal Separation -- III. Methods of Epidermal Cell Separation -- IV. Biology and Pathobiology of Epidermal Cell Subpopulations -- V. Conclusion -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 11. Isokinetic Gradient Sedimentation of Hematopoietic Progenitor Cells -- I. Introduction -- II. Preparation of Bone Marrow Cells and Gradients -- III. Isokinetic Gradient Sedimentation of Mouse Bone Marrow Cells -- IV. Isokinetic Gradient Sedimentation of Human Bone Marrow Cells -- V. Concluding Remarks -- REFERENCES. , Chapter 12. Purification of Osteoblasts -- I. Introduction -- II. Separation of Osteoblasts -- III. Purification Methods -- IV. Problems of Separated Cells -- V. Identification of Osteoblast -- VI. Purification of Osteoblasts by FACS -- VII. Applications -- REFERENCES -- Chapter 13. Isolation and Separation of Human Colostral and Milk Cells -- I. Introduction -- II. Whole-Cell Populations -- III. Adherence -- IV. Isopycnic Centrifugation -- V. Velocity Sedimentation and Isokinetic Centrifugation -- VI. Evaluation of Various Techniques -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 14. Isokinetic Gradients for the Separation of Cells -- I. Introduction -- II. Background -- III. Isokinetic Sedimentation -- IV. Evaluation of Data -- V. Concluding Remarks -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 15. Analysis and Sorting by Flow Cytometry: Applications to the Study of Human Disease -- I. Introduction -- II. Technical Aspects of Flow Cytometry -- III. Sorting Applications in the Study of Human Disease -- IV. Appendix: Practical Aspects of Flow Sorting -- REFERENCES -- Chapter 16. Isolation of Granule-Rich and Granule-Deficient Cell Populations from Acinar Carcinoma of Rat Pancreas -- I. Transplantable Pancreatic Acinar Carcinoma -- II. Isolation of Granule-Rich and Granule-Deficient Carcinoma Cell Populations -- III. Perspectives -- ACKNOWLEDGMENTS -- REFERENCES -- INDEX.

Note: Cover -- Title -- Copyright -- Contents -- ACKNOWLEDGMENTS -- INTRODUCTION -- Chapter One: FEDERAL SCIENCE IN THE TRANS-MISSISSIPPI WEST AFTER 1865 -- Chapter Two: THE FOUNDING OF THE U. S. GEOLOGICAL SURVEY IN 1879 -- Chapter Three: A BUREAU OF MINES AND MINING -- Chapter Four: RESEARCH IN GEOLOGY AND PALEONTOLOGY -- Chapter Five: ADVANCEMENT IN TOPOGRAPHY: THE NATIONAL MAP -- Chapter Six: THE NEW POSITION OF PRACTICAL SCIENCE -- Chapter Seven: CONGRESS GRANTS PERMANENCE, 1886 -- Chapter Eight: YELLOWSTONE PARK: THE GEOLOGICAL SURVEY AND CONSERVATION -- Chapter Nine: THE IRRIGATION SURVEY: SCIENCE AND REFORM -- Chapter Ten: INTERVAL OF DECLINE, 1892-1894 -- Chapter Eleven: EPILOGUE: THE SURVEY IN THE TWENTIETH CENTURY -- NOTE ON SOURCES -- INDEX -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- Y -- MAPS -- Area of the Western Surveys after 1865 -- Area of Topographic Mapping in the South -- Yellowstone Park in the 1890's -- The Arid Region.

Note: Front Cover -- Cell Separation: Methods and Selected Applications -- Copyright Page -- Table of Contents -- Contributors -- Contents of Volume 1 -- Preface -- Chapter 1. Separation of Parafollicular Cells from Thyroid Follicular Cells by Affinity Chromatography Using Thyroglobulin-Coupled Sepharose -- I. Thyroid Structure and Function -- II. Parafollicular Cells and Serotonin -- III. Obtaining Parafollicular Cells for Study -- IV. Serotonin Binding Protein -- V. Distribution of Parafollicular Cells -- VI. Dissociation of the Cells of the Thyroid -- VII. Separation of Parafollicular Cells -- REFERENCES -- Chapter 2. Procurement and Purification of Eosinophils -- I. Introduction -- II. Procurement of Eosinophils -- III. Purification -- IV. Summary and Conclusions -- REFERENCES -- Chapter 3. Experimental Factors Involved in Separation by Centrifugal Elutriation -- I. Introduction -- II. Methodology of Elutriation -- III. Concluding Remarks -- REFERENCES -- Chapter 4. Analysis and Separation of Stromal Cells Infiltrating Tumors -- I. Introduction -- II. Previous Work -- III. Quantitation and Subcompartments -- IV. Identification of Stromal Cells and Morphometry -- V. Purification of Cells from Tumors -- VI. Concluding Comments -- REFERENCES -- Chapter 5. Selective Isolation of Epithelial Cells in Primary Expiant Cultures of Human and Animal Tissues -- I. Introduction -- II. Initiation of Primary Expiant Cultures -- III. Selective Isolation of Epithelial Cells -- IV. Identification of Human Bronchial and Bladder Epithelial Cells in Primary Expiant Cultures -- V. Discussion -- VI. Other Techniques -- REFERENCES -- Chapter 6. Isolation of Human Natural Killer Cells (Large Granular Lymphocytes) -- I. Introduction -- II. Isolation of Large Granular Lymphocytes (LGL) -- III. Comments -- REFERENCES -- Chapter 7. Purification of Islets and Cells from Islets. , I. Introduction -- II. Isolation of Pancreatic Islets -- III. Islet Purification -- IV. Preparation of Islet Cells -- V. Separation of Islet Cell Subpopulations -- VI. Summary -- REFERENCES -- Chapter 8. Magnetic Cell Sorting -- I. Introduction -- II. Magnetism and Magnetic Forces -- III. High Gradient Magnetic Separation -- IV. Magnetic Particles for Cell Labeling -- V. Conclusions -- REFERENCES -- Chapter 9. Separation of Cells by Preparative Density Gradient Electrophoresis -- I. Introduction -- II. Electrophoresis Theory -- III. Preparative Cell Electrophoresis in Density Gradients -- IV. Separation and Characterization of Cells by Density Gradient Electrophoresis -- V. Concluding Remarks -- REFERENCES -- Chapter 10. Isolation, Characterization, and Culture of Human Mammary Epithelial Cells -- I. Introduction -- II. Isolation of Mammary Epithelial Cells in Culture -- III. Method for the Culture of Human Mammary Epithelial Cells -- IV. Verification of the Mammary Epithelial Origin of the Cultured Cells -- V. Verification of the Malignant Origin of the Epithelial Cells Derived from Tumor Specimens -- VI. A Marker of Early Stages in Malignant Progression -- VII. Use of the Clonal Assay to Study Chemotherapeutic Drug Sensitivity -- VIII. Summary -- REFERENCES -- Chapter 11. Solid-Phase Fractionation of Lymphoid Cells on Ligand-Coated Plastic Plates -- I. Introduction -- II. Lymphocyte Fractionation -- III. Isolation of Surface Immunoglobulin-Positive Lymphocytes -- IV. Isolation of Human Fc Receptor-Bearing Lymphocytes on Antigen-Antibody Coated Dishes -- V. Isolation of Fc Receptor-Bearing Human T Lymphocytes -- VI. Isolation of Antigen-Specific Lymphocytes -- VII. Isolation of Lymphocyte Subsets with Monoclonal Antibodies -- VIII. Summary -- REFERENCES -- Chapter 12. Large Capacity Separation of Cells in a Reorienting Zonal Rotor -- I. Introduction. , II. General Characteristics of Reorienting Zonal Rotors -- III. Types of Separations Carried out in the TZ-28 Rotor -- IV. Choice of Reorienting Zonal Rotor versus Other Techniques -- V. Concluding Comments -- REFERENCES -- Chapter 13. Short-Term Culture of Cells from Human Solid Tumors in Semisolid Medium -- I. Introduction -- II. Methodology for Clonogenic Assays -- III. Conclusions -- REFERENCES -- Chapter 14. Preparation and Performance of the Erythrocyte-Unsaturated Fatty Acid (E-UFA) Test -- I. Historical Introduction -- II. Technique of Red Blood Cell (RBC) Preparation -- III. Measurement of Absolute Mobility -- IV. Precautions: Interfering Effects of Medication -- V. Some Unexpected Results -- VI. "Silent" and Subclinical Multiple Sclerosis -- VII. Etiology and Pathogenesis of Multiple Sclerosis -- VIII. Summary -- REFERENCES -- Chapter 15. Isolation of Erythroblastic Nests and Separation of Their Cellular Components -- I. Introduction -- II. Definitions -- IV. Method and Guidelines -- V. Generation of Erythropoietic Spleens Containing ENs -- VI. Preparation of Cell Suspensions from Erythropoietic Spleens -- VII. Preparation of Spleen Cell Suspensions Enriched in ENs -- VIII. EN Purification -- IX. EN Dissociation -- X. Separation of EN Central Cells from Erythroblasts -- XI. Summation of Methods and Selected Applications -- XII. Conclusions -- XIII. Perspectives for the Future -- REFERENCES -- Index.

Note: Front Cover -- Cell Separation: Methods and Selected Applications -- Copyright Page -- Table of Contents -- Preface -- Chapter 1. Separation and Characterization of Liver Cells -- I. Introduction -- II. Characterization of Liver Cells -- III. Preparation of Isolated Liver Cells -- IV. Conclusions -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 2. Purification and Characterization of Epidermal Langerhans Cells -- I. Introduction -- II. Cellular Constituents of the Epidermis -- III. Characterization of Epidermal Langerhans Cells -- IV. Enrichment of Epidermal Langerhans Cells -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 3. Purification and Culture of Oval Cells from Rat Liver -- I. Biological Importance of Oval Cells: Does Normal Adult Liver Contain Stem Cells? -- II. Isolation and Characterization of Oval Cells and Comparison with Other Liver Cell Types from Normal and Carcinogen-Treated Rats -- III. Growth of Liver Epithelial Cells in Vitro -- IV. Perspectives -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 4. Isolation and Characterization of Lymphocytes from Mature Mouse Liver -- I. Introduction -- II. Preparation of Suspensions of Cells from Liver -- III. Separation of Lymphocytes from Mouse Liver -- IV. Further Characterization of Lymphocytes from Liver -- V. Concluding Remarks -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 5. A Comparison of Cell Separations Obtained with Centrifugal Elutriation and Sedimentation at Unit Gravity -- I. Introduction -- II. A Theoretical Comparison and Factors Influencing Separation -- III. Applications -- IV. Summary and Conclusions -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 6. Limiting Dilution Assays for the Separation, Characterization, and Quantitation of Biologically Active Particles and Their Clonal Progeny -- I. Introduction -- II. Sample LDAs: SHPM Validity Tests -- III. Sample LDAs: Sample Estimators. , IV. Population LDAs -- V. Assay Design -- VI. Comparative Experiments -- VII. Clonal Analysis -- VIII. Partition Analysis -- IX. Conclusion -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 7. Purification and Functional Evaluation of Human Polymorphonuclear Leukocytes -- I. Introduction -- II. Background -- III. Techniques for the Isolation of Polymorphonuclear Neutrophils -- IV. Considerations in Choosing a Technique for the Purification of Polymorphonuclear Neutrophils -- V. Functional Analysis of Circulating Human Polymorphonuclear Neutrophils Isolated by Different Methods -- VI. Concluding Remarks -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 8. Fabrication of a Manually Operable Countercurrent Distribution Apparatus and Its Application for Separation of Cells in Aqueous Two-Phase Polymer System -- l. Introduction -- II. Countercurrent Distribution (CCD) -- III. Separation of Rat Testicular Cells by Manually Operable CCD Apparatus -- IV. Conclusion -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 9. Separation and Characterization of Phagocytes from Human Colon -- I. Introduction -- II. Dissociation of Mucosal Cells -- III. Enrichment of Mucosal Phagocytes -- IV. Analysis of Cell Suspensions by Flow Cytometry -- V. Functional Analysis -- VI. Concluding Remarks -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 10. Exploitation of Surface Molecules for Separation of Cells from Mosaic Livers -- I. Introduction -- II. Construction of Genotypic Mosaic Livers -- III. Immunological Approaches toward Purifying Donor- and Host-Origin Liver Cells from Genotypic Mosaic Livers -- IV. Summary -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 11. Isolation, Characterization, and Possible Functions of Follicular Dendritic Cells from Tonsils and Adenoids -- I. Introduction -- II. Characteristics of Follicular Dendritic Cells (FDC) in Situ -- III. Isolation of FDC. , IV. Characterization of Isolated FDC -- V. Functional Study of FDC in Vitro -- VI. Conclusions -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 12. Magnetite-Protein Conjugates for the Separation of Cells by High Gradient Magnetic Filtration -- I. Introduction -- II. Magnetic Filtration -- III. Particulate Magnetic Labels -- IV. Cell Labeling -- V. Cell Sorting -- VI. Summary -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 13. The Development of Techniques That Permit the Selection and Growth of Malignant Cells from Human Colonic Carcinomas -- I. Introduction -- II. Tissue Procurement and Initial Tissue Processing -- III. Human Colon Cancer Cell Lines -- IV. Characterization of Human Colon Cancer Cell Lines -- V. Utilization of a Human Cancer Cell Line Bank for Evaluation of New Anticancer Drugs -- VI. Concluding Remarks -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 14. Separation of Subpopulations from Heterogeneous Human Monocytes -- I. Introduction -- II. Separation Procedures Currently Available to Isolate Monocytes -- III. Isolation of Monocyte Subsets by Means of a Blood Component Separator (BCS) and Centrifugal Elutriation (CE) -- IV. Phenotypic Analysis of the Monocyte Subsets -- V. Functional Analysis of the Monocyte Subsets -- VI. Conclusions -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 15. Identification and Isolation of Human Splenic Macrophages, Lymphocytes, and Related Cells with in Situ Immunohistochemical Techniques and Countercurrent Centrifugal Elutriation -- I. Introduction -- II. Identification of Various Types of Spleen Cells in Situ -- III. Preparation of Spleen Cell Suspensions -- IV. Separation of Spleen Cells by Countercurrent Centrifugal Elutriation -- V. Critical Assessment of the Methods Used to Identify Spleen Cell Types and to Separate Them from Splenic Tissue -- ACKNOWLEDGMENTS -- REFERENCES. , Chapter 16. Methods for the Purification of Malignant Cells from Blood -- I. Introduction -- II. General Considerations -- III. Separation Procedures -- IV. Nonseparative Procedures -- V. Conclusions -- REFERENCES -- Chapter 17. Heterogeneity of Proteolytic Enzyme Preparations Commonly Employed for Dispersal of Solid Tissues -- I. Introduction -- II. Enzymatic Dispersal of Solid Tissues -- III. Trypsin -- IV. Heterogeneity of Enzyme Preparations Used for Tissue Dispersal -- V. Recommendations for Increased Standardization of Tissue Dispersal -- REFERENCES -- Index.